Rabbit Polyclonal ABCB7 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ABCB7 aa 400-750.
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Publications
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- Communications biology 4:1382021Cardiac-specific loss of mitoNEET expression is linked with age-related heart failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakaaki Furihata,Shingo Takada,Naoya Kakutani,Satoshi Maekawa,Masaya Tsuda,Junichi Matsumoto,Wataru Mizushima,Arata Fukushima,Takashi Yokota,Nobuyuki Enzan,Shouji Matsushima,Haruka Handa,Yoshizuki Fumoto,Junko Nio-Kobayashi,Toshihiko Iwanaga,Shinya Tanaka,Hiroyuki Tsutsui,Hisataka Sabe,Shintaro KinugawaPubMed 33514783
- Scientific reports 10:139882020Hedgehog-GLI signalling promotes chemoresistance through the regulation of ABC transporters in colorectal cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgnese Po et. al.PubMed 32814794
- 3 Biotech 10:1732020Molecular evidence of impaired iron metabolism and its association with Parkinson's disease progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaitao Chi et. al.PubMed 32206507
- Haematologica 104:1756-17672019Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNunziata Maio et. al.PubMed 30765471
- Cell metabolism 25:945-953.e62017A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNunziata Maio,Ki Soon Kim,Anamika Singh,Tracey A RouaultPubMed 28380382
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ABCB7 aa 400-750. The exact immunogen used to generate this antibody is proprietary information. Database link O75027
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Cow | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Target data
Function
Exports glutathione-coordinated iron-sulfur clusters such as [2Fe-2S]-(GS)4 cluster from the mitochondria to the cytosol in an ATP-dependent manner allowing the assembly of the cytosolic iron-sulfur (Fe/S) cluster-containing proteins and participates in iron homeostasis (PubMed:10196363, PubMed:17192393, PubMed:33157103). Moreover, through a functional complex formed of ABCB7, FECH and ABCB10, also plays a role in the cellular iron homeostasis, mitochondrial function and heme biosynthesis (PubMed:30765471). In cardiomyocytes, regulates cellular iron homeostasis and cellular reactive oxygen species (ROS) levels through its interaction with COX4I1 (By similarity). May also play a role in hematopoiesis (By similarity).
Alternative names
ABC7, ABCB7, ATP-binding cassette transporter 7, ABC transporter 7 protein
Recommended products
Rabbit Polyclonal ABCB7 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ABCB7 aa 400-750.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ABCB7 aa 400-750. The exact immunogen used to generate this antibody is proprietary information. Database link O75027
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ABCB7 also known as ATP-binding cassette sub-family B member 7 plays an essential role as a transporter protein involved in the transfer of iron-sulfur clusters from mitochondria to the cytosol. This protein has a mass of approximately 82 kDa and is mainly expressed in human tissues including the bone marrow and liver. The ABCB7 protein contains two nucleotide-binding domains and two transmembrane domains which facilitate its function as a processive pump across the membrane.
- Biological function summary
ABCB7 is significant in maintaining cellular iron homeostasis. It integrates into a functional complex responsible for maturing iron-sulfur clusters that are critical for various cellular activities. These iron-sulfur clusters participate in numerous biochemical functions including electron transport and enzyme catalysis. Without their proper supply several cellular processes are compromised leading to metabolic dysfunctions.
- Pathways
ABCB7 is integral to the biosynthesis of iron-sulfur clusters and their subsequent incorporation into proteins. This pathway involves essential collaborations with proteins such as frataxin and NFS1. The protein's role in this process affects multiple cellular processes including the mitochondrial electron transport chain and the regulation of cellular oxidative stress which are key components in the maintenance of cellular energy and redox states.
- Associated diseases and disorders
Mutations or malfunctions in ABCB7 can lead to conditions such as X-linked sideroblastic anemia and ataxia (XLSA/A). XLSA/A is marked by defective heme synthesis and increased risk of anemia due to disrupted iron-sulfur cluster biogenesis. In these diseases ABCB7 maintains direct associations with both ABCB6 and mitoferrin proteins that also play roles in iron metabolism. The genetic defects leading to dysfunctions in the ABCB7 protein result in severe metabolic consequences due to the impaired iron-sulfur cluster assembly.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-ABCB7 antibody (ab151992)
7.5% SDS PAGE
All lanes: Western blot - Anti-ABCB7 antibody (ab151992) at 1/1000 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 83 kDa
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