AB133593

Anti-ABI2 antibody [EPR7069]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal ABI2 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 1 publication.

View Alternative Names

ARGBPIA, ABI2, Abl interactor 2, Abelson interactor 2, Abl-binding protein 3, Arg-binding protein 1, Abi-2, AblBP3, ArgBP1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABI2 antibody [EPR7069] (AB133593)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABI2 antibody [EPR7069] (AB133593)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling ABI2 with ab133593 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABI2 antibody [EPR7069] (AB133593)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling ABI2 with ab133593 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ABI2 antibody [EPR7069] (AB133593)

All lanes:

Western blot - Anti-ABI2 antibody [EPR7069] (ab133593) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

PC-12 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 56 kDa

false

OI-RD Scanning - Anti-ABI2 antibody [EPR7069] (AB133593)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ABI2 antibody [EPR7069] (AB133593)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7069

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABI2 also known as Abl-Interactor 2 is a protein encoded by the ABI2 gene. It has a molecular mass of approximately 53 kDa. ABI2 functions as a regulator of actin cytoskeleton dynamics and participates in processes involving cell motility and morphology. This protein is mainly expressed in neuronal tissues and is found in the cytoplasm suggesting its involvement in intracellular signaling pathways where actin rearrangement is important.

Biological function summary

ABI2 impacts cellular functions by interacting with the WAVE (WASP-family verprolin-homologous) complex. This interaction facilitates the regulation of actin polymerization important for processes such as cell migration and synaptic plasticity. ABI2 through the WAVE complex plays a role in the formation of lamellipodia cellular protrusions important for movement. This regulation ensures that cells respond effectively to external stimuli by reorganizing their cytoskeleton.

Pathways

The protein contributes significantly to the regulation of the Rac1 signaling pathway important for cytoskeletal remodeling. ABI2 works closely with proteins such as NCK proteins and phosphoinositides that are part of this pathway. Furthermore ABI2 interacts with the PAK family of kinases influencing downstream signaling that controls cell cycle progression and survival. ABI2’s role in these pathways highlights its importance in maintaining cellular architecture and dynamics.

ABI2 is implicated in neurological conditions particularly Alzheimer's disease and schizophrenia. Its interaction with other proteins like APP (amyloid precursor protein) suggests a role in synaptic dysfunction seen in these disorders. ABI2’s involvement in actin dynamics connects it to synaptic defects that may contribute to cognitive impairments in Alzheimer's disease. Understanding ABI2’s function and interactions offers insights into therapeutic targets for these neurological conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Regulator of actin cytoskeleton dynamics underlying cell motility and adhesion. Functions as a component of the WAVE complex, which activates actin nucleating machinery Arp2/3 to drive lamellipodia formation (PubMed : 21107423). Acts as a regulator and substrate of nonreceptor tyrosine kinases ABL1 and ABL2 involved in processes linked to cell growth and differentiation. Positively regulates ABL1-mediated phosphorylation of ENAH, which is required for proper polymerization of nucleated actin filaments at the leading edge (PubMed : 10498863, PubMed : 7590236, PubMed : 8649853). Contributes to the regulation of actin assembly at the tips of neuron projections. In particular, controls dendritic spine morphogenesis and may promote dendritic spine specification toward large mushroom-type spines known as repositories of memory in the brain (By similarity). In hippocampal neurons, may mediate actin-dependent BDNF-NTRK2 early endocytic trafficking that triggers dendrite outgrowth (By similarity). Participates in ocular lens morphogenesis, likely by regulating lamellipodia-driven adherens junction formation at the epithelial cell-secondary lens fiber interface (By similarity). Also required for nascent adherens junction assembly in epithelial cells (PubMed : 15572692).

See full target information ABI2

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 66:60 PubMed39992672

2025

Untargeted Tear Proteomics in a Large South-Asian Cohort Reveals Inflammatory Signaling, ECM Remodeling, and Altered Metabolism in Keratoconus.

Applications

Unspecified application

Species

Unspecified reactive species

Ramaraj Kannan,Rohit Shetty,Trailokyanath Panigrahi,Siew Kwan Koh,Pooja Khamar,Vrushali Deshpande,Rudy M M A Nuijts,Marlies Gijs,Krishnatej Nishtala,Lei Zhou,Arkasubhra Ghosh

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ABI2 antibody [EPR7069]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABI2 antibody [EPR7069] (AB133593)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABI2 antibody [EPR7069] (AB133593)

Western blot - Anti-ABI2 antibody [EPR7069] (AB133593)

OI-RD Scanning - Anti-ABI2 antibody [EPR7069] (AB133593)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise