Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)

Rabbit Recombinant Monoclonal ABCE1 antibody. C-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 10 publications.

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Images

Immunoprecipitation - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (AB185548), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Expected	Tested
Rat	Expected	Tested	Expected	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30 - 1/50	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000 - 1/10000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/1000 - 1/10000	Notes -
Species Rat	Dilution info 1/1000 - 1/10000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/250	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/220	Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/100	Notes The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information ABCE1

Function

Nucleoside-triphosphatase (NTPase) involved in ribosome recycling by mediating ribosome disassembly (PubMed:20122402, PubMed:21448132). Able to hydrolyze ATP, GTP, UTP and CTP (PubMed:20122402). Splits ribosomes into free 60S subunits and tRNA- and mRNA-bound 40S subunits (PubMed:20122402, PubMed:21448132). Acts either after canonical termination facilitated by release factors (ETF1/eRF1) or after recognition of stalled and vacant ribosomes by mRNA surveillance factors (PELO/Pelota) (PubMed:20122402, PubMed:21448132). Involved in the No-Go Decay (NGD) pathway: recruited to stalled ribosomes by the Pelota-HBS1L complex, and drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway (PubMed:21448132). Also plays a role in quality control of translation of mitochondrial outer membrane-localized mRNA (PubMed:29861391). As part of the PINK1-regulated signaling, ubiquitinated by CNOT4 upon mitochondria damage; this modification generates polyubiquitin signals that recruit autophagy receptors to the mitochondrial outer membrane and initiate mitophagy (PubMed:29861391). RNASEL-specific protein inhibitor which antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) to RNASEL (PubMed:9660177). Negative regulator of the anti-viral effect of the interferon-regulated 2-5A/RNASEL pathway (PubMed:11585831, PubMed:9660177, PubMed:9847332). (Microbial infection) May act as a chaperone for post-translational events during HIV-1 capsid assembly. (Microbial infection) Plays a role in the down-regulation of the 2-5A/RNASEL pathway during encephalomyocarditis virus (EMCV) and HIV-1 infections.

Alternative names

RLI, RNASEL1, RNASELI, RNS4I, OK/SW-cl.40, ABCE1, ATP-binding cassette sub-family E member 1, 2'-5'-oligoadenylate-binding protein, HuHP68, RNase L inhibitor, Ribonuclease 4 inhibitor

Recommended products

Rabbit Recombinant Monoclonal ABCE1 antibody. C-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 10 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR15373(B)
Purification technique: Affinity purification Protein A
Specificity: The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ABCE1 also known as ATP-binding cassette sub-family E member 1 is an ATPase involved in various cellular processes. It belongs to the ATP-binding cassette (ABC) transporter family. The protein mass is approximately 68 kDa and its expression occurs widely in various tissues including the cytoplasm and mitochondria. ABCE1 has a unique structure with two nucleotide-binding domains making it distinct from other ABC family members that typically transport molecules across membranes.

Biological function summary

ABCE1 plays a role in inhibiting ribonuclease activity and is essential in ribosome recycling. This protein interacts with translation initiation factors and actively participates in the dissociation of ribosomes after translation termination. It is a part of the ribosome recycling complex an important multi-proteins setup necessary to prepare ribosomes for another round of translation. The biochemical interactions and presence in different cellular compartments highlight its multiple roles in cellular homeostasis.

Pathways

ABCE1 engages significantly in translation and its regulation. It plays an important role in the translation termination pathway where it interacts with eukaryotic release factors. Additionally it influences the degradation pathway of defective ribosomal products. Within these pathways the function of ABCE1 is closely linked to proteins like eRF1 and eRF3 which are essential in the translation termination process. The interaction of these proteins ensures efficient termination and recycling of translation machinery components.

Associated diseases and disorders

Alterations in ABCE1 expression or function link to certain cancers and viral infections. The aberrant activity of ABCE1 influences cancer progression as it affects protein synthesis and cellular growth levels. Also ABCE1 has been implicated in promoting the replication of certain viruses providing them a competitive advantage. Proteins like eIF3 and eIF4 involved in translation initiation can show altered associations in disorders where ABCE1 is dysregulated influencing disease outcomes and progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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9 product images

Immunoprecipitation - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
Purified ab185548 at 1:60 dilution (2μg) immunoprecipitating ABCE1 in HEK-293 whole cell lysate.
Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10 μg.
Lane 2 (+): ab185548 + HEK-293 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab185548 in HEK-293 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) (1:1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.

All lanes: Immunoprecipitation - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
Predicted band size: 67 kDa
Western blot - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
All lanes: Western blot - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548) at 1/5000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 4: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 5: PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 67 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling ABCE1 with Purified ab185548 at 1:300 (3.67 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Flow Cytometry (Intracellular) - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labelling ABCE1 with Purified ab185548 at 1:100 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
Immunocytochemistry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling ABCE1 with Purified ab185548 at 1:100 dilution (10 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A]+H21:L21 - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling ABCE1 with Purified ab185548 at 1:100 (11 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling ABCE1 with Purified ab185548 at 1:300 (3.67 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling ABCE1 with Purified ab185548 at 1:100 (11 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
ABCE1 western blot using anti-ABCE1 antibody [EPR15373(B)] - C-terminal ab185548. Publication image and figure legend from Yin, H., Jiang, Z., et al., 2019, Cell Death Dis, PubMed 31501431.

ab185548 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab185548 please see the product overview.
Upregulation of RNase L by IFN-γ neutralized RLI and restored cleavage activity.a, b Dose dependence (a) and time course (b) of IFN-γ in restoring the cleavage activity of RNase L (n = 3). c Cleavage activity of RNase L in the presence of IFN-γ in BEAS-2B and NCI-H157 cells (n = 3). d Western blot of OAS1, OAS2, and OAS3 in the presence of IFN-γ in BEAS-2B and NCI-H157 cells. e, f Western blot verification and rRNA cleavage after OAS3 interference by siRNA; g, j Western blot of RNase L dimer or monomer in the cytoplasm (g) or nucleus (j) in the presence of IFN-γ in BEAS-2B and NCI-H157 cells. h, i, k, l Cytoplasmic (h, i) and nuclear (k, l) RLI expression (i, l) and IP with RNase L (h, k) in the presence of IFN-γ in BEAS-2B and NCI-H157 cells