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AB197013

Anti-ABCD1/ALD antibody [EPR15929]

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(1 Review)

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(10 Publications)

Anti-ABCD1/ALD antibody [EPR15929] (ab197013) is a rabbit monoclonal antibody detecting ABCD1/ALD in Western Blot, Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

ALD, ABCD1, ATP-binding cassette sub-family D member 1, Adrenoleukodystrophy protein, ALDP

5 Images
Flow Cytometry (Intracellular) - Anti-ABCD1/ALD antibody [EPR15929] (AB197013)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-ABCD1/ALD antibody [EPR15929] (AB197013)

ab197013 staining ABCD1/ ALD in the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. The sample was incubated with the primary antibody at a dilution of 1/70. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control : Rabbit monoclonal IgG (Black)

Unlabelled control : Cell without incubation with primary antibody and secondary antibody (Blue)

Western blot - Anti-ABCD1/ALD antibody [EPR15929] (AB197013)
  • WB

Supplier Data

Western blot - Anti-ABCD1/ALD antibody [EPR15929] (AB197013)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ABCD1/ALD antibody [EPR15929] (ab197013) at 1/10000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

Human fetal liver lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 82 kDa

Observed band size: 83 kDa

false

Western blot - Anti-ABCD1/ALD antibody [EPR15929] (AB197013)
  • WB

Supplier Data

Western blot - Anti-ABCD1/ALD antibody [EPR15929] (AB197013)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ABCD1/ALD antibody [EPR15929] (ab197013) at 1/10000 dilution

All lanes:

JAR (Human placenta choriocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 82 kDa

Observed band size: 83 kDa

false

Western blot - Anti-ABCD1/ALD antibody [EPR15929] (AB197013)
  • WB

Supplier Data

Western blot - Anti-ABCD1/ALD antibody [EPR15929] (AB197013)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ABCD1/ALD antibody [EPR15929] (ab197013) at 1/10000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 82 kDa

Observed band size: 83 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-ABCD1/ALD antibody [EPR15929] (AB197013)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ABCD1/ALD antibody [EPR15929] (AB197013)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling ABCD1 / ALD with ab197013 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF7 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows :
-ve control 1 : ab197013 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15929

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-ABCD1/ALD antibody [EPR15929] (ab197013) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of ABCD1/ALD?
Anti-ABCD1/ALD [EPR15929] (ab197013) specifically detects a band for ABCD1/ALD (UniProt: P33897) at a molecular weight of 83kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR15929] also available for your convenience: ab197013, Alexa Fluor® 647 - ab225409, Alexa Fluor® 488 - ab225410, Carrier free - ab238989

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCD1 also known as the ABCD1 protein or ALD protein is encoded by the ABCD1 gene and belongs to the ATP-binding cassette (ABC) transporter family. It functions mechanically by transporting very long-chain fatty acids (VLCFAs) into peroxisomes where they can be metabolized. ABCD1 has a mass of approximately 80 kDa. Expression of ABCD1 is significant in tissues such as the liver adrenal glands and brain where it plays a role in lipid metabolism and cellular detoxification processes.
Biological function summary

ABCD1 contributes to the maintenance of normal fatty acid levels within cells. It is not known as part of a larger protein complex but it cooperates closely with other peroxisomal enzymes to facilitate the beta-oxidation of VLCFAs. This action ensures the breakdown of toxic compounds and has a significant role in maintaining cellular homeostasis. The proper function of ABCD1 is essential for preventing the accumulation of VLCFAs which can affect cellular function and viability.

Pathways

ABCD1 is integral to the metabolic pathway of peroxisomal beta-oxidation. This pathway is important for the degradation of excess VLCFAs which are otherwise difficult to break down in mitochondria. ABCD1 works in concert with related proteins such as ABCD2 and ABCD3 playing differentiated yet sometimes overlapping roles in lipid transport and metabolism. This cooperation is important for maintaining normal cellular metabolism and preventing lipid accumulation.

Defects in ABCD1 are directly associated with X-linked adrenoleukodystrophy (X-ALD) a neurodegenerative disorder characterized by the accumulation of VLCFAs in tissues. This condition leads to severe neurological impairment due to the toxic buildup of these lipids which disrupts normal brain function. Additionally the ABCD1 protein's dysfunction can indirectly affect the function of other proteins involved in lipid metabolism potentially exacerbating conditions related to peroxisomal disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-dependent transporter of the ATP-binding cassette (ABC) family involved in the transport of very long chain fatty acid (VLCFA)-CoA from the cytosol to the peroxisome lumen (PubMed : 11248239, PubMed : 15682271, PubMed : 16946495, PubMed : 18757502, PubMed : 21145416, PubMed : 23671276, PubMed : 29397936, PubMed : 33500543). Coupled to the ATP-dependent transporter activity has also a fatty acyl-CoA thioesterase activity (ACOT) and hydrolyzes VLCFA-CoA into VLCFA prior their ATP-dependent transport into peroxisomes, the ACOT activity is essential during this transport process (PubMed : 29397936, PubMed : 33500543). Thus, plays a role in regulation of VLCFAs and energy metabolism namely, in the degradation and biosynthesis of fatty acids by beta-oxidation, mitochondrial function and microsomal fatty acid elongation (PubMed : 21145416, PubMed : 23671276). Involved in several processes; namely, controls the active myelination phase by negatively regulating the microsomal fatty acid elongation activity and may also play a role in axon and myelin maintenance. Controls also the cellular response to oxidative stress by regulating mitochondrial functions such as mitochondrial oxidative phosphorylation and depolarization. And finally controls the inflammatory response by positively regulating peroxisomal beta-oxidation of VLCFAs (By similarity).
See full target information ABCD1
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Journal of inherited metabolic disease 48:e12832 PubMed39704488

2024

Altered lipid profile and reduced neuronal support in human induced pluripotent stem cell-derived astrocytes from adrenoleukodystrophy patients.

Applications

Unspecified application

Species

Unspecified reactive species

Roberto Montoro Ferrer,Yorrick R J Jaspers,Inge M E Dijkstra,Nicole Breeuwsma,Jan-Bert van Klinken,Cato Romero,Marc Engelen,Stephan Kemp,Vivi M Heine

Nature cell biology 26:1261-1273 PubMed38969763

2024

Functional multi-organelle units control inflammatory lipid metabolism of macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Julia A Zimmermann,Kerstin Lucht,Manuel Stecher,Chahat Badhan,Katharina M Glaser,Maximilian W Epple,Lena R Koch,Ward Deboutte,Thomas Manke,Klaus Ebnet,Frauke Brinkmann,Olesja Fehler,Thomas Vogl,Ev-Marie Schuster,Anna Bremser,Joerg M Buescher,Angelika S Rambold

The American journal of surgical pathology 48:511-520 PubMed38567813

2024

ABCD1 as a Novel Diagnostic Marker for Solid Pseudopapillary Neoplasm of the Pancreas.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Ao Liu,Yuanhao Liu,Jiajuan Tu,Yihong Shi,Junyi Pang,Qi Huang,Xun Wang,Zhixiang Lin,Yupei Zhao,Wenze Wang,Junya Peng,Wenming Wu

The Journal of cell biology 222: PubMed37737955

2023

Peroxisomal ROS control cytosolic Mycobacterium tuberculosis replication in human macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Enrica Pellegrino,Beren Aylan,Claudio Bussi,Antony Fearns,Elliott M Bernard,Natalia Athanasiadi,Pierre Santucci,Laure Botella,Maximiliano G Gutierrez

Journal of lipid research 64:100364 PubMed36990386

2023

The origin of long-chain fatty acids required for de novo ether lipid/plasmalogen synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Serhii Chornyi,Rob Ofman,Janet Koster,Hans R Waterham

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 43:370-378 PubMed36880961

2023

A Novel Inactive Isoform with a Restored Reading Frame Is Expressed from the Human Interferon Lambda 4 TT Allele at rs368234815.

Applications

Unspecified application

Species

Unspecified reactive species

Seema Bharatiya,Aditya Agarwal,Sreedhar Chinnaswamy

Cells 11: PubMed35681537

2022

Peroxisome Metabolism Contributes to PIEZO2-Mediated Mechanical Allodynia.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Gong,Fiza Laheji,Anna Berenson,April Qian,Sang-O Park,Rene Kok,Martin Selig,Ryan Hahn,Reza Sadjadi,Stephan Kemp,Florian Eichler

Stem cell research 53:102337 PubMed33901816

2021

Generation of two human induced pluripotent stem cell lines derived from two X-linked adrenoleukodystrophy patients with ABCD1 mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Yuji Kuramochi,Tomonari Awaya,Mami Matsuo-Takasaki,Miho Takami,Yuri An,Jingyue Li,Yasuko Hemmi,Tamami Wakabayashi,Yutaka Arai,Jun Inoue,Michiya Noguchi,Yukio Nakamura,Isao Asaka,Kazunori Akimoto,Megumu K Saito,Yohei Hayashi

The Journal of cell biology 218:2583-2599 PubMed31227594

2019

Spastin tethers lipid droplets to peroxisomes and directs fatty acid trafficking through ESCRT-III.

Applications

Unspecified application

Species

Unspecified reactive species

Chi-Lun Chang,Aubrey V Weigel,Maria S Ioannou,H Amalia Pasolli,C Shan Xu,David R Peale,Gleb Shtengel,Melanie Freeman,Harald F Hess,Craig Blackstone,Jennifer Lippincott-Schwartz

Human gene therapy 30:544-555 PubMed30358470

2018

Intrathecal Adeno-Associated Viral Vector-Mediated Gene Delivery for Adrenomyeloneuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Gong,Anna Berenson,Fiza Laheji,Guangping Gao,Dan Wang,Carrie Ng,Adrienn Volak,Rene Kok,Vasileios Kreouzis,Inge M Dijkstra,Stephan Kemp,Casey A Maguire,Florian Eichler
View all publications
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