Rabbit Polyclonal ABHD14B antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ABHD14B.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human ABHD14B. Database link Q96IU4
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
| Cow | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 - 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
Target data
Function
Acts as an atypical protein-lysine deacetylase in vitro (PubMed:31478652). Catalyzes the deacetylation of lysine residues using CoA as substrate, generating acetyl-CoA and the free amine of protein-lysine residues (PubMed:31478652). Additional experiments are however required to confirm the protein-lysine deacetylase activity in vivo (Probable). Has hydrolase activity towards various surrogate p-nitrophenyl (pNp) substrates, such as pNp-butyrate, pNp-acetate and pNp-octanoate in vitro, with a strong preference for pNp-acetate (PubMed:14672934, PubMed:31478652). May activate transcription (PubMed:14672934).
Alternative names
CIB, ABHD14B, Putative protein-lysine deacylase ABHD14B, Alpha/beta hydrolase domain-containing protein 14B, CCG1-interacting factor B, Abhydrolase domain-containing protein 14B
Recommended products
Rabbit Polyclonal ABHD14B antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ABHD14B.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human ABHD14B. Database link Q96IU4
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein ABHD14B also known as abhydrolase domain containing 14B has a significant role in biochemical processes. It is about 42 kDa in mass. The ABHD14B protein functions as a serine hydrolase which means it belongs to a broad category of enzymes that hydrolyze ester bonds. It is expressed mostly in the liver and brain indicating its roles in various cellular functions in these tissues.
- Biological function summary
ABHD14B plays a part in cellular metabolism and signal transduction. It interacts with other proteins within a multi-protein complex to regulate lipid homeostasis influencing the production and breakdown of lipid molecules. ABHD14B enzymes modulate these processes which are essential for maintaining cell membrane integrity and signaling events that control cell growth and differentiation.
- Pathways
The involvement of ABHD14B includes the lipid signaling and metabolic regulation pathways. It has connections with the fatty acid metabolism pathway coordinating with proteins like ACC and FASN to manage fatty acid synthesis and degradation. Its activities impact lipid synthesis and degradation showing its importance in maintaining cellular energy balance and signaling.
- Associated diseases and disorders
Improper function of ABHD14B links to metabolic diseases such as hepatic steatosis also known as fatty liver disease. It also holds connections to neurological disorders owing to its expression in the brain. Proteins involved in these conditions like ACC in metabolic disorders or Tau in neurological diseases interact with ABHD14B indicating its potential role in these pathologies. Understanding these interactions may provide insights into therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-ABHD14B antibody (ab235436)
All lanes: Western blot - Anti-ABHD14B antibody (ab235436) at 1/500 dilution
Lane 1: Mouse small intestine lysate
Lane 2: Mouse spleen lysate
Lane 3: Mouse liver lysate
Lane 4: Mouse lung lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 22 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABHD14B antibody (ab235436)
Paraffin-embedded human liver tissue stained for ABHD14B using ab235436 at 1/100 dilution in immunohistochemical analysis.
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