Rabbit Polyclonal ABCD4 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ABCD4.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ABCD4. The exact immunogen used to generate this antibody is proprietary information. Database link O14678
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Lysosomal membrane protein that transports cobalamin (Vitamin B12) from the lysosomal lumen to the cytosol in an ATP-dependent manner (PubMed:22922874, PubMed:28572511, PubMed:31467407, PubMed:33845046). Targeted by LMBRD1 lysosomal chaperone from the endoplasmic reticulum to the lysosomal membrane (PubMed:27456980). Then forms a complex with lysosomal chaperone LMBRD1 and cytosolic MMACHC to transport cobalamin across the lysosomal membrane (PubMed:25535791).
Alternative names
PXMP1L, ABCD4, Lysosomal cobalamin transporter ABCD4, ATP-binding cassette sub-family D member 4, PMP70-related protein, Peroxisomal membrane protein 1-like, Peroxisomal membrane protein 69, P70R, PXMP1-L, PMP69
Recommended products
Rabbit Polyclonal ABCD4 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ABCD4.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ABCD4. The exact immunogen used to generate this antibody is proprietary information. Database link O14678
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ABCD4 or ATP-binding cassette sub-family D member 4 functions as a transport protein involved in intracellular lipid trafficking. It belongs to the ATP-binding cassette (ABC) family which utilizes ATP hydrolysis to transport various substrates across cellular membranes. The molecular weight of ABCD4 is approximately 75 kilodaltons. It most commonly expresses itself in human tissues such as the brain liver kidney and heart. These areas suggest that ABCD4 plays essential roles in these organs' lipid-related processes.
- Biological function summary
ABCD4 facilitates the transport of cobalamin (vitamin B12) from lysosomes to the cytoplasm. It operates as part of a multi-protein complex that includes LMBD1 and CBLIF. The complex collectively ensures cobalamin accessibility for cellular metabolic reactions. A proper transport function is critical for supporting the synthesis of DNA and maintaining normal hematopoiesis through vitamin B12 processing.
- Pathways
The protein facilitates integration into the cobalamin metabolism pathway and the peroxisomal biogenesis pathway. ABCD4 closely interacts with proteins such as LMBD1 in the former pathway. It helps import mediated transport which is integral for B12 availability affecting subsequent protein activity like methionine synthase and methylmalonyl-CoA mutase. These activities ensure vital cellular processes such as nucleotide biosynthesis and lipid metabolism proceed smoothly.
- Associated diseases and disorders
ABCD4 mutations link to inborn errors like methylmalonic aciduria and vitamin B12 deficiency anemia. These conditions arise from impaired cobalamin processing due to dysfunctional transport activity. In particular during B12 deficiency anemia ABCD4’s interaction with vitamin B12 transport proteins such as LMBD1 results in inadequate vitamin transport leading to disrupted hematopoietic function and metabolic imbalances.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-ABCD4 antibody (ab135480)
All lanes: Western blot - Anti-ABCD4 antibody (ab135480) at 1/500 dilution
All lanes: Lysates from HeLa cells at 30 µg
Predicted band size: 69 kDa
Downloads
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