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Rabbit Recombinant Monoclonal ABI2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Rat samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWB
Human
Tested
Not recommended
Not recommended
Tested
Mouse
Predicted
Not recommended
Not recommended
Predicted
Rat
Expected
Not recommended
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Human, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Human, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human, Rat

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Target data

Function

Regulator of actin cytoskeleton dynamics underlying cell motility and adhesion. Functions as a component of the WAVE complex, which activates actin nucleating machinery Arp2/3 to drive lamellipodia formation (PubMed:21107423). Acts as regulator and substrate of nonreceptor tyrosine kinases ABL1 and ABL2 involved in processes linked to cell growth and differentiation. Positively regulates ABL1-mediated phosphorylation of ENAH, which is required for proper polymerization of nucleated actin filaments at the leading edge (PubMed:7590236, PubMed:8649853, PubMed:10498863). Contributes to the regulation of actin assembly at the tips of neuron projections. In particular, controls dendritic spine morphogenesis and may promote dendritic spine specification toward large mushroom-type spines known as repositories of memory in the brain (By similarity). In hippocampal neurons, may mediate actin-dependent BDNF-NTRK2 early endocytic trafficking that triggers dendrite outgrowth (By similarity). Participates in ocular lens morphogenesis, likely by regulating lamellipodia-driven adherens junction formation at the epithelial cell-secondary lens fiber interface (By similarity). Also required for nascent adherens junction assembly in epithelial cells (PubMed:15572692).

Alternative names

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Rabbit Recombinant Monoclonal ABI2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Rat samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Carrier free

Yes

Clone number

EPR7069

Purification technique

Affinity purification Protein A

Dissociation constant

9.2 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab248570 is the carrier-free version of ab133593.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-ABI2 antibody [EPR7069] - BSA and Azide free (ab248570), expandable thumbnail

    Western blot - Anti-ABI2 antibody [EPR7069] - BSA and Azide free (ab248570)

    This data was developed using ab133593, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-ABI2 antibody [EPR7069] (AB133593) at 1/1000 dilution

    Lane 1: K562 cell lysate at 10 µg

    Lane 2: PC-12 cell lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Lane 4: SH-SY5Y cell lysate at 10 µg

    Secondary

    All lanes: HRP labeled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 56 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABI2 antibody [EPR7069] - BSA and Azide free (ab248570), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABI2 antibody [EPR7069] - BSA and Azide free (ab248570)

    This data was developed using ab133593, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling ABI2 with ab133593 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABI2 antibody [EPR7069] - BSA and Azide free (ab248570), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABI2 antibody [EPR7069] - BSA and Azide free (ab248570)

    This data was developed using ab133593, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling ABI2 with ab133593 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-ABI2 antibody [EPR7069] - BSA and Azide free (ab248570), expandable thumbnail

    OI-RD Scanning - Anti-ABI2 antibody [EPR7069] - BSA and Azide free (ab248570)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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