AB52617

Anti-ABCG1 antibody [EP1366Y]

Name: Anti-ABCG1 antibody [EP1366Y] (ab52617) | Abcam
Brand: Abcam Limited
SKU: AB52617
Price: 635 USD
Availability: InStock
Rating: 4 (5 reviews)

RabMAb
Recombinant
What is this?

(5 Reviews)

(105 Publications)

Anti-ABCG1 antibody [EP1366Y] (ab52617) is a rabbit monoclonal antibody detecting ABCG1 in Western Blot, ICC/IF. Suitable for Cow, Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 80 publications
- Trusted since 2007

View Alternative Names

ABC8, WHT1, ABCG1, ATP-binding cassette sub-family G member 1, ATP-binding cassette transporter 8, White protein homolog

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] (AB52617)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] (AB52617)

ab52617 staining ABCG1 in HUVEC (Human umbilical vein endothelial cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab52617 at 1/200. DAPI was used as a nuclear counterstain and PBS as a negative control.

Lab

Western blot - Anti-ABCG1 antibody [EP1366Y] (AB52617)

Blocking and diluting buffer : 5% NFDM /TBST

All lanes:

Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617) at 1/1000 dilution

Lane 1:

Rat kidney lysate at 20 µg

Lane 2:

Rat liver lysate at 20 µg

Lane 3:

MDBK (Bovine kidney cell line) whole cell lysate at 20 µg

Lane 4:

HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 20 µg

Lane 5:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 6:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 75 kDa

false

Supplier Data

Western blot - Anti-ABCG1 antibody [EP1366Y] (AB52617)

Blocking/Dilution buffer : 5% NFDM/TBST

Lane 1:

Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617) at 1/1000 dilution

Lane 2:

Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (<a href='/en-us/products/primary-antibodies/ddddk-tag-binds-to-flag-tag-sequence-antibody-epr20018-251-ab205606'>ab205606</a>) at 1/1000 dilution

All lanes:

C-Myc/DDK-tagged full length human ABCG1 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 180s

Unknown

Western blot - Anti-ABCG1 antibody [EP1366Y] (AB52617)

All lanes:

Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617) at 1/2000 dilution

All lanes:

HUVEC cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP antiibody at 1/2000 dilution

Predicted band size: 75 kDa

Observed band size: 110 kDa

false

HRP

HRP Anti-ABCG1 antibody [EP1366Y]
Carrier free

Anti-ABCG1 antibody [EP1366Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1366Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Cow, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-ABCG1 antibody [EP1366Y] (ab52617) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Cow, Human, Mouse, Rat samples.

What is the molecular weight of ABCG1?
Anti-ABCG1 [EP1366Y] (ab52617) specifically detects a band for ABCG1 (UniProt: P45844) at a molecular weight of 75kDa.

Trusted by the scientific community
Anti-ABCG1 [EP1366Y] (ab52617) was first used in a scientific publication in 2007 and has been cited over 80 times in peer-reviewed journals.

Reviewed by scientists
Anti-ABCG1 [EP1366Y] (ab52617) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EP1366Y] also available for your convenience: ab52617, HRP - ab201776, Carrier free - ab218528

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCG1 also known as ATP-binding cassette sub-family G member 1 functions as a cholesterol transporter in cells. The protein facilitates the efflux of cholesterol to high-density lipoproteins (HDL). With a molecular mass of approximately 75 kDa ABCG1 is located in many tissues but its expression levels are highest in macrophages and endothelial cells. As a member of the ABC transporter family it plays a critical role in cellular lipid homeostasis.

Biological function summary

ABCG1 works to regulate cholesterol levels by exporting excess cholesterol and phospholipids. This protein does not operate in isolation; it often interacts with other proteins forming heterodimers especially with ABCG4. These interactions contribute to its role in maintaining lipid metabolism by facilitating efficient cholesterol clearance improving cellular lipid balance and reducing intracellular lipid toxicity.

Pathways

ABCG1 plays an important role in the reverse cholesterol transport pathway and is involved in lipid metabolism. In the context of these pathways ABCG1 interacts with proteins like LXR (liver X receptor) which regulates its expression. LXRs when activated by oxysterols can influence ABCG1 activity promoting cholesterol efflux to HDL and contributing to overall cholesterol homeostasis in the body.

ABCG1 has connections to atherosclerosis and metabolic syndrome. Disruption in ABCG1 function or expression can lead to impaired cholesterol efflux contributing to lipid accumulation in macrophages and formation of foam cells an important event in atherosclerosis development. In this disease context ABCG1 interacts with proteins like ApoA-I and ApoA-II which are components of HDL and are essential for efficient cholesterol transport to the liver highlighting its critical role in cardiovascular health management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the efflux of phospholipids such as sphingomyelin, cholesterol and its oxygenated derivatives like 7beta-hydroxycholesterol and this transport is coupled to hydrolysis of ATP (PubMed : 17408620, PubMed : 24576892). The lipid efflux is ALB-dependent (PubMed : 16702602). Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol (PubMed : 17408620).

See full target information ABCG1

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Publications (105)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 15: PubMed40867523

2025

Artesunate Ameliorates SLE Atherosclerosis Through PPARγ-Driven Cholesterol Efflux Restoration and Disruption of Lipid Raft-Organized TLR9/MyD88 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Zhang,Xinyu Pan,Yuanfang He,Kairong Sun,Zhiyu Wang,Weiyu Tian,Haonan Qiu,Yiqi Wang,Chengping Wen,Juan Chen

Molecular medicine (Cambridge, Mass.) 31:99 PubMed40087552

2025

LXRα agonists ameliorates acute rejection after liver transplantation via ABCA1/MAPK and PI3K/AKT/mTOR signaling axis in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Qin,Dingheng Hu,Qi Li,Shiyi Zhang,Zheng Qin,Liangxu Wang,Rui Liao,Zhongjun Wu,Yanyao Liu

International journal of chronic obstructive pulmonary disease 20:411-423 PubMed40008109

2025

Cholesterol Accumulation Enhances Cigarette Smoke-Induced Airway Epithelial Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Du Jing,Jin-Kang Yu,Hai-Pin Chen,Ling-Ling Dong,Wen Li,Zhou-Yang Li,Jie-Sen Zhou

Histology and histopathology 40:585-596 PubMed39315497

2024

The Lian-Dou-Qing-Mai Formula activates the PPARγ-LXRα-ABCA1/ABCG1 pathway by regulating IL-10, leading to the promotion of cholesterol efflux and a reduction in atherosclerotic plaques.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqi Liao,You Li,Haoyan Zhao,Shu Lu

iScience 27:109849 PubMed38784008

2024

TUDCA alleviates atherosclerosis by inhibiting AIM2 inflammasome and enhancing cholesterol efflux capacity in macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Xuyang Wang,Yuesheng Zhang,Luping Du,Zhengchen Jiang,Yan Guo,Kai Wang,Yijiang Zhou,Xiang Yin,Xiaogang Guo

The Journal of clinical investigation 134: PubMed38747290

2024

LXR signaling pathways link cholesterol metabolism with risk for prediabetes and diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Jingzhong Ding,Anh Tram Nguyen,Kurt Lohman,Michael T Hensley,Daniel Parker,Li Hou,Jackson Taylor,Deepak Voora,Janet K Sawyer,Elena Boudyguina,Michael P Bancks,Alain Bertoni,James S Pankow,Jerome I Rotter,Mark O Goodarzi,Russell P Tracy,David M Murdoch,Daniel Duprez,Stephen S Rich,Bruce M Psaty,David Siscovick,Christopher Newgard,David Herrington,Ina Hoeschele,Steven Shea,James H Stein,Manesh Patel,Wendy Post,David Jacobs,John S Parks,Yongmei Liu

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e23522 PubMed38445789

2024

Microsomal triglyceride transfer protein is necessary to maintain lipid homeostasis and retinal function.

Applications

Unspecified application

Species

Unspecified reactive species

Catharina R Grubaugh,Anuradha Dhingra,Binu Prakash,Diego Montenegro,Janet R Sparrow,Lauren L Daniele,Christine A Curcio,Brent A Bell,M Mahmood Hussain,Kathleen Boesze-Battaglia

Aging 15:14791-14802 PubMed38157252

2024

IGFBPL1 inhibits macrophage lipid accumulation by enhancing the activation of IGR1R/LXRα/ABCG1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lianjie Hou,Xixi Feng,Zhi Zhu,Yali Mi,Qin He,Kai Yin,Guojun Zhao

Cell death & disease 14:542 PubMed37607939

2023

Smooth muscle liver kinase B1 inhibits foam cell formation and atherosclerosis via direct phosphorylation and activation of SIRT6.

Applications

Unspecified application

Species

Unspecified reactive species

Qiming Deng,Hongxuan Li,Xiaolin Yue,Chenghu Guo,Yuanyuan Sun,Chang Ma,Jiangang Gao,Yue Wu,Bin Du,Jianmin Yang,Cheng Zhang,Wencheng Zhang

Stem cell research & therapy 14:214 PubMed37605285

2023

APOE deficiency impacts neural differentiation and cholesterol biosynthesis in human iPSC-derived cerebral organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhao,Tadafumi C Ikezu,Wenyan Lu,Jesse R Macyczko,Yonghe Li,Laura J Lewis-Tuffin,Yuka A Martens,Yingxue Ren,Yiyang Zhu,Yan W Asmann,Nilüfer Ertekin-Taner,Takahisa Kanekiyo,Guojun Bu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ABCG1 antibody [EP1366Y]

Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] (AB52617)

Western blot - Anti-ABCG1 antibody [EP1366Y] (AB52617)

Western blot - Anti-ABCG1 antibody [EP1366Y] (AB52617)

Western blot - Anti-ABCG1 antibody [EP1366Y] (AB52617)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (105)

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