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AB214288

Anti-ABCG5 antibody

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Rabbit Polyclonal ABCG5 antibody. Suitable for IHC-P and reacts with Rat samples. Immunogen corresponding to Synthetic Peptide within Human ATP-binding cassette sub-family G member 5 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

ATP-binding cassette sub-family G member 5, Sterolin-1, ABCG5

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCG5 antibody (AB214288)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCG5 antibody (AB214288)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat liver tissue, labeling ABCG5 with ab214288 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human ATP-binding cassette sub-family G member 5 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9H222

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCG5 also known as ATP-binding cassette sub-family G member 5 is a transporter protein involved in lipid homeostasis. It has a molecular mass of approximately 73 kDa. ABCG5 is expressed mainly in the liver and intestine. This protein partners with ABCG8 to form a functional unit that helps mediate the transport of sterols across cell membranes specifically to facilitate the excretion of plant sterols and cholesterol from the body.
Biological function summary

The ABCG5 and ABCG8 complex plays a critical role in maintaining cholesterol balance and prevents the accumulation of sterols. Through its ability to regulate sterol excretion ABCG5 indirectly influences cholesterol levels in the bloodstream. By transporting sterols from enterocytes back into the intestinal lumen and from hepatocytes into bile it preserves the delicate balance between absorption and excretion of sterols.

Pathways

ABCG5 and its partner protein ABCG8 are integral to the sterol regulatory element-binding proteins (SREBP) pathway. This pathway is significant for cholesterol metabolism and homeostasis. The action of ABCG5 in this pathway coordinates with proteins such as NPC1L1 which is responsible for cholesterol absorption in the intestine to ensure efficient cholesterol trafficking and clearance.

ABCG5 mutations are closely related to sitosterolemia a rare inherited lipid disorder characterized by hyperabsorption of plant sterols and cholesterol. Alterations in ABCG5 function lead to increased plasma sterol levels and cholesterol deposits in tissues contributing to atherosclerosis. The interaction between ABCG5 and ABCG8 is important in understanding sitosterolemia as any dysfunction in this pairing disrupts the body's capacity to regulate sterol levels effectively.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane (PubMed : 27144356). Plays an essential role in the selective transport of dietary plant sterols and cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile (PubMed : 11099417, PubMed : 11138003, PubMed : 15054092, PubMed : 27144356). Required for normal sterol homeostasis (PubMed : 11099417, PubMed : 11138003, PubMed : 15054092). The heterodimer with ABCG8 has ATPase activity (PubMed : 16893193, PubMed : 20210363, PubMed : 27144356).
See full target information ATP-binding cassette sub-family G member 5
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