AB174629

Anti-ABCC9 antibody [N319A/14] - C-terminal

Name: Anti-ABCC9 antibody [N319A/14] - C-terminal (ab174629) | Abcam
Brand: Abcam Limited
SKU: AB174629
Price: 505 USD
Availability: InStock

Be the first to review this product! Submit a review

(6 Publications)

Mouse Monoclonal ABCC9 antibody. C-terminal. Suitable for WB, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Abcc9 aa 1500 to C-terminus.

View Alternative Names

SUR2, ABCC9, ATP-binding cassette sub-family C member 9, Sulfonylurea receptor 2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCC9 antibody [N319A/14] - C-terminal (AB174629)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCC9 antibody [N319A/14] - C-terminal (AB174629)

4% formaldehyde-fixed SK-N-BE cells labeling ABCC9 using ab174629 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/100 dilution incubated for 1 hour at room temperature. Counter stained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).

Supplier Data

Western blot - Anti-ABCC9 antibody [N319A/14] - C-terminal (AB174629)

All lanes:

Western blot - Anti-ABCC9 antibody [N319A/14] - C-terminal (ab174629) at 1/1000 dilution

All lanes:

Rat brain membrane lysate at 10 µg

Secondary

All lanes:

Goat anti-mouse IgG at 1/200 dilution

Predicted band size: 174 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N319A/14

Isotype

IgG2a

Carrier free

Reacts with

Rat, Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Abcc9 aa 1500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P70170

Specificity

ab174629 does not crossreact with SUR2B.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

The clone number has been updated from S319A-14 to N319A/14, both clone numbers name the same antibody clone.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCC9 also known as SUR2 (sulfonylurea receptor 2) is a protein part of the ATP-binding cassette transporter superfamily. It weighs approximately 174 kDa. The protein functions as a regulatory subunit of the KATP potassium channel controlling ion flow through cell membranes. ABCC9 is present in various tissues with significant expression in cardiac and skeletal muscles and in pancreatic cells.

Biological function summary

ABCC9 plays an important role in the response to metabolic changes by linking cellular excitability to metabolism. As a component of the KATP channel complex it regulates the channel's open and closing states in response to intracellular ATP levels. This regulation impacts muscle contraction and insulin release significant processes in energy maintenance and homeostasis.

Pathways

ABCC9 is integrated into significant signaling networks such as the insulin secretion pathway and the cardiac muscle contraction pathway. It interacts with proteins like Kir6.2 a potassium channel protein forming the pore of KATP channels. These pathways illustrate ABCC9's involvement in glucose homeostasis and cardiac rhythm regulation.

ABCC9 is linked to heart disorders such as dilated cardiomyopathy and metabolic conditions like hyperinsulinism. Mutations or dysregulation of ABCC9 affect its interaction with proteins like Kir6.2 influencing cardiac excitability and insulin secretion. The dysfunction of this protein could result in abnormal heart rhythms and impaired insulin regulation contributing to the mentioned diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Subunit of ATP-sensitive potassium channels (KATP). Can form cardiac and smooth muscle-type KATP channels with KCNJ11. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation (PubMed : 9831708). Can form a sulfonylurea-sensitive but ATP-insensitive potassium channel with KCNJ8 (By similarity).

See full target information ABCC9

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 12:1656718 PubMed41020224

2025

Gain-of-function mutations in KATP channel subunits compromise colonic tight junction integrity and epithelial homeostasis in murine models of Cantú syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Fatima Maqoud,Domenica Mallardi,Antonella Orlando,Domenico Tricarico,Colin G Nichols,Marina Antonacci,Giusy Bianco,Raffaele Armentano,Ilaria Grassi,Anna Maria Valentini,Francesco Russo

International journal of molecular sciences 25: PubMed38791311

2024

miRNA Expression Profiles in Isolated Ventricular Cardiomyocytes: Insights into Doxorubicin-Induced Cardiotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Yohana Domínguez Romero,Gladis Montoya Ortiz,Susana Novoa Herrán,Jhon Osorio Mendez,Luis A Gomez Grosso

Frontiers in pharmacology 14:1115543 PubMed37180726

2023

Immunohistochemical, pharmacovigilance, and omics analyses reveal the involvement of ATP-sensitive K channel subunits in cancers: role in drug-disease interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Fatima Maqoud,Nicola Zizzo,Marcella Attimonelli,Antonella Tinelli,Giuseppe Passantino,Marina Antonacci,Girolamo Ranieri,Domenico Tricarico

American journal of physiology. Renal physiology 324:F168-F178 PubMed36454699

2022

Nicorandil protects podocytes via modulation of antioxidative capacity in acute puromycin aminonucleoside-induced nephrosis in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Masaki Yamanaka,Yoshifuru Tamura,Emiko Kuribayashi-Okuma,Shunya Uchida,Shigeru Shibata

Acta pharmacologica Sinica 43:1349-1359 PubMed34697419

2021

Iptakalim improves cerebral microcirculation in mice after ischemic stroke by inhibiting pericyte contraction.

Applications

Unspecified application

Species

Unspecified reactive species

Ruo-Bing Guo,Yin-Feng Dong,Zhi Yin,Zhen-Yu Cai,Jin Yang,Juan Ji,Yu-Qin Sun,Xin-Xin Huang,Teng-Fei Xue,Hong Cheng,Xi-Qiao Zhou,Xiu-Lan Sun

Frontiers in pharmacology 7:315 PubMed27721792

2016

An NBD Derivative of the Selective Rat Toxicant Norbormide as a New Probe for Living Cell Imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Claudio D'Amore,Genny Orso,Fabio Fusi,Mario A Pagano,Giovanni Miotto,Alessia Forgiarini,Sara De Martin,Giulia Castellani,Giovanni Ribaudo,David Rennison,Margaret A Brimble,Brian Hopkins,Alessandro Ferrarese,Sergio Bova

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ABCC9 antibody [N319A/14] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCC9 antibody [N319A/14] - C-terminal (AB174629)

Western blot - Anti-ABCC9 antibody [N319A/14] - C-terminal (AB174629)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise