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Rabbit Recombinant Monoclonal ABCF1 antibody. C-terminal. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Rat samples.

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Images

Western blot - Anti-ABCF1 antibody [EPR16068] - C-terminal (AB190798), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ABCF1 antibody [EPR16068] - C-terminal (AB190798), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ABCF1 antibody [EPR16068] - C-terminal (AB190798), expandable thumbnail
  • Western blot - Anti-ABCF1 antibody [EPR16068] - C-terminal (AB190798), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCF1 antibody [EPR16068] - C-terminal (AB190798), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested
Rat
Expected
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/10000 - 1/50000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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Target data

Function

Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.

Alternative names

ATP-binding cassette sub-family F member 1, ATP-binding cassette 50, TNF-alpha-stimulated ABC protein, ABC50, ABCF1

Recommended products

Rabbit Recombinant Monoclonal ABCF1 antibody. C-terminal. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Rat samples.

Alternative names

ATP-binding cassette sub-family F member 1, ATP-binding cassette 50, TNF-alpha-stimulated ABC protein, ABC50, ABCF1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR16068

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798), expandable thumbnail

    Western blot - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)

    All lanes: Western blot - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798) at 1/10000 dilution

    Lane 1: HeLa cell lysate at 20 µg

    Lane 2: K562 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 95 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling ABCF1 with ab190798 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue. Negative controls also shown.

  • Immunocytochemistry/ Immunofluorescence - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling ABCF1 with ab190798 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue. Negative controls also shown.

  • Western blot - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798), expandable thumbnail

    Western blot - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)

    All lanes: Western blot - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798) at 1/50000 dilution

    All lanes: 293T (Human embryonic kidney epithelial cell) cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 95 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ABCF1 with ab190798 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)

    Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling ABCF1 with ab190798 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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