Rabbit Recombinant Monoclonal ABHD14B antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ABHD14B aa 1 to C-terminus.
Images
![Immunoprecipitation - Anti-ABHD14B antibody [011] - BSA and Azide free (AB276068), expandable thumbnail](https://content.abcam.com/products/images/abhd14b-antibody-011-bsa-and-azide-free-ab276068--immunoprecipitation-img35799.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ABHD14B antibody [011] - BSA and Azide free (AB276068)")
![Western blot - Anti-ABHD14B antibody [011] - BSA and Azide free (AB276068), expandable thumbnail](https://content.abcam.com/products/images/abhd14b-antibody-011-bsa-and-azide-free-ab276068--western-blot-img101171.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ABHD14B antibody [011] - BSA and Azide free (AB276068)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human ABHD14B aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96IU4
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 0.2-1 μL/mg of lysate |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Target data
Function
Acts as an atypical protein-lysine deacetylase in vitro (PubMed:31478652). Catalyzes the deacetylation of lysine residues using CoA as substrate, generating acetyl-CoA and the free amine of protein-lysine residues (PubMed:31478652). Additional experiments are however required to confirm the protein-lysine deacetylase activity in vivo (Probable). Has hydrolase activity towards various surrogate p-nitrophenyl (pNp) substrates, such as pNp-butyrate, pNp-acetate and pNp-octanoate in vitro, with a strong preference for pNp-acetate (PubMed:14672934, PubMed:31478652). May activate transcription (PubMed:14672934).
Alternative names
CIB, ABHD14B, Putative protein-lysine deacylase ABHD14B, Alpha/beta hydrolase domain-containing protein 14B, CCG1-interacting factor B, Abhydrolase domain-containing protein 14B
Recommended products
Rabbit Recombinant Monoclonal ABHD14B antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ABHD14B aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human ABHD14B aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96IU4
- Carrier free
- Yes
- Clone number
- 011
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein ABHD14B also known as abhydrolase domain containing 14B has a significant role in biochemical processes. It is about 42 kDa in mass. The ABHD14B protein functions as a serine hydrolase which means it belongs to a broad category of enzymes that hydrolyze ester bonds. It is expressed mostly in the liver and brain indicating its roles in various cellular functions in these tissues.
- Biological function summary
ABHD14B plays a part in cellular metabolism and signal transduction. It interacts with other proteins within a multi-protein complex to regulate lipid homeostasis influencing the production and breakdown of lipid molecules. ABHD14B enzymes modulate these processes which are essential for maintaining cell membrane integrity and signaling events that control cell growth and differentiation.
- Pathways
The involvement of ABHD14B includes the lipid signaling and metabolic regulation pathways. It has connections with the fatty acid metabolism pathway coordinating with proteins like ACC and FASN to manage fatty acid synthesis and degradation. Its activities impact lipid synthesis and degradation showing its importance in maintaining cellular energy balance and signaling.
- Associated diseases and disorders
Improper function of ABHD14B links to metabolic diseases such as hepatic steatosis also known as fatty liver disease. It also holds connections to neurological disorders owing to its expression in the brain. Proteins involved in these conditions like ACC in metabolic disorders or Tau in neurological diseases interact with ABHD14B indicating its potential role in these pathologies. Understanding these interactions may provide insights into therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Immunoprecipitation - Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068), expandable thumbnail](https://content.abcam.com/products/images/abhd14b-antibody-011-bsa-and-azide-free-ab276068--immunoprecipitation-img35799.jpg "Immunoprecipitation - Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068)")
Immunoprecipitation - Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068)
ABHD14B was immunoprecipitated from 0.5mg Jurkat (human T cell leukemia cell line from peripheral blood) and HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (Lane A and B, respectively) with 0.5 μL ab276068 and 15 μl of 50 % Protein G agarose. Western blot was perfoermed from immunoprecipitate using ab276068 at 1/500 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution.
Developed using the odssey technique.
Performed under reducing conditions.
All lanes: Immunoprecipitation - Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068)
Predicted band size: 22 kDa
![Western blot - Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068), expandable thumbnail](https://content.abcam.com/products/images/abhd14b-antibody-011-bsa-and-azide-free-ab276068--western-blot-img101171.jpg "Western blot - Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068)")
Western blot - Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068)
All lanes: Western blot - Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068) at 1/500 dilution
All lanes: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Predicted band size: 22 kDa
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