Rabbit Recombinant Monoclonal Abi-1 phospho Y435 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] - BSA and Azide free (AB247847), expandable thumbnail](https://content.abcam.com/products/images/abi-1-phospho-y435-antibody-ep3954-bsa-and-azide-free-ab247847--western-blot-img154428.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] - BSA and Azide free (AB247847)")
![Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] - BSA and Azide free (AB247847), expandable thumbnail](https://content.abcam.com/products/images/abi-1-phospho-y435-antibody-ep3954-bsa-and-azide-free-ab247847--western-blot-img430004.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] - BSA and Azide free (AB247847)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons.
Alternative names
SSH3BP1, ABI1, Abl interactor 1, Abelson interactor 1, Abl-binding protein 4, Eps8 SH3 domain-binding protein, Nap1-binding protein, Spectrin SH3 domain-binding protein 1, e3B1, Abi-1, AblBP4, Eps8-binding protein, Nap1BP
Recommended products
Rabbit Recombinant Monoclonal Abi-1 phospho Y435 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP3954
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247847 is the carrier-free version of Anti-Abi-1 (phospho Y435) antibody [EP3954] ab109395.
The production method for this product has been changed from hybridoma to recombniant on 16th April 2024
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Abi-1 protein also known as ABI family member 1 is a small adapter protein with a molecular mass of about 55 kDa. It is mainly expressed in brain and hematopoietic tissues. Abi-1 contains several domains allowing it to interact with a variety of signaling and cytoskeletal proteins. Its role as a linker connects various cellular components and facilitates communication between them important for the dynamic regulation of the actin cytoskeleton.
- Biological function summary
Abi-1 influences actin polymerization and cell motility by associating with the WAVE complex. This complex plays an important function in mediating signal transduction from small GTPases to the actin cytoskeleton. Abi-1 helps to organize the cytoskeleton to enable cell movements and morphological changes. Moreover Abi-1 is involved in the regulation of signaling pathways linked with cell growth and differentiation. These functions position Abi-1 as an important factor in maintaining normal cellular function.
- Pathways
Abi-1 operates inside the WAVE regulatory complex which affects the Rac1 signaling pathway. This pathway is essential for actin polymerization and cellular migration and involves proteins like N-WASP and Arp2/3 complex. Through its interaction with Rac1 and associated proteins Abi-1 greatly affects cytoskeletal reorganization demonstrating its importance in normal cellular movement and immune cell function.
- Associated diseases and disorders
Alterations in Abi-1 have associations with cancer particularly in leukemia. Abi-1 plays a role in oncogenic processes by interacting with proteins like BCR-ABL which is implicated in the pathogenesis of chronic myeloid leukemia. Additionally Abi-1 can contribute to neurological disorders due to its expression in brain tissues but the mechanisms remain less clearly understood. The disorder-associated signaling dynamics make Abi-1 a promising research focus for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] - BSA and Azide free (ab247847), expandable thumbnail](https://content.abcam.com/products/images/abi-1-phospho-y435-antibody-ep3954-bsa-and-azide-free-ab247847--western-blot-img154428.jpg "Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] - BSA and Azide free (ab247847)")
Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] - BSA and Azide free (ab247847)
This data was developed using Anti-Abi-1 (phospho Y435) antibody [EP3954] ab109395, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] - BSA and Azide free (ab247847) at 1/10000 dilution
All lanes: Jurkat cell lysate, untreated at 10 µg
Predicted band size: 55 kDa
![Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] - BSA and Azide free (ab247847), expandable thumbnail](https://content.abcam.com/products/images/abi-1-phospho-y435-antibody-ep3954-bsa-and-azide-free-ab247847--western-blot-img430004.jpg "Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] - BSA and Azide free (ab247847)")
Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] - BSA and Azide free (ab247847)
This data was developed using Anti-Abi-1 (phospho Y435) antibody [EP3954] ab109395, the same antibody clone in a different buffer formulation.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used a GAPDH loading control
All lanes: Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] (Anti-Abi-1 (phospho Y435) antibody [EP3954] ab109395) at 1/10000 dilution
Lane 1: Untreated Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 15 µg with 5% NFDM/TBST
Lane 2: Raji treated with 1mM pervanadate for 30mins whole cell lysate at 15 µg with 5% NFDM/TBST
Lane 3: Raji treated with 1mM pervanadate for 30mins whole cell lysate, then the membrane was incubated with phosphatase at 15 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Exposure time: 7s
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