JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB218528

Anti-ABCG1 antibody [EP1366Y] - BSA and Azide free

Be the first to review this product! Submit a review

|

(18 Publications)

Rabbit Recombinant Monoclonal ABCG1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human, Cow samples. Cited in 18 publications.

View Alternative Names

ABC8, WHT1, ABCG1, ATP-binding cassette sub-family G member 1, ATP-binding cassette transporter 8, White protein homolog

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] - BSA and Azide free (AB218528)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] - BSA and Azide free (AB218528)

ab52617 staining ABCG1 in HUVEC (Human umbilical vein endothelial cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab52617 at 1/200. DAPI was used as a nuclear counterstain and PBS as a negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52617).

Western blot - Anti-ABCG1 antibody [EP1366Y] - BSA and Azide free (AB218528)
  • WB

Supplier Data

Western blot - Anti-ABCG1 antibody [EP1366Y] - BSA and Azide free (AB218528)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52617). Blocking/Dilution buffer : 5% NFDM/TBST

Lane 1:

Western blot - Anti-ABCG1 antibody [EP1366Y] (<a href='/en-us/products/primary-antibodies/abcg1-antibody-ep1366y-ab52617'>ab52617</a>) at 1/1000 dilution

Lane 2:

Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (<a href='/en-us/products/primary-antibodies/ddddk-tag-binds-to-flag-tag-sequence-antibody-epr20018-251-ab205606'>ab205606</a>) at 1/1000 dilution

All lanes:

C-Myc/DDK-tagged full length human ABCG1 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 180s

  • Unconjugated

    Anti-ABCG1 antibody [EP1366Y]

  • HRP

    HRP Anti-ABCG1 antibody [EP1366Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1366Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Cow, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB244442

Anti-ABCG1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody (AB244442)

  • 0
  • 0
Primary Antibodies

AB217023

Anti-ABCG1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCG1 antibody (AB217023)

  • 0
  • 0

Complementary Products

Assay Kits

AB65390

Cholesterol Assay Kit - HDL and LDL/VLDL

Biochemical assay - Cholesterol Assay Kit - HDL and LDL/VLDL (AB65390)

  • 4
  • 10
Primary Antibodies

AB66217

Anti-ABCA1 antibody [HJ1]

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [HJ1] (AB66217)

  • 3
  • 3
Primary Antibodies

AB52629

Anti-Scavenging Receptor SR-BI antibody [EP1556Y]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (AB52629)

  • 5
  • 4
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB176323

Anti-LXR alpha antibody [EPR6508(N)]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-LXR alpha antibody [EPR6508(N)] (AB176323)

  • 4
  • 3
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

ab218528 is the carrier-free version of ab52617.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCG1 also known as ATP-binding cassette sub-family G member 1 functions as a cholesterol transporter in cells. The protein facilitates the efflux of cholesterol to high-density lipoproteins (HDL). With a molecular mass of approximately 75 kDa ABCG1 is located in many tissues but its expression levels are highest in macrophages and endothelial cells. As a member of the ABC transporter family it plays a critical role in cellular lipid homeostasis.
Biological function summary

ABCG1 works to regulate cholesterol levels by exporting excess cholesterol and phospholipids. This protein does not operate in isolation; it often interacts with other proteins forming heterodimers especially with ABCG4. These interactions contribute to its role in maintaining lipid metabolism by facilitating efficient cholesterol clearance improving cellular lipid balance and reducing intracellular lipid toxicity.

Pathways

ABCG1 plays an important role in the reverse cholesterol transport pathway and is involved in lipid metabolism. In the context of these pathways ABCG1 interacts with proteins like LXR (liver X receptor) which regulates its expression. LXRs when activated by oxysterols can influence ABCG1 activity promoting cholesterol efflux to HDL and contributing to overall cholesterol homeostasis in the body.

ABCG1 has connections to atherosclerosis and metabolic syndrome. Disruption in ABCG1 function or expression can lead to impaired cholesterol efflux contributing to lipid accumulation in macrophages and formation of foam cells an important event in atherosclerosis development. In this disease context ABCG1 interacts with proteins like ApoA-I and ApoA-II which are components of HDL and are essential for efficient cholesterol transport to the liver highlighting its critical role in cardiovascular health management.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the efflux of phospholipids such as sphingomyelin, cholesterol and its oxygenated derivatives like 7beta-hydroxycholesterol and this transport is coupled to hydrolysis of ATP (PubMed : 17408620, PubMed : 24576892). The lipid efflux is ALB-dependent (PubMed : 16702602). Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol (PubMed : 17408620).
See full target information ABCG1
style
left-column

Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 82:131 PubMed40137979

2025

Microglia regulate myelin clearance and cholesterol metabolism after demyelination via interferon regulatory factor 5.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandro Montilla,Alazne Zabala,Ibai Calvo,Marina Bosch-Juan,Irene Tomé-Velasco,Paloma Mata,Mirjam Koster,Amanda Sierra,Susanne M Kooistra,Federico N Soria,Bart J L Eggen,Olatz Fresnedo,José Andrés Fernández,Vanja Tepavcevic,Carlos Matute,María Domercq

Chinese medicine 19:5 PubMed38183139

2024

Combination of mangiferin and T0901317 targeting autophagy promotes cholesterol efflux from macrophage foam cell in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Chen,Sijian Wang,Ruixia Bao,Dan Wang,Yuzheng Wu,Yi Zhang,Mengyang Liu,Tao Wang

Technology in cancer research & treatment 20:15330338211039127 PubMed34747666

2021

MiR-495 Inhibits Cisplatin Resistance and Angiogenesis in Esophageal Cancer by Targeting ATP7A.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuanghua Li,Shaowen Li,Yongqin Wen,Jingtang Chen,Kejun Liu,Jun Jia

Oncogene 39:3754-3773 PubMed32157214

2020

Identification of Sca-1Abcg1 bronchioalveolar epithelial cells as the origin of lung adenocarcinoma in Gprc5a-knockout mouse model through the interaction between lung progenitor AT2 and Lgr5 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huijing Yin,Zhengyu Jiang,Xu Feng,Zhaodong Ji,Wei Jin

International journal of molecular medicine 44:2003-2014 PubMed31638178

2019

miR‑146a‑5p suppresses ATP‑binding cassette subfamily G member 1 dysregulation in patients with refractory Mycoplasma pneumoniae via interleukin 1 receptor‑associated kinase 1 downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Hu-Nian Li,Xu Zhao,Yong-Jiu Zha,Fang Du,Jie Liu,Liang Sun

Journal of molecular and cellular cardiology 79:287-94 PubMed25528964

2014

RIP140 contributes to foam cell formation and atherosclerosis by regulating cholesterol homeostasis in macrophages.

Applications

WB

Species

Mouse

Yi-Wei Lin,Pu-Ste Liu,Neeta Adhikari,Jennifer L Hall,Li-Na Wei

Journal of lipid research 55:681-97 PubMed24493833

2014

A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is essential for the regulation of cholesterol homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Wei Hu,Jun-Yao Yang,Xin Ma,Zhi-Ping Chen,Ya-Rong Hu,Jia-Yi Zhao,Shu-Fen Li,Yu-Rong Qiu,Jing-Bo Lu,Yan-Chao Wang,Ji-Juan Gao,Yan-Hua Sha,Lei Zheng,Qian Wang

Cell death & disease 4:e780 PubMed23990020

2013

Pro-apoptotic miRNA-128-2 modulates ABCA1, ABCG1 and RXRα expression and cholesterol homeostasis.

Applications

WB

Species

Human

Y K Adlakha,S Khanna,R Singh,V P Singh,A Agrawal,N Saini

The Journal of pharmacology and experimental thera 346:381-92 PubMed23820125

2013

The novel anticancer agent JNJ-26854165 induces cell death through inhibition of cholesterol transport and degradation of ABCA1.

Applications

Unspecified application

Species

Unspecified reactive species

Richard J Jones,Dongmin Gu,Chad C Bjorklund,Isere Kuiatse,Alan T Remaley,Tarig Bashir,Veronique Vreys,Robert Z Orlowski

The American journal of pathology 182:363-74 PubMed23219426

2012

Molecular profiling of prostatic acinar morphogenesis identifies PDCD4 and KLF6 as tissue architecture-specific prognostic markers in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chi-Rong Li,Jimmy J-M Su,Wei-Yu Wang,Michael T-L Lee,Ting-Yun Wang,Kuan-Ying Jiang,Chein-Feng Li,Jong-Ming Hsu,Chi-Kuan Chen,Marcelo Chen,Shih-Sheng Jiang,Valerie M Weaver,Kelvin K-C Tsai
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com