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AB50976

Anti-ABCF1 antibody [ABC5H06]

4

(1 Review)

|

(1 Publication)

Mouse Monoclonal ABCF1 antibody ABC5H06. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ABC50, ABCF1, ATP-binding cassette sub-family F member 1, ATP-binding cassette 50, TNF-alpha-stimulated ABC protein

1 Images
Western blot - Anti-ABCF1 antibody [ABC5H06] (AB50976)
  • WB

Unknown

Western blot - Anti-ABCF1 antibody [ABC5H06] (AB50976)

All lanes:

Western blot - Anti-ABCF1 antibody [ABC5H06] (ab50976) at 1/100 dilution

All lanes:

RIPA lysate of HeLa cells at 500 µg

Secondary

All lanes:

Anti-mouse IgG at 1/1000 dilution

Predicted band size: 95 kDa

Observed band size: 85 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ABC5H06

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human ABCF1. The exact immunogen used to generate this antibody is proprietary information.

Q8NE71

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Purification notes
Concentrated and dialyzed culture medium (protein free medium) of hybridoma
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: 1.16% Sodium chloride, 1% BSA, 0.316% Tris HCl
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCF1 also known as ATP-binding cassette sub-family F member 1 helps in cellular processes by facilitating the transport of various molecules across membranes. It weighs approximately 95 kDa. ABCF1 expression occurs in various tissues notably present in immune cells such as macrophages and monocytes. This protein does not function in drug transport and is an exception among ABC transporters because it lacks a transmembrane domain.
Biological function summary

ABCF1 plays a role in regulatory processes associated with immunity and inflammation. It acts as a critical component of the protein synthesis machinery influencing translation initiation. Although it does not form stable complexes it interacts transiently with ribosomal machinery. Its involvement in inflammation suggests it contributes to macrophage activation and innate immune responses.

Pathways

ABCF1 participates in inflammatory pathways and protein synthesis regulation. In the inflammatory pathway it engages in processes that modulate cytokine production. Also ABCF1 interfaces with proteins involved in translation like eukaryotic initiation factors regulating their activity during stress or immune response. These interactions make it a significant contributor to cellular protein homeostasis during inflammation.

ABCF1 connects to autoimmune conditions like rheumatoid arthritis. Increased expression in certain conditions implies its role in chronic inflammation. ABCF1 also interacts with TNF-alpha a cytokine heavily involved in inflammatory diseases suggesting its involvement in exacerbating inflammatory processes. Understanding ABCF1's regulatory mechanisms may aid in developing therapeutic interventions targeting inflammatory diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
See full target information ABCF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Physiological genomics 43:1065-73 PubMed21750230

2011

Impact of aging vs. estrogen loss on cardiac gene expression: estrogen replacement and inflammation.

Applications

WB

Species

Rat

Angela S Pechenino,Li Lin,Fiona N Mbai,Alison R Lee,Xian-Min He,John N Stallone,A A Knowlton
View all publications
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Product promise

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