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AB113953

Anti-ABCF3 antibody

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Rabbit Polyclonal ABCF3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human ABCF3 aa 650 to C-terminus.

View Alternative Names

ATP-binding cassette sub-family F member 3, ABCF3

2 Images
Immunoprecipitation - Anti-ABCF3 antibody (AB113953)
  • IP

Unknown

Immunoprecipitation - Anti-ABCF3 antibody (AB113953)

Detection of ABCF3 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab113953 at 3 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-ABCF3 antibody (ab113953)

Predicted band size: 80 kDa

false

Western blot - Anti-ABCF3 antibody (AB113953)
  • WB

Unknown

Western blot - Anti-ABCF3 antibody (AB113953)

All lanes:

Western blot - Anti-ABCF3 antibody (ab113953) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 80 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ABCF3 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NUQ8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ABCF3 protein also known as ATP-binding cassette sub-family F member 3 functions in cellular processes involving ATP binding and hydrolysis. This protein weighs approximately 95 kDa. It shows expression in several tissues including the liver kidney and testis. As part of the ATP-binding cassette (ABC) family it lacks a transmembrane domain but participates significantly in nucleotide binding.
Biological function summary

ABCF3 plays a role in cellular stress response and protein synthesis regulation. It often forms part of a multi-protein complex aiding the translation process by interacting with ribosomal subunits. This interaction suggests an involvement in the regulation of translation initiation which is important for effective protein synthesis. Additionally ABCF3 may have a role in maintaining cellular homeostasis under stress conditions.

Pathways

The ABCF3 protein significantly contributes to the mechanistic target of rapamycin (mTOR) signaling pathway and the integrated stress response pathway. Its interaction with ribosomal proteins suggests a regulatory effect on mTOR signaling which is integral to cell growth and metabolism. ABCF3 also indirectly influences other signaling proteins involved in these pathways such as eukaryotic initiation factors which are key translational regulators.

Studies show ABCF3's involvement in cancer and inflammatory responses. Aberrant expression of ABCF3 links with tumorigenesis influencing cancer cell proliferation and survival. It connects to other cancer-related proteins including mTOR-related proteins highlighting its role in tumor progression. Additionally involvement in inflammatory pathways suggests connections to inflammation-driven conditions where ABCF3 might affect inflammatory regulators.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Displays an antiviral effect against flaviviruses such as west Nile virus (WNV) in the presence of OAS1B.
See full target information ABCF3
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Product promise

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