AB59488

Anti-Abhd5/CGI-58 antibody

Name: Anti-Abhd5/CGI-58 antibody (ab59488) | Abcam
Brand: Abcam Limited
SKU: AB59488
Price: 525 USD
Availability: InStock

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(4 Publications )

Goat Polyclonal Abhd5/CGI-58 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ABHD5 aa 150-200.

2 Images

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Western blot - Anti-Abhd5/CGI-58 antibody (AB59488)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-Abhd5/CGI-58 antibody (ab59488) at 1 µg/mL

All lanes:

A431 cell line lysate (35µg protein in RIPA buffer)

Predicted band size: 39 kDa

Observed band size: 38 kDa

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Western blot - Anti-Abhd5/CGI-58 antibody (AB59488)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-Abhd5/CGI-58 antibody (ab59488) at 0.2 µg/mL

All lanes:

NIH-3T3 cell lysate (RIPA buffer) at 35 µg

Predicted band size: 39 kDa

Observed band size: 40 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human ABHD5 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

Q8WTS1

Specificity

This antibody is not expected to cross-react with ABHD4.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-0.6 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. Additional faint bands of 26kDa and 18kDa were observed in NIH-3T3. These bands were successfully blocked by incubation with the immunizing peptide.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-0.6 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. Additional faint bands of 26kDa and 18kDa were observed in NIH-3T3. These bands were successfully blocked by incubation with the immunizing peptide.</p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Abhd5 also known as CGI-58 is a protein with significant importance in lipid metabolism. It has a molecular weight of approximately 39 kDa and is ubiquitously expressed in tissues throughout the body including adipose tissue and liver. This protein associates with lipid droplets within cells serving a role important for lipid handling and signaling. Abhd5 stands for the alpha/beta hydrolase domain-containing protein 5 indicative of its structure and enzymatic functions.

Biological function summary

The Abhd5/CGI-58 protein acts as a coactivator of adipose triglyceride lipase (ATGL) enhancing its role in triglyceride hydrolysis. This support is vital for energy release from triglycerides in fat cells. Abhd5 does not function alone; it exists within a protein complex where it modulates ATGL's catalytic activity. Furthermore Abhd5 is implicated in broader lipid signaling and metabolic control influencing various cellular responses through its roles within these complexes.

Pathways

Abhd5/CGI-58 is central to the lipid metabolism pathway particularly in the breakdown of triglycerides. By activating ATGL Abhd5 influences the lipolytic pathway which is important for mobilizing stored fats for energy use. In addition to its role with ATGL another protein perilipin regulates this pathway by interacting with lipid droplet-associated proteins including Abhd5 to control access to lipids. The protein influences the signaling crosstalk within metabolic pathways coordinating metabolic responses within the cell.

The Abhd5/CGI-58 protein has ties to conditions like lipodystrophy and Chanarin-Dorfman syndrome. Deficiencies or mutations in the CGI-58 protein can impair lipid metabolism leading to abnormal fat distribution and storage. In these diseases the lack of Abhd5 activity affects the interactions with proteins like ATGL which is responsible for triglyceride breakdown. Research continues to explore the relationship between Abhd5 dysfunction and these metabolic disorders aiming to clarify its role in the etiology of such conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Coenzyme A-dependent lysophosphatidic acid acyltransferase that catalyzes the transfer of an acyl group on a lysophosphatidic acid (PubMed : 18606822). Functions preferentially with 1-oleoyl-lysophosphatidic acid followed by 1-palmitoyl-lysophosphatidic acid, 1-stearoyl-lysophosphatidic acid and 1-arachidonoyl-lysophosphatidic acid as lipid acceptor. Functions preferentially with arachidonoyl-CoA followed by oleoyl-CoA as acyl group donors (By similarity). Functions in phosphatidic acid biosynthesis (PubMed : 18606822). May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2 (PubMed : 16679289). Involved in keratinocyte differentiation (PubMed : 18832586). Regulates lipid droplet fusion (By similarity).

See full target information ABHD5

Alternative Names

NCIE2, CGI-58, ABHD5, 1-acylglycerol-3-phosphate O-acyltransferase ABHD5, Abhydrolase domain-containing protein 5, Lipid droplet-binding protein CGI-58

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:888 PubMed35042927

2022

Responsiveness of PNPLA3 and lipid-related transcription factors is dependent upon fatty acid profile in primary bovine hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Sophia J Erb,Tawny L Chandler,Heather M White

Nature metabolism 1:1157-1167 PubMed31742248

2019

The lipid droplet-associated protein ABHD5 protects the heart through proteolysis of HDAC4.

Applications

Unspecified application

Species

Unspecified reactive species

Zegeye H Jebessa,Kumar D Shanmukha,Matthias Dewenter,Lorenz H Lehmann,Chang Xu,Friederike Schreiter,Dominik Siede,Xue-Min Gong,Barbara C Worst,Giuseppina Federico,Sven W Sauer,Tamas Fischer,Lisa Wechselberger,Oliver J Müller,Samuel Sossalla,Christoph Dieterich,Patrick Most,Herrmann-Josef Gröne,Cedric Moro,Monika Oberer,Guenter Haemmerle,Hugo A Katus,Jens Tyedmers,Johannes Backs

Frontiers in pharmacology 10:1154 PubMed31680950

2019

Ginsenoside Rb1 Induces Beta 3 Adrenergic Receptor-Dependent Lipolysis and Thermogenesis in 3T3-L1 Adipocytes and Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Seona Lim,Jinbong Park,Jae-Young Um

Molecular and cellular biology 34:2339-49 PubMed24732799

2014

KSRP and MicroRNA 145 are negative regulators of lipolysis in white adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Yu Lin,Chu-Fang Chou,Matteo Giovarelli,Paola Briata,Roberto Gherzi,Ching-Yi Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Abhd5/CGI-58 antibody

Western blot - Anti-Abhd5/CGI-58 antibody (AB59488)

Western blot - Anti-Abhd5/CGI-58 antibody (AB59488)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (4)

Product promise