AB111984

Anti-Abhd5/CGI-58 antibody

Name: Anti-Abhd5/CGI-58 antibody (ab111984) | Abcam
Brand: Abcam Limited
SKU: AB111984
Price: 595 USD
Availability: InStock

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(2 Publications)

Goat Polyclonal Abhd5/CGI-58 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ABHD5 aa 150-200.

View Alternative Names

NCIE2, CGI-58, ABHD5, 1-acylglycerol-3-phosphate O-acyltransferase ABHD5, Abhydrolase domain-containing protein 5, Lipid droplet-binding protein CGI-58

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody (AB111984)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody (AB111984)

ab111984, at 3.75 μg/ml, staining Abhd5/CGI-58 in formalin-fixed, paraffin-embedded Human pancreas tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody (AB111984)

ab111984, at 3.75 μg/ml, staining Abhd5/CGI-58 in formalin-fixed, paraffin-embedded Human kidney tissue by Immunohistochemistry.

Unknown

Western blot - Anti-Abhd5/CGI-58 antibody (AB111984)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-Abhd5/CGI-58 antibody (ab111984) at 0.2 µg/mL

All lanes:

NIH3T3 lysate (in RIPA buffer) at 35 µg

Predicted band size: 39 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ABHD5 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

Q8WTS1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.75 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-0.6 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Abhd5 also known as CGI-58 is a protein with significant importance in lipid metabolism. It has a molecular weight of approximately 39 kDa and is ubiquitously expressed in tissues throughout the body including adipose tissue and liver. This protein associates with lipid droplets within cells serving a role important for lipid handling and signaling. Abhd5 stands for the alpha/beta hydrolase domain-containing protein 5 indicative of its structure and enzymatic functions.

Biological function summary

The Abhd5/CGI-58 protein acts as a coactivator of adipose triglyceride lipase (ATGL) enhancing its role in triglyceride hydrolysis. This support is vital for energy release from triglycerides in fat cells. Abhd5 does not function alone; it exists within a protein complex where it modulates ATGL's catalytic activity. Furthermore Abhd5 is implicated in broader lipid signaling and metabolic control influencing various cellular responses through its roles within these complexes.

Pathways

Abhd5/CGI-58 is central to the lipid metabolism pathway particularly in the breakdown of triglycerides. By activating ATGL Abhd5 influences the lipolytic pathway which is important for mobilizing stored fats for energy use. In addition to its role with ATGL another protein perilipin regulates this pathway by interacting with lipid droplet-associated proteins including Abhd5 to control access to lipids. The protein influences the signaling crosstalk within metabolic pathways coordinating metabolic responses within the cell.

The Abhd5/CGI-58 protein has ties to conditions like lipodystrophy and Chanarin-Dorfman syndrome. Deficiencies or mutations in the CGI-58 protein can impair lipid metabolism leading to abnormal fat distribution and storage. In these diseases the lack of Abhd5 activity affects the interactions with proteins like ATGL which is responsible for triglyceride breakdown. Research continues to explore the relationship between Abhd5 dysfunction and these metabolic disorders aiming to clarify its role in the etiology of such conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Coenzyme A-dependent lysophosphatidic acid acyltransferase that catalyzes the transfer of an acyl group on a lysophosphatidic acid (PubMed : 18606822). Functions preferentially with 1-oleoyl-lysophosphatidic acid followed by 1-palmitoyl-lysophosphatidic acid, 1-stearoyl-lysophosphatidic acid and 1-arachidonoyl-lysophosphatidic acid as lipid acceptor. Functions preferentially with arachidonoyl-CoA followed by oleoyl-CoA as acyl group donors (By similarity). Functions in phosphatidic acid biosynthesis (PubMed : 18606822). May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2 (PubMed : 16679289). Involved in keratinocyte differentiation (PubMed : 18832586). Regulates lipid droplet fusion (By similarity).

See full target information ABHD5

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Food science & nutrition 11:1223-1231 PubMed36911831

2023

4-PBA inhibits hypoxia-induced lipolysis in rat adipose tissue and lipid accumulation in the liver through regulating ER stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yanlei Xiong,Yueming Wang,Yanlian Xiong,Lianghong Teng

The Journal of biological chemistry 287:8444-56 PubMed22267746

2012

Angiopoietin-like 4 (Angptl4) protein is a physiological mediator of intracellular lipolysis in murine adipocytes.

Applications

Species

Mouse

Nora E Gray,Lily N Lam,Karen Yang,Anna Y Zhou,Suneil Koliwad,Jen-Chywan Wang

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Abhd5/CGI-58 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody (AB111984)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody (AB111984)

Western blot - Anti-Abhd5/CGI-58 antibody (AB111984)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise