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AB276068

Anti-ABHD14B antibody [011] - BSA and Azide free

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Rabbit Recombinant Monoclonal ABHD14B antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ABHD14B aa 1 to C-terminus.

View Alternative Names

CIB, ABHD14B, Putative protein-lysine deacylase ABHD14B, Alpha/beta hydrolase domain-containing protein 14B, CCG1-interacting factor B, Abhydrolase domain-containing protein 14B

2 Images
Immunoprecipitation - Anti-ABHD14B antibody [011] - BSA and Azide free (AB276068)
  • IP

Unknown

Immunoprecipitation - Anti-ABHD14B antibody [011] - BSA and Azide free (AB276068)

ABHD14B was immunoprecipitated from 0.5mg Jurkat (human T cell leukemia cell line from peripheral blood) and HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (Lane A and B, respectively) with 0.5 μL ab276068 and 15 μl of 50 % Protein G agarose. Western blot was perfoermed from immunoprecipitate using ab276068 at 1/500 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution.

Developed using the odssey technique.

Performed under reducing conditions.

All lanes:

Immunoprecipitation - Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068)

Predicted band size: 22 kDa

false

Western blot - Anti-ABHD14B antibody [011] - BSA and Azide free (AB276068)
  • WB

Unknown

Western blot - Anti-ABHD14B antibody [011] - BSA and Azide free (AB276068)

All lanes:

Western blot - Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068) at 1/500 dilution

All lanes:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

Predicted band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

011

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human ABHD14B aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96IU4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>0.2-1 μL/mg of lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein ABHD14B also known as abhydrolase domain containing 14B has a significant role in biochemical processes. It is about 42 kDa in mass. The ABHD14B protein functions as a serine hydrolase which means it belongs to a broad category of enzymes that hydrolyze ester bonds. It is expressed mostly in the liver and brain indicating its roles in various cellular functions in these tissues.
Biological function summary

ABHD14B plays a part in cellular metabolism and signal transduction. It interacts with other proteins within a multi-protein complex to regulate lipid homeostasis influencing the production and breakdown of lipid molecules. ABHD14B enzymes modulate these processes which are essential for maintaining cell membrane integrity and signaling events that control cell growth and differentiation.

Pathways

The involvement of ABHD14B includes the lipid signaling and metabolic regulation pathways. It has connections with the fatty acid metabolism pathway coordinating with proteins like ACC and FASN to manage fatty acid synthesis and degradation. Its activities impact lipid synthesis and degradation showing its importance in maintaining cellular energy balance and signaling.

Improper function of ABHD14B links to metabolic diseases such as hepatic steatosis also known as fatty liver disease. It also holds connections to neurological disorders owing to its expression in the brain. Proteins involved in these conditions like ACC in metabolic disorders or Tau in neurological diseases interact with ABHD14B indicating its potential role in these pathologies. Understanding these interactions may provide insights into therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as an atypical protein-lysine deacetylase in vitro (PubMed : 31478652). Catalyzes the deacetylation of lysine residues using CoA as substrate, generating acetyl-CoA and the free amine of protein-lysine residues (PubMed : 31478652). Additional experiments are however required to confirm the protein-lysine deacetylase activity in vivo (Probable). Has hydrolase activity towards various surrogate p-nitrophenyl (pNp) substrates, such as pNp-butyrate, pNp-acetate and pNp-octanoate in vitro, with a strong preference for pNp-acetate (PubMed : 14672934, PubMed : 31478652). May activate transcription (PubMed : 14672934).
See full target information ABHD14B
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Product promise

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