AB223056

Anti-ABCG8 antibody

Name: Anti-ABCG8 antibody (ab223056) | Abcam
Brand: Abcam Limited
SKU: AB223056
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(8 Publications)

Rabbit Polyclonal ABCG8 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP-binding cassette sub-family G member 8 aa 150-450.

View Alternative Names

ATP-binding cassette sub-family G member 8, Sterolin-2, ABCG8

1 Images

Supplier Data

Western blot - Anti-ABCG8 antibody (AB223056)

All lanes:

Western blot - Anti-ABCG8 antibody (ab223056) at 1/2000 dilution

Lane 1:

Mouse heart lysate

Lane 2:

Mouse lung lysate

Lane 3:

Mouse kidney lysate

Lane 4:

Mouse stomach lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG at 1/50000 dilution

Predicted band size: 76 kDa

Observed band size: 76 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human ATP-binding cassette sub-family G member 8 aa 150-450. The exact immunogen used to generate this antibody is proprietary information.

Q9H221

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCG8 also known as ATP-binding cassette sub-family G member 8 is a transporter protein involved in cholesterol homeostasis. It is a part of the ATP-binding cassette (ABC) family with an approximate mass of 74 kDa. ABCG8 is expressed in the liver and small intestine where it plays a role in the export of sterols. This protein functions as a half-transporter and pairs with ABCG5 to form a functional heterodimer which is essential for its function in cellular settings.

Biological function summary

ATP-binding cassette members like ABCG8 participate directly in the movement of lipids across cellular membranes. This transporter is part of a heterodimer complex with ABCG5 that mediates the excretion of sterols into the bile and intestinal lumen. The ABCG5/ABCG8 complex ensures the removal of excess cholesterol and plant sterols from the body maintaining lipid balance and preventing sterol accumulation.

Pathways

The cholesterol transport functions of ABCG8 integrate into the hepatic and intestinal cholesterol efflux pathways. It interacts with proteins and processes in the reverse cholesterol transport pathway and impacts the rate of cholesterol clearance. The ABCG5/ABCG8 heterodimer works alongside proteins like LDL receptors playing a part in a broader network ensuring cholesterol homeostasis and absorption is maintained at optimal levels.

Mutations or malfunctions in ABCG8 link to conditions such as sitosterolemia and gallstone disease. Sitosterolemia is characterized by the accumulation of plant sterols due to impaired function of the transporter complex which affects ABCG5 similarly since they function together. Additionally altered activity of ABCG8 can influence cholesterol crystal formation leading to gallstone development by disrupting the normal biliary sterol composition.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile (PubMed : 11099417, PubMed : 11452359, PubMed : 15054092, PubMed : 27144356). Required for normal sterol homeostasis (PubMed : 11099417, PubMed : 11452359, PubMed : 15054092). The heterodimer with ABCG5 has ATPase activity (PubMed : 16893193, PubMed : 20210363, PubMed : 27144356).

See full target information ATP-binding cassette sub-family G member 8

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:5015-5033 PubMed40860180

2025

Citrus Pectin Supplementation Alleviated Hepatic Lipid Accumulation through Gut Microbiota Indole Lactic Acid Promoting Hepatic Bile Acid Synthesis and Excretion.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijun Pan,Xinyuan Jin,Qing Li,Yuqing Zhou,Yupeng Zeng,Xin Wang,Yufeng Jin,Yu Chen,Dan Li,Wenhua Ling

Open life sciences 20:20221009 PubMed40756571

2025

Polydatin prevents cholesterol gallstone formation by regulating cholesterol metabolism via PPAR-γ signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Li,Xiaopeng Yu,Zhongxiao Zhou

Parasitology 152:381-394 PubMed40134311

2025

Characterization and microRNA quantification of plasma-derived extracellular vesicles in patients with infection.

Applications

Unspecified application

Species

Unspecified reactive species

Thunchanok Khammanee,Natakorn Nokchan,Piyatida Molika,Hansuk Buncherd,Kanitta Srinoun,Natta Tansila,Charinrat Saechan,Rachanida Praparatana,Nongyao Sawangjaroen,Suwannee Jitueakul,Chatree Ratcha,Churat Weeraphan,Jisnuson Svasti,Raphatphorn Navakanitworakul,Supinya Thanapongpichat

International journal of molecular medicine 55: PubMed40116083

2025

ABCG8‑mediated sterol efflux increases cancer cell progression via the LRP6/Wnt/β‑catenin signaling pathway in radiotherapy‑resistant MDA‑MB‑231 triple‑negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Young Shin Ko,Ju Yeong Won,Hana Jin,Nam Binh Nguyen,Yaeram Won,Vedaste Nsanzimana,Seung Pil Yun,Sang Won Park,Hye Jung Kim

Acta pharmaceutica Sinica. B 14:5267-5285 PubMed39807334

2025

Intestinal epithelial cell NCoR deficiency ameliorates obesity and metabolic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Shaocong Hou,Hengcai Yu,Caihong Liu,Andrew M F Johnson,Xingfeng Liu,Qian Jiang,Qijin Zhao,Lijuan Kong,Yanjun Wan,Xiaowei Xing,Yibing Chen,Jingwen Chen,Qing Wu,Peng Zhang,Changtao Jiang,Bing Cui,Pingping Li

Science advances 9:eadf1130 PubMed37540756

2023

Adseverin, an actin-binding protein, modulates hypertrophic chondrocyte differentiation and osteoarthritis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Byron Chan,Michael Glogauer,Yongqiang Wang,Jeffrey Wrana,Kin Chan,Frank Beier,Supinder Bali,Boris Hinz,Justin Parreno,Sajjad Ashraf,Rita Kandel

Molecular therapy. Nucleic acids 24:711-727 PubMed33996254

2021

HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARα axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Zhang,Huiqing Wen,Bangjian Peng,Jun Weng,Fanhong Zeng

Nan fang yi ke da xue xue bao = Journal of Southern Medical University 38:1020-1024 PubMed30187881

2018

[Lipid-lowering effect of propolis in mice with Triton-WR1339-induced hyperlipidemia and its mechanism for regulating lipid metabolism].

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqi Huang,Xiaoli Wu,Sishan Yan,Tian Lan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ABCG8 antibody

Western blot - Anti-ABCG8 antibody (AB223056)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise