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AB50807

Anti-ABCF2 antibody [2001C1]

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(1 Publication)

Mouse Monoclonal ABCF2 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ABCF2.

View Alternative Names

HUSSY-18, ABCF2, ATP-binding cassette sub-family F member 2, Iron-inhibited ABC transporter 2

2 Images
Western blot - Anti-ABCF2 antibody [2001C1] (AB50807)
  • WB

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Western blot - Anti-ABCF2 antibody [2001C1] (AB50807)

Lanes 1-3 : Merged signal (red and green). Green - ab50807 observed at 75 kDa. Red - loading control ab181602 observed at 36 kDa.

ab50807 Anti-ABCF2 antibody [2001C1] was shown to specifically react with ABCF2 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266516 (knockout cell lysate ab258773) was used. Wild-type and ABCF2 knockout samples were subjected to SDS-PAGE. ab50807 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ABCF2 antibody [2001C1] (ab50807) at 1/500 dilution

Lane 1:

Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

ABCF2 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

Western blot - Human ABCF2 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-abcf2-knockout-hek-293t-cell-line-ab266516'>ab266516</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/10000 dilution

Predicted band size: 71 kDa

Observed band size: 75 kDa

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Western blot - Anti-ABCF2 antibody [2001C1] (AB50807)
  • WB

Unknown

Western blot - Anti-ABCF2 antibody [2001C1] (AB50807)

All lanes:

Western blot - Anti-ABCF2 antibody [2001C1] (ab50807)

All lanes:

Recombinant ABCF2 fragment

Predicted band size: 71 kDa

Observed band size: 33 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2001C1

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ABCF2.

Q9UG63

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
ab50807 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The human ABCF2 protein also known as ATP-binding cassette sub-family F member 2 belongs to the ABC transporter superfamily. Weighing approximately 85 kDa ABCF2 does not transport molecules across membranes like most other members of this family. Instead it participates in regulating translation and is involved in cellular stress responses. Researchers find ABCF2 expression in various tissues including liver lung and muscle. It does not span membranes and it anchors within the cytoplasm of cells.
Biological function summary

ABCF2 impacts translation regulation and stress response through different cellular processes. This protein functions independently and may also participate in complexes with other proteins. Through mechanisms not fully understood ABCF2 associates with cellular stress granules that form in response to environmental stress. These interactions suggest roles in protein synthesis regulation during stress which indirectly influences cell survival and adaptation.

Pathways

ABCF2 integrates into cellular processes related to stress and translation. It intersects with pathways like eukaryotic initiation factor 2 (EIF2) signaling which helps control translation initiation during stress. ABCF2 shares pathway interactions with proteins such as EIF2B and other ribosomal elements indicating a connection to translation regulation. The involvement in ribosomal function places ABCF2 within important cellular response networks especially under conditions that affect protein synthesis fidelity.

ABCF2 links to specific pathologies primarily through stress response mechanisms. Cancer particularly hepatic and pulmonary types shows associations with dysregulated ABCF2 expression possibly affecting tumor growth and chemoresistance. Researchers note potential interactions with heat shock proteins which share pathways implicated in cancer cell survival. Additionally preliminary evidence suggests ABCF2 roles in neurological disorders although the connections and interactions with other proteins need further study.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information ABCF2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical cancer research : an official journal of the American Association for Cancer Research 21:3512-21 PubMed26240291

2015

Identification of a Three-Biomarker Panel in Urine for Early Detection of Pancreatic Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tomasz P Radon,Nathalie J Massat,Richard Jones,Wasfi Alrawashdeh,Laurent Dumartin,Darren Ennis,Stephen W Duffy,Hemant M Kocher,Stephen P Pereira,Luisa Guarner posthumous,Cristiane Murta-Nascimento,Francisco X Real,Núria Malats,John Neoptolemos,Eithne Costello,William Greenhalf,Nick R Lemoine,Tatjana Crnogorac-Jurcevic
View all publications
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