AB190798

Anti-ABCF1 antibody [EPR16068] - C-terminal

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal ABCF1 antibody. C-terminal. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Rat samples. Cited in 2 publications.

View Alternative Names

ABC50, ABCF1, ATP-binding cassette sub-family F member 1, ATP-binding cassette 50, TNF-alpha-stimulated ABC protein

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-ABCF1 antibody [EPR16068] - C-terminal (AB190798)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ABCF1 antibody [EPR16068] - C-terminal (AB190798)

Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling ABCF1 with ab190798 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue. Negative controls also shown.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCF1 antibody [EPR16068] - C-terminal (AB190798)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCF1 antibody [EPR16068] - C-terminal (AB190798)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ABCF1 with ab190798 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ABCF1 antibody [EPR16068] - C-terminal (AB190798)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling ABCF1 with ab190798 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue. Negative controls also shown.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCF1 antibody [EPR16068] - C-terminal (AB190798)

Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling ABCF1 with ab190798 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-ABCF1 antibody [EPR16068] - C-terminal (AB190798)

All lanes:

Western blot - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798) at 1/50000 dilution

All lanes:

293T (Human embryonic kidney epithelial cell) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 95 kDa

false

Supplier Data

Western blot - Anti-ABCF1 antibody [EPR16068] - C-terminal (AB190798)

All lanes:

Western blot - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 95 kDa

false

Carrier free

Anti-ABCF1 antibody [EPR16068] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16068

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCF1 also known as ATP-binding cassette sub-family F member 1 helps in cellular processes by facilitating the transport of various molecules across membranes. It weighs approximately 95 kDa. ABCF1 expression occurs in various tissues notably present in immune cells such as macrophages and monocytes. This protein does not function in drug transport and is an exception among ABC transporters because it lacks a transmembrane domain.

Biological function summary

ABCF1 plays a role in regulatory processes associated with immunity and inflammation. It acts as a critical component of the protein synthesis machinery influencing translation initiation. Although it does not form stable complexes it interacts transiently with ribosomal machinery. Its involvement in inflammation suggests it contributes to macrophage activation and innate immune responses.

Pathways

ABCF1 participates in inflammatory pathways and protein synthesis regulation. In the inflammatory pathway it engages in processes that modulate cytokine production. Also ABCF1 interfaces with proteins involved in translation like eukaryotic initiation factors regulating their activity during stress or immune response. These interactions make it a significant contributor to cellular protein homeostasis during inflammation.

ABCF1 connects to autoimmune conditions like rheumatoid arthritis. Increased expression in certain conditions implies its role in chronic inflammation. ABCF1 also interacts with TNF-alpha a cytokine heavily involved in inflammatory diseases suggesting its involvement in exacerbating inflammatory processes. Understanding ABCF1's regulatory mechanisms may aid in developing therapeutic interventions targeting inflammatory diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.

See full target information ABCF1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 41:130 PubMed40936027

2025

SP1 and p23 play a crucial role in the circadian target gene induction of activated aryl hydrocarbon receptor in human breast cells.

Applications

Unspecified application

Species

Unspecified reactive species

Melina Mihelakis,Tanina Flore,Gilbert Schönfelder,Michael Oelgeschläger,Norman Ertych

Developmental neuroscience 46:210-220 PubMed37757768

2023

ABCF1/CXCL12/CXCR4 Enhances Glioblastoma Cell Proliferation, Migration, and Invasion by Activating the PI3K/AKT Signal Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohong Yin,Keshun Xia,Song Peng,Bo Tan,Yaohui Huang,Mao Wang,Mingfang He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ABCF1 antibody [EPR16068] - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-ABCF1 antibody [EPR16068] - C-terminal (AB190798)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCF1 antibody [EPR16068] - C-terminal (AB190798)

Immunocytochemistry/ Immunofluorescence - Anti-ABCF1 antibody [EPR16068] - C-terminal (AB190798)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCF1 antibody [EPR16068] - C-terminal (AB190798)

Western blot - Anti-ABCF1 antibody [EPR16068] - C-terminal (AB190798)

Western blot - Anti-ABCF1 antibody [EPR16068] - C-terminal (AB190798)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise