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AB68949

Anti-ABHD12 antibody

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(2 Publications)

Mouse Polyclonal ABHD12 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ABHD12.

View Alternative Names

C20orf22, ABHD12, Lysophosphatidylserine lipase ABHD12, 2-arachidonoylglycerol hydrolase ABHD12, Abhydrolase domain-containing protein 12, Monoacylglycerol lipase ABHD12, Oxidized phosphatidylserine lipase ABHD12, hABHD12

1 Images
Western blot - Anti-ABHD12 antibody (AB68949)
  • WB

Unknown

Western blot - Anti-ABHD12 antibody (AB68949)

All lanes:

Western blot - Anti-ABHD12 antibody (ab68949) at 1/500 dilution

Lane 1:

ABHD12 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP conjugated at 1/2500 dilution

Predicted band size: 45 kDa

Observed band size: 45 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ABHD12.

Q8N2K0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABHD12 also known as alpha/beta-hydrolase domain-containing protein 12 is an enzyme that mechanically functions as a lipase. It plays a role in catalytic activity by hydrolyzing lysophospholipids and other lipid substrates. ABHD12 is a membrane-bound protein with a molecular mass of approximately 45 kDa. It is widely expressed in the human body with higher expressions noted in the brain immune system and liver.
Biological function summary

ABHD12 plays an important role in the immune and central nervous systems. It is not currently identified as part of a complex but is significant in regulating lipid signaling and homeostasis. By maintaining proper lysophosphatidylserine and lysophosphatidic acid levels ABHD12 influences neuroinflammation and neurodegenerative processes.

Pathways

ABHD12 interacts with lipid signaling and metabolic pathways. It is involved in the endocannabinoid signaling pathway and the lysophospholipid signaling pathway. Within these pathways important connections exist between ABHD12 and other proteins like MAGL (monoacylglycerol lipase) and FAAH (fatty acid amide hydrolase) which help modulate the levels of lipid messengers.

ABHD12 has connections to neurodegenerative diseases including PHARC syndrome and multiple sclerosis. Mutations in the ABHD12 gene result in PHARC syndrome which involves demyelination and peripheral neuropathy. In the context of multiple sclerosis ABHD12’s role in neuroinflammation relates it to proteins such as TNF-alpha and IL-1 beta due to their involvement in immune responses and inflammatory processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lysophosphatidylserine (LPS) lipase that mediates the hydrolysis of lysophosphatidylserine, a class of signaling lipids that regulates immunological and neurological processes (PubMed : 25290914, PubMed : 30237167, PubMed : 30420694, PubMed : 30643283, PubMed : 30720278). Represents a major lysophosphatidylserine lipase in the brain, thereby playing a key role in the central nervous system (By similarity). Also able to hydrolyze oxidized phosphatidylserine; oxidized phosphatidylserine is produced in response to severe inflammatory stress and constitutes a proapoptotic 'eat me' signal (PubMed : 30643283). Also has monoacylglycerol (MAG) lipase activity : hydrolyzes 2-arachidonoylglycerol (2-AG), thereby acting as a regulator of endocannabinoid signaling pathways (PubMed : 22969151, PubMed : 24027063). Has a strong preference for very-long-chain lipid substrates; substrate specificity is likely due to improved catalysis and not improved substrate binding (PubMed : 30237167).
See full target information ABHD12
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 293:16953-16963 PubMed30237167

2018

Biochemical characterization of the PHARC-associated serine hydrolase ABHD12 reveals its preference for very-long-chain lipids.

Applications

Unspecified application

Species

Unspecified reactive species

Alaumy Joshi,Minhaj Shaikh,Shubham Singh,Abinaya Rajendran,Amol Mhetre,Siddhesh S Kamat

Journal of lipid research 53:2413-24 PubMed22969151

2012

Biochemical and pharmacological characterization of human α/β-hydrolase domain containing 6 (ABHD6) and 12 (ABHD12).

Applications

WB

Species

Unspecified reactive species

Dina Navia-Paldanius,Juha R Savinainen,Jarmo T Laitinen
View all publications
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