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AB108340

Anti-ABI2 antibody [EPR3906]

5

(2 Reviews)

|

(2 Publications)

Rabbit Recombinant Monoclonal ABI2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

ARGBPIA, ABI2, Abl interactor 2, Abelson interactor 2, Abl-binding protein 3, Arg-binding protein 1, Abi-2, AblBP3, ArgBP1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABI2 antibody [EPR3906] (AB108340)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABI2 antibody [EPR3906] (AB108340)

ab108340 at 1/250 staining ABI2 in paraffin-embedded Human brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-ABI2 antibody [EPR3906] (AB108340)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ABI2 antibody [EPR3906] (AB108340)

ab108340, at 1/500, staining ABI2 in HeLa cells by Immunofluorescence.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3906

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABI2 also known as Abl-Interactor 2 is a protein encoded by the ABI2 gene. It has a molecular mass of approximately 53 kDa. ABI2 functions as a regulator of actin cytoskeleton dynamics and participates in processes involving cell motility and morphology. This protein is mainly expressed in neuronal tissues and is found in the cytoplasm suggesting its involvement in intracellular signaling pathways where actin rearrangement is important.
Biological function summary

ABI2 impacts cellular functions by interacting with the WAVE (WASP-family verprolin-homologous) complex. This interaction facilitates the regulation of actin polymerization important for processes such as cell migration and synaptic plasticity. ABI2 through the WAVE complex plays a role in the formation of lamellipodia cellular protrusions important for movement. This regulation ensures that cells respond effectively to external stimuli by reorganizing their cytoskeleton.

Pathways

The protein contributes significantly to the regulation of the Rac1 signaling pathway important for cytoskeletal remodeling. ABI2 works closely with proteins such as NCK proteins and phosphoinositides that are part of this pathway. Furthermore ABI2 interacts with the PAK family of kinases influencing downstream signaling that controls cell cycle progression and survival. ABI2’s role in these pathways highlights its importance in maintaining cellular architecture and dynamics.

ABI2 is implicated in neurological conditions particularly Alzheimer's disease and schizophrenia. Its interaction with other proteins like APP (amyloid precursor protein) suggests a role in synaptic dysfunction seen in these disorders. ABI2’s involvement in actin dynamics connects it to synaptic defects that may contribute to cognitive impairments in Alzheimer's disease. Understanding ABI2’s function and interactions offers insights into therapeutic targets for these neurological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulator of actin cytoskeleton dynamics underlying cell motility and adhesion. Functions as a component of the WAVE complex, which activates actin nucleating machinery Arp2/3 to drive lamellipodia formation (PubMed : 21107423). Acts as a regulator and substrate of nonreceptor tyrosine kinases ABL1 and ABL2 involved in processes linked to cell growth and differentiation. Positively regulates ABL1-mediated phosphorylation of ENAH, which is required for proper polymerization of nucleated actin filaments at the leading edge (PubMed : 10498863, PubMed : 7590236, PubMed : 8649853). Contributes to the regulation of actin assembly at the tips of neuron projections. In particular, controls dendritic spine morphogenesis and may promote dendritic spine specification toward large mushroom-type spines known as repositories of memory in the brain (By similarity). In hippocampal neurons, may mediate actin-dependent BDNF-NTRK2 early endocytic trafficking that triggers dendrite outgrowth (By similarity). Participates in ocular lens morphogenesis, likely by regulating lamellipodia-driven adherens junction formation at the epithelial cell-secondary lens fiber interface (By similarity). Also required for nascent adherens junction assembly in epithelial cells (PubMed : 15572692).
See full target information ABI2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 19:3384-3390 PubMed32266037

2020

miR-25 expression is upregulated in pancreatic ductal adenocarcinoma and promotes cell proliferation by targeting ABI2.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Lu,Ling Zhang,Shan Lu,Dujiang Yang,Jun Ye,Mao Li,Weiming Hu

Oncogenesis 3:e90 PubMed24590311

2014

Synergistic effects of proteasome inhibitor carfilzomib in combination with tyrosine kinase inhibitors in imatinib-sensitive and -resistant chronic myeloid leukemia models.

Applications

WB

Species

Human

L J Crawford,E T Chan,M Aujay,T L Holyoake,J V Melo,H G Jorgensen,S Suresh,B Walker,A E Irvine
View all publications
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Product promise

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