Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free

8 product images

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  Immunoprecipitation - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)

  This data was developed using ab250835, the same antibody clone in a different buffer formulation.
  Purified ab250835 at 1:60 dilution (2μg) immunoprecipitating ABCE1 in HEK-293 whole cell lysate.
  Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10 ?g
  Lane 2 (+): ab250835 + HEK-293 whole cell lysate.
  Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ABCE1 antibody [EPR15373(B)] - C-terminal ab185548 in HEK-293 whole cell lysate.
  VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) (1:1000 dilution) was used for Western blotting.
  Blocking Buffer and concentration: 5% NFDM/TBST.
  Diluting buffer and concentration: 5% NFDM/TBST.
  
  

  All lanes: Immunoprecipitation - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (Anti-ABCE1 antibody [EPR15373(B)] - C-terminal ab185548)

  Predicted band size: 67 kDa

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  Western blot - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)

  All lanes: Western blot - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (Anti-ABCE1 antibody [EPR15373(B)] - C-terminal ab185548) at 1/5000 dilution

  Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

  Lane 2: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate

  Lane 3: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

  Lane 4: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

  Lane 5: PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Predicted band size: 67 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)

  This data was developed using Anti-ABCE1 antibody [EPR15373(B)] - C-terminal ab185548, the same antibody clone in a different buffer formulation.
  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling ABCE1 with Purified Anti-ABCE1 antibody [EPR15373(B)] - C-terminal ab185548 at 1:300 (3.67 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

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  Flow Cytometry (Intracellular) - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)

  This data was developed using ab250835, the same antibody clone in a different buffer formulation.
  Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labelling ABCE1 with Purified ab250835 at 1:100 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

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  Immunocytochemistry/ Immunofluorescence - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)

  This data was developed using ab250835, the same antibody clone in a different buffer formulation.
  Immunocytochemistry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling ABCE1 with Purified ab250835 at 1:100 dilution (10 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A]+H21:L21 - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)

  This data was developed using Anti-ABCE1 antibody [EPR15373(B)] - C-terminal ab185548, the same antibody clone in a different buffer formulation.
  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling ABCE1 with Purified Anti-ABCE1 antibody [EPR15373(B)] - C-terminal ab185548 at 1:100 (11 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)

  This data was developed using Anti-ABCE1 antibody [EPR15373(B)] - C-terminal ab185548, the same antibody clone in a different buffer formulation.
  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling ABCE1 with Purified Anti-ABCE1 antibody [EPR15373(B)] - C-terminal ab185548 at 1:300 (3.67 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)

  This data was developed using Anti-ABCE1 antibody [EPR15373(B)] - C-terminal ab185548, the same antibody clone in a different buffer formulation.
  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling ABCE1 with Purified Anti-ABCE1 antibody [EPR15373(B)] - C-terminal ab185548 at 1:100 (11 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.