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Rabbit Recombinant Monoclonal ABCB6/PRP antibody. Carrier free. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - Anti-ABCB6/PRP antibody [EPR21891] - BSA and Azide free (AB236457), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ABCB6/PRP antibody [EPR21891] - BSA and Azide free (AB236457), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ABCB6/PRP antibody [EPR21891] - BSA and Azide free (AB236457), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow Cyt (Intra)
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

ATP-dependent transporter that catalyzes the transport of a broad-spectrum of porphyrins from the cytoplasm to the extracellular space through the plasma membrane or into the vesicle lumen (PubMed:17661442, PubMed:23792964, PubMed:27507172, PubMed:33007128). May also function as an ATP-dependent importer of porphyrins from the cytoplasm into the mitochondria, in turn may participate in the de novo heme biosynthesis regulation and in the coordination of heme and iron homeostasis during phenylhydrazine stress (PubMed:10837493, PubMed:17006453, PubMed:23792964, PubMed:33007128). May also play a key role in the early steps of melanogenesis producing PMEL amyloid fibrils (PubMed:29940187). In vitro, it confers to cells a resistance to toxic metal such as arsenic and cadmium and against chemotherapeutics agent such as 5-fluorouracil, SN-38 and vincristin (PubMed:21266531, PubMed:25202056, PubMed:31053883). In addition may play a role in the transition metal homeostasis (By similarity).

Alternative names

MTABC3, PRP, UMAT, ABCB6, ATP-binding cassette sub-family B member 6, ABC-type heme transporter ABCB6, Mitochondrial ABC transporter 3, P-glycoprotein-related protein, Ubiquitously-expressed mammalian ABC half transporter, Mt-ABC transporter 3

Recommended products

Rabbit Recombinant Monoclonal ABCB6/PRP antibody. Carrier free. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR21891
Purification technique
Affinity purification Protein A
Concentration
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Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab236457 is the carrier-free version of Anti-ABCB6/PRP antibody [EPR21891] ab221159.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ABCB6 also known as PRP is a transporter protein that plays important roles in cellular processes. This protein is part of the ATP-binding cassette (ABC) transporters specifically the B subfamily which are known for moving various molecules across membranes. ABCB6 has a molecular mass of approximately 92 kDa. It is expressed in various tissues including the liver and erythroid cells indicating its widespread importance in the body.

Biological function summary

The ABCB6 protein is involved in the transport of porphyrins and other tetrapyrroles across membranes. These molecules are important for heme biosynthesis impacting red blood cell production and function. ABCB6 functions as a homodimer and is located on mitochondrial and lysosomal membranes. The transporter's ability to influence heme production links it to essential cellular metabolic processes.

Pathways

The ABCB6 protein is engaged in the heme biosynthesis and mitochondrial function pathways. Its activity supports the synthesis of heme a fundamental component of hemoglobin and other heme-containing proteins. ABCB6 interacts with other proteins such as ALAS2 the erythroid-specific isoform of delta-aminolevulinic acid synthase regulating heme synthesis in erythroid cells. This connection highlights its role in maintaining adequate heme levels within cells.

Associated diseases and disorders

ABCB6 has been implicated in conditions such as porphyria and anemia. Porphyria is a group of disorders stemming from defects in heme biosynthesis where mutations in ABCB6 may disrupt porphyrin transport aggravating symptoms. Anemia can also arise from impaired ABCB6 function as inadequate heme production affects red blood cell development. In these contexts ABCB6 can connect with proteins like ferrochelatase which plays a role in the final step of heme production illustrating the impact of ABCB6 on disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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