AB151992

Anti-ABCB7 antibody

Name: Anti-ABCB7 antibody (ab151992) | Abcam
Brand: Abcam Limited
SKU: AB151992
Price: 535 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal ABCB7 antibody. Suitable for WB and reacts with Human, Drosophila melanogaster samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ABCB7 aa 400-750.

View Alternative Names

ABC7, ABCB7, ATP-binding cassette transporter 7, ABC transporter 7 protein

2 Images

Supplier Data

Western blot - Anti-ABCB7 antibody (AB151992)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-ABCB7 antibody (ab151992) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 83 kDa

false

Supplier Data

Western blot - Anti-ABCB7 antibody (AB151992)

Samples were separated by 7.5% SDS PAGE. The signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-ABCB7 antibody (ab151992) at 1/1000 dilution

All lanes:

S2 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 83 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Drosophila melanogaster

Applications

applications

Immunogen

Recombinant Fragment Protein within Human ABCB7 aa 400-750. The exact immunogen used to generate this antibody is proprietary information.

O75027

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCB7 also known as ATP-binding cassette sub-family B member 7 plays an essential role as a transporter protein involved in the transfer of iron-sulfur clusters from mitochondria to the cytosol. This protein has a mass of approximately 82 kDa and is mainly expressed in human tissues including the bone marrow and liver. The ABCB7 protein contains two nucleotide-binding domains and two transmembrane domains which facilitate its function as a processive pump across the membrane.

Biological function summary

ABCB7 is significant in maintaining cellular iron homeostasis. It integrates into a functional complex responsible for maturing iron-sulfur clusters that are critical for various cellular activities. These iron-sulfur clusters participate in numerous biochemical functions including electron transport and enzyme catalysis. Without their proper supply several cellular processes are compromised leading to metabolic dysfunctions.

Pathways

ABCB7 is integral to the biosynthesis of iron-sulfur clusters and their subsequent incorporation into proteins. This pathway involves essential collaborations with proteins such as frataxin and NFS1. The protein's role in this process affects multiple cellular processes including the mitochondrial electron transport chain and the regulation of cellular oxidative stress which are key components in the maintenance of cellular energy and redox states.

Mutations or malfunctions in ABCB7 can lead to conditions such as X-linked sideroblastic anemia and ataxia (XLSA/A). XLSA/A is marked by defective heme synthesis and increased risk of anemia due to disrupted iron-sulfur cluster biogenesis. In these diseases ABCB7 maintains direct associations with both ABCB6 and mitoferrin proteins that also play roles in iron metabolism. The genetic defects leading to dysfunctions in the ABCB7 protein result in severe metabolic consequences due to the impaired iron-sulfur cluster assembly.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Exports glutathione-coordinated iron-sulfur clusters such as [2Fe-2S]-(GS)4 cluster from the mitochondria to the cytosol in an ATP-dependent manner allowing the assembly of the cytosolic iron-sulfur (Fe/S) cluster-containing proteins and participates in iron homeostasis (PubMed : 10196363, PubMed : 17192393, PubMed : 33157103). Moreover, through a functional complex formed of ABCB7, FECH and ABCB10, also plays a role in the cellular iron homeostasis, mitochondrial function and heme biosynthesis (PubMed : 30765471). In cardiomyocytes, regulates cellular iron homeostasis and cellular reactive oxygen species (ROS) levels through its interaction with COX4I1 (By similarity). May also play a role in hematopoiesis (By similarity).

See full target information ABCB7

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 4:138 PubMed33514783

2021

Cardiac-specific loss of mitoNEET expression is linked with age-related heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Takaaki Furihata,Shingo Takada,Naoya Kakutani,Satoshi Maekawa,Masaya Tsuda,Junichi Matsumoto,Wataru Mizushima,Arata Fukushima,Takashi Yokota,Nobuyuki Enzan,Shouji Matsushima,Haruka Handa,Yoshizuki Fumoto,Junko Nio-Kobayashi,Toshihiko Iwanaga,Shinya Tanaka,Hiroyuki Tsutsui,Hisataka Sabe,Shintaro Kinugawa

Scientific reports 10:13988 PubMed32814794

2020

Hedgehog-GLI signalling promotes chemoresistance through the regulation of ABC transporters in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Agnese Po,Anna Citarella,Giuseppina Catanzaro,Zein Mersini Besharat,Sofia Trocchianesi,Francesca Gianno,Claudia Sabato,Marta Moretti,Enrico De Smaele,Alessandra Vacca,Micol Eleonora Fiori,Elisabetta Ferretti

3 Biotech 10:173 PubMed32206507

2020

Molecular evidence of impaired iron metabolism and its association with Parkinson's disease progression.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Chi,Wei Tang,Ying Bai

Haematologica 104:1756-1767 PubMed30765471

2019

Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Nunziata Maio,Ki Soon Kim,Gregory Holmes-Hampton,Anamika Singh,Tracey A Rouault

Cell metabolism 25:945-953.e6 PubMed28380382

2017

A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III.

Applications

Unspecified application

Species

Unspecified reactive species

Nunziata Maio,Ki Soon Kim,Anamika Singh,Tracey A Rouault

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ABCB7 antibody

Western blot - Anti-ABCB7 antibody (AB151992)

Western blot - Anti-ABCB7 antibody (AB151992)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise