AB73551

Anti-Abhd5/CGI-58 antibody

Name: Anti-Abhd5/CGI-58 antibody (ab73551) | Abcam
Brand: Abcam Limited
SKU: AB73551
Price: 505 USD
Availability: InStock

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Polyclonal Abhd5/CGI-58 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

NCIE2, CGI-58, ABHD5, 1-acylglycerol-3-phosphate O-acyltransferase ABHD5, Abhydrolase domain-containing protein 5, Lipid droplet-binding protein CGI-58

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Abhd5/CGI-58 antibody (AB73551)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Abhd5/CGI-58 antibody (AB73551)

ICC/IF image of ab73551 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73551, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Unknown

Western blot - Anti-Abhd5/CGI-58 antibody (AB73551)

All lanes:

Western blot - Anti-Abhd5/CGI-58 antibody (ab73551) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg

Lane 4:

Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 39 kDa

Observed band size: 42 kDa

true

Exposure time: 4min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Abhd5 also known as CGI-58 is a protein with significant importance in lipid metabolism. It has a molecular weight of approximately 39 kDa and is ubiquitously expressed in tissues throughout the body including adipose tissue and liver. This protein associates with lipid droplets within cells serving a role important for lipid handling and signaling. Abhd5 stands for the alpha/beta hydrolase domain-containing protein 5 indicative of its structure and enzymatic functions.

Biological function summary

The Abhd5/CGI-58 protein acts as a coactivator of adipose triglyceride lipase (ATGL) enhancing its role in triglyceride hydrolysis. This support is vital for energy release from triglycerides in fat cells. Abhd5 does not function alone; it exists within a protein complex where it modulates ATGL's catalytic activity. Furthermore Abhd5 is implicated in broader lipid signaling and metabolic control influencing various cellular responses through its roles within these complexes.

Pathways

Abhd5/CGI-58 is central to the lipid metabolism pathway particularly in the breakdown of triglycerides. By activating ATGL Abhd5 influences the lipolytic pathway which is important for mobilizing stored fats for energy use. In addition to its role with ATGL another protein perilipin regulates this pathway by interacting with lipid droplet-associated proteins including Abhd5 to control access to lipids. The protein influences the signaling crosstalk within metabolic pathways coordinating metabolic responses within the cell.

The Abhd5/CGI-58 protein has ties to conditions like lipodystrophy and Chanarin-Dorfman syndrome. Deficiencies or mutations in the CGI-58 protein can impair lipid metabolism leading to abnormal fat distribution and storage. In these diseases the lack of Abhd5 activity affects the interactions with proteins like ATGL which is responsible for triglyceride breakdown. Research continues to explore the relationship between Abhd5 dysfunction and these metabolic disorders aiming to clarify its role in the etiology of such conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Coenzyme A-dependent lysophosphatidic acid acyltransferase that catalyzes the transfer of an acyl group on a lysophosphatidic acid (PubMed : 18606822). Functions preferentially with 1-oleoyl-lysophosphatidic acid followed by 1-palmitoyl-lysophosphatidic acid, 1-stearoyl-lysophosphatidic acid and 1-arachidonoyl-lysophosphatidic acid as lipid acceptor. Functions preferentially with arachidonoyl-CoA followed by oleoyl-CoA as acyl group donors (By similarity). Functions in phosphatidic acid biosynthesis (PubMed : 18606822). May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2 (PubMed : 16679289). Involved in keratinocyte differentiation (PubMed : 18832586). Regulates lipid droplet fusion (By similarity).

See full target information ABHD5

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 27:1528-1540.e7 PubMed31042478

2019

Identification of Metabolically Distinct Adipocyte Progenitor Cells in Human Adipose Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Arthe Raajendiran,Geraldine Ooi,Jackie Bayliss,Paul E O'Brien,Ralf B Schittenhelm,Ashlee K Clark,Renea A Taylor,Matthew S Rodeheffer,Paul R Burton,Matthew J Watt

Journal of cell science 132: PubMed30559250

2019

Perilipin-2 is critical for efficient lipoprotein and hepatitis C virus particle production.

Applications

Unspecified application

Species

Unspecified reactive species

Susan Lassen,Cordula Grüttner,Van Nguyen-Dinh,Eva Herker

PloS one 9:e103062 PubMed25054327

2014

Cellular localization and associations of the major lipolytic proteins in human skeletal muscle at rest and during exercise.

Applications

IHC-Fr

Species

Human

Rachael R Mason,Ruth C R Meex,Aaron P Russell,Benedict J Canny,Matthew J Watt

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Abhd5/CGI-58 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Abhd5/CGI-58 antibody (AB73551)

Western blot - Anti-Abhd5/CGI-58 antibody (AB73551)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise