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AB135480

Anti-ABCD4 antibody

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(1 Publication)

Rabbit Polyclonal ABCD4 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ABCD4.

View Alternative Names

PXMP1L, ABCD4, Lysosomal cobalamin transporter ABCD4, ATP-binding cassette sub-family D member 4, PMP70-related protein, Peroxisomal membrane protein 1-like, Peroxisomal membrane protein 69, P70R, PXMP1-L, PMP69

1 Images
Western blot - Anti-ABCD4 antibody (AB135480)
  • WB

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Western blot - Anti-ABCD4 antibody (AB135480)

All lanes:

Western blot - Anti-ABCD4 antibody (ab135480) at 1/500 dilution

All lanes:

Lysates from HeLa cells at 30 µg

Predicted band size: 69 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ABCD4. The exact immunogen used to generate this antibody is proprietary information.

O14678

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCD4 or ATP-binding cassette sub-family D member 4 functions as a transport protein involved in intracellular lipid trafficking. It belongs to the ATP-binding cassette (ABC) family which utilizes ATP hydrolysis to transport various substrates across cellular membranes. The molecular weight of ABCD4 is approximately 75 kilodaltons. It most commonly expresses itself in human tissues such as the brain liver kidney and heart. These areas suggest that ABCD4 plays essential roles in these organs' lipid-related processes.
Biological function summary

ABCD4 facilitates the transport of cobalamin (vitamin B12) from lysosomes to the cytoplasm. It operates as part of a multi-protein complex that includes LMBD1 and CBLIF. The complex collectively ensures cobalamin accessibility for cellular metabolic reactions. A proper transport function is critical for supporting the synthesis of DNA and maintaining normal hematopoiesis through vitamin B12 processing.

Pathways

The protein facilitates integration into the cobalamin metabolism pathway and the peroxisomal biogenesis pathway. ABCD4 closely interacts with proteins such as LMBD1 in the former pathway. It helps import mediated transport which is integral for B12 availability affecting subsequent protein activity like methionine synthase and methylmalonyl-CoA mutase. These activities ensure vital cellular processes such as nucleotide biosynthesis and lipid metabolism proceed smoothly.

ABCD4 mutations link to inborn errors like methylmalonic aciduria and vitamin B12 deficiency anemia. These conditions arise from impaired cobalamin processing due to dysfunctional transport activity. In particular during B12 deficiency anemia ABCD4’s interaction with vitamin B12 transport proteins such as LMBD1 results in inadequate vitamin transport leading to disrupted hematopoietic function and metabolic imbalances.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lysosomal membrane protein that transports cobalamin (Vitamin B12) from the lysosomal lumen to the cytosol in an ATP-dependent manner (PubMed : 22922874, PubMed : 28572511, PubMed : 31467407, PubMed : 33845046). Targeted by LMBRD1 lysosomal chaperone from the endoplasmic reticulum to the lysosomal membrane (PubMed : 27456980). Then forms a complex with lysosomal chaperone LMBRD1 and cytosolic MMACHC to transport cobalamin across the lysosomal membrane (PubMed : 25535791).
See full target information ABCD4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 14: PubMed39334940

2024

Connexin Expression Is Altered in the Eye Development of Mice: A Preliminary Study.

Applications

Unspecified application

Species

Unspecified reactive species

Ljubica Skelin,Anita Racetin,Nela Kelam,Marin Ogorevc,Ljubo Znaor,Mirna Saraga-Babić,Natalija Filipović,Yu Katsuyama,Zenon Pogorelić,Katarina Vukojević
View all publications
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