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AB126493

Anti-ABCG8 antibody

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(4 Publications)

Rabbit Polyclonal ABCG8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP-binding cassette sub-family G member 8 aa 250-450.

View Alternative Names

ATP-binding cassette sub-family G member 8, Sterolin-2, ABCG8

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-ABCG8 antibody (AB126493)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ABCG8 antibody (AB126493)

Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm. Recommended concentration of ab126493 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCG8 antibody (AB126493)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCG8 antibody (AB126493)

ab126493 at 1/50 dilution staining ABCG8 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.

Western blot - Anti-ABCG8 antibody (AB126493)
  • WB

Unknown

Western blot - Anti-ABCG8 antibody (AB126493)

All lanes:

Western blot - Anti-ABCG8 antibody (ab126493) at 1/250 dilution

Lane 1:

RT4 cell lysate

Lane 2:

U251 MG cell lysate

Lane 3:

Human plasma lysate

Lane 4:

Human liver tissue lysate

Lane 5:

Human tonsil tissue lysate

Predicted band size: 76 kDa

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Western blot - Anti-ABCG8 antibody (AB126493)
  • WB

CiteAb

Western blot - Anti-ABCG8 antibody (AB126493)

Western Blotting using Anti-ABCG8 antibody, ab126493. Publication image from Caruso, D. et al., 2017, Nat Commun, 28580927. Legend direct from paper.

ZA treatment does not affect other transporters in ZA and SIM-treated DC.Levels of the indicated plasma-membrane-associated proteins are evaluated by western blotting in biotinylated extracts from untreated or 1 µM ZA and/or simvastatin (SIM) treated DC. Pancadherin is employed as a control of equal protein loading. The blots are representative of one out of the three experiments. (b) Activities of the indicated plasma-membrane-associated proteins are measured after immunopurification from the biotinylated extracts of DC treated, as shown in a. The results are expressed as nmoles hydrolysed phosphate (Pi)/min/mg proteins according to the previously prepared titration curve. The NHE1 activities and SLC4A1 activities are fluorimetrically measured, and the results are expressed as δpHi/min and arbitrary units, respectively. The bars represent the mean±s.e.m. of three experiments. None of the differences is statistically significant.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ATP-binding cassette sub-family G member 8 aa 250-450. The exact immunogen used to generate this antibody is proprietary information.

Q9H221

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Recommend PFA Fixation and Triton X-100 treatment</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCG8 also known as ATP-binding cassette sub-family G member 8 is a transporter protein involved in cholesterol homeostasis. It is a part of the ATP-binding cassette (ABC) family with an approximate mass of 74 kDa. ABCG8 is expressed in the liver and small intestine where it plays a role in the export of sterols. This protein functions as a half-transporter and pairs with ABCG5 to form a functional heterodimer which is essential for its function in cellular settings.
Biological function summary

ATP-binding cassette members like ABCG8 participate directly in the movement of lipids across cellular membranes. This transporter is part of a heterodimer complex with ABCG5 that mediates the excretion of sterols into the bile and intestinal lumen. The ABCG5/ABCG8 complex ensures the removal of excess cholesterol and plant sterols from the body maintaining lipid balance and preventing sterol accumulation.

Pathways

The cholesterol transport functions of ABCG8 integrate into the hepatic and intestinal cholesterol efflux pathways. It interacts with proteins and processes in the reverse cholesterol transport pathway and impacts the rate of cholesterol clearance. The ABCG5/ABCG8 heterodimer works alongside proteins like LDL receptors playing a part in a broader network ensuring cholesterol homeostasis and absorption is maintained at optimal levels.

Mutations or malfunctions in ABCG8 link to conditions such as sitosterolemia and gallstone disease. Sitosterolemia is characterized by the accumulation of plant sterols due to impaired function of the transporter complex which affects ABCG5 similarly since they function together. Additionally altered activity of ABCG8 can influence cholesterol crystal formation leading to gallstone development by disrupting the normal biliary sterol composition.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile (PubMed : 11099417, PubMed : 11452359, PubMed : 15054092, PubMed : 27144356). Required for normal sterol homeostasis (PubMed : 11099417, PubMed : 11452359, PubMed : 15054092). The heterodimer with ABCG5 has ATPase activity (PubMed : 16893193, PubMed : 20210363, PubMed : 27144356).
See full target information ATP-binding cassette sub-family G member 8
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Foods (Basel, Switzerland) 10: PubMed34829135

2021

The Effects of Anthocyanin-Rich Bilberry Extract on Transintestinal Cholesterol Excretion.

Applications

Unspecified application

Species

Unspecified reactive species

Jimin Hong,Minji Kim,Bohkyung Kim

Lipids in health and disease 18:218 PubMed31829191

2019

Pioglitazone prevents cholesterol gallstone formation through the regulation of cholesterol homeostasis in guinea pigs with a lithogenic diet.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Han,Yangge Lv,Shijia Wang,Tao Hu,Hao Hong,Zan Fu

Cell metabolism 26:738-752.e6 PubMed28988824

2017

Citrobacter rodentium Subverts ATP Flux and Cholesterol Homeostasis in Intestinal Epithelial Cells In Vivo.

Applications

WB

Species

Unspecified reactive species

Cedric N Berger,Valerie F Crepin,Theodoros I Roumeliotis,James C Wright,Danielle Carson,Meirav Pevsner-Fischer,R Christopher D Furniss,Gordon Dougan,Mally Dori-Bachash,Lu Yu,Abigail Clements,James W Collins,Eran Elinav,Gerald J Larrouy-Maumus,Jyoti S Choudhary,Gad Frankel

Nature communications 8:15663 PubMed28580927

2017

The ATP-binding cassette transporter A1 regulates phosphoantigen release and Vγ9Vδ2 T cell activation by dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Castella,Joanna Kopecka,Patrizia Sciancalepore,Giorgia Mandili,Myriam Foglietta,Nico Mitro,Donatella Caruso,Francesco Novelli,Chiara Riganti,Massimo Massaia
View all publications
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Product promise

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