AB134134

Anti-ABL1 + ABL2 antibody [EPR1222(2)]

RabMAb
Recombinant
What is this?

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(8 Publications)

Rabbit Recombinant Monoclonal ABL2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

View Alternative Names

ABLL, ARG, ABL2, Tyrosine-protein kinase ABL2, Abelson murine leukemia viral oncogene homolog 2, Abelson tyrosine-protein kinase 2, Abelson-related gene protein, Tyrosine-protein kinase ARG, ABL, JTK7, ABL1, Tyrosine-protein kinase ABL1, Abelson murine leukemia viral oncogene homolog 1, Abelson tyrosine-protein kinase 1, Proto-oncogene c-Abl, p150

3 Images

Flow Cytometry (Intracellular) - Anti-ABL1 + ABL2 antibody [EPR1222(2)] (AB134134)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-ABL1 + ABL2 antibody [EPR1222(2)] (AB134134)

Intracellular flow cytometric analysis of permeabilized Jurkat cells labelling ABL2 (red) using ab134134 at 1/10 dilution or using a Rabbit IgG negative control (green)

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Western blot - Anti-ABL1 + ABL2 antibody [EPR1222(2)] (AB134134)

All lanes:

Western blot - Anti-ABL1 + ABL2 antibody [EPR1222(2)] (ab134134) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 3:

K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 10 µg

Lane 4:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 10 µg

Lane 5:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 6:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 7:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti rabbit at 1/2000 dilution

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OI-RD Scanning - Anti-ABL1 + ABL2 antibody [EPR1222(2)] (AB134134)

OI-RD Scanning

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OI-RD Scanning - Anti-ABL1 + ABL2 antibody [EPR1222(2)] (AB134134)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1222(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABL1 and ABL2 also known as c-Abl and Arg are non-receptor tyrosine kinases that play significant roles in cellular processes. These proteins have molecular masses of approximately 120 and 140 kilodaltons respectively. ABL1 and ABL2 share structural similarities with other kinases but have unique regions that allow them to participate in specific cellular tasks. They are widely expressed in various tissues including hematopoietic cells indicating their involvement in diverse physiological functions.

Biological function summary

ABL1 and ABL2 contribute to essential cellular processes such as cell differentiation cell division adhesion and stress response. These kinases do not typically form complexes but they can interact with other proteins to fulfill their roles. They modulate cytoskeletal dynamics impacting cellular architecture and motility and are important in regulating responses to DNA damage. Their activity depends on phosphorylation events which can both activate and inhibit their functions.

Pathways

ABL1 and ABL2 participate in the regulation of the MAPK and p53 pathways. They influence cellular responses to growth factors and stress. In the MAPK pathway ABL1 and ABL2 interact with proteins like Grb2 and SOS mediating signal transduction that impacts cellular proliferation. Their roles in the p53 pathway involve modulating apoptosis and cell cycle arrest important for maintaining genome integrity.

ABL1 is strongly associated with chronic myeloid leukemia (CML) particularly through its fusion with the BCR gene to form the BCR-ABL oncogene. This oncogene results in unregulated tyrosine kinase activity that leads to excessive cellular proliferation. ABL2 has connections to solid tumors and neuropathies though its role is less defined than ABL1. Both kinases emerge as potential targets for therapeutic interventions in cancers and disorders related to dysregulated kinase activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 also acts as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1. Positively regulates chemokine-mediated T-cell migration, polarization, and homing to lymph nodes and immune-challenged tissues, potentially via activation of NEDD9/HEF1 and RAP1 (By similarity).

See full target information ABL2

Additional targets

ABL1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 27:1021-1034 PubMed40419795

2025

Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

James R W Conway,Omkar Joshi,Jasmin Kaivola,Gautier Follain,Michalis Gounis,David Kühl,Johanna Ivaska

Journal of Cancer 15:5719-5728 PubMed39308677

2024

High expression of ABL2 promotes gastric cancer cells migration, invasion and proliferation via the TGF-β and YAP signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Liu,Tao Jin,Ruiyun Chen,Renjie Miao,Yong Zhou,Shihe Shao

Proceedings of the National Academy of Sciences of the United States of America 120:e2219885120 PubMed37094151

2023

Consequences of poly(ethylene oxide) and poloxamer P188 on transcription in healthy and stressed myoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Adelyn A Crabtree,Natalie Boehnke,Frank S Bates,Benjamin J Hackel

Technology in cancer research & treatment 20:1533033821990009 PubMed33550941

2021

microRNA-1296 Inhibits Glioma Cell Growth by Targeting ABL2.

Applications

Unspecified application

Species

Unspecified reactive species

Gaolian Zhang,Meng Xia,Jianhui Guo,Yi Huang,Jianrong Huang,Kecong Wei,Xiaoning Zhang,Jing Zeng,Weibin Liang

Cellular and molecular neurobiology 41:431-448 PubMed32399753

2020

Abelson Kinases Mediate the Depression of Spontaneous Synaptic Activity Induced by Amyloid Beta 1-42 Peptides.

Applications

Unspecified application

Species

Unspecified reactive species

M Reichenstein,N Borovok,A Sheinin,T Brider,I Michaelevski

Journal of cellular biochemistry 115:1269-76 PubMed24464651

2014

miR-20a promotes prostate cancer invasion and migration through targeting ABL2.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Fei Qiang,Zheng-Wei Zhang,Qian Liu,Nan Sun,Liang-Liang Pan,Jing Shen,Tong Li,Chen Yun,Hui Li,Li-Hua Shi

Biochemical and biophysical research communications 440:197-203 PubMed23985350

2013

A humanin analog decreases oxidative stress and preserves mitochondrial integrity in cardiac myoblasts.

Applications

Species

Unspecified reactive species

Laura E Klein,Lingguang Cui,Zhenwei Gong,Kai Su,Radhika Muzumdar

Antiviral research 99:139-48 PubMed23648710

2013

Profiling of the kinome of cytomegalovirus-infected cells reveals the functional importance of host kinases Aurora A, ABL and AMPK.

Applications

Species

Human

Corina Hutterer,Sebastian Karl Wandinger,Sabrina Wagner,Regina Müller,Thomas Stamminger,Isabel Zeitträger,Klaus Godl,Roland Baumgartner,Stefan Strobl,Manfred Marschall

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ABL1 + ABL2 antibody [EPR1222(2)]

Flow Cytometry (Intracellular) - Anti-ABL1 + ABL2 antibody [EPR1222(2)] (AB134134)

Western blot - Anti-ABL1 + ABL2 antibody [EPR1222(2)] (AB134134)

OI-RD Scanning - Anti-ABL1 + ABL2 antibody [EPR1222(2)] (AB134134)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (8)

Product promise