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AB254341

Anti-ABL1 antibody [EPR23406-32]

  • 20ul selling size
  • KO Validated
  • RabMAb
  • Recombinant
  • What is this?

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Knockout Tested Rabbit Recombinant Monoclonal ABL1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

ABL, JTK7, ABL1, Tyrosine-protein kinase ABL1, Abelson murine leukemia viral oncogene homolog 1, Abelson tyrosine-protein kinase 1, Proto-oncogene c-Abl, p150

5 Images
Flow Cytometry (Intracellular) - Anti-ABL1 antibody [EPR23406-32] (AB254341)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ABL1 antibody [EPR23406-32] (AB254341)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized K-562 (Human chronic myelogenous leukemia lymphoblast) cells labelling ABL1 with ab254341 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Western blot - Anti-ABL1 antibody [EPR23406-32] (AB254341)
  • WB

Lab

Western blot - Anti-ABL1 antibody [EPR23406-32] (AB254341)

Lanes 1 - 3 : Merged signal (red and green). Green - ab254341 observed at 130 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa. ab254341 was shown to react with ABL1 in wild-type HeLa cells in western blot. The bands observed in ABL1 knockout cell line ab265612 (ABL1 knockout cell lysate ab263077) below 130 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. HeLa wild-type and ABL1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1 % Tween®) before incubation with ab254341 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 : 1000 dilution and a 1 : 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 : 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-ABL1 antibody [EPR23406-32] (ab254341) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ABL1 knockout HeLa cell lysate (<a href='/en-us/products/cell-lysates/human-abl1-knockout-hela-cell-lysate-ab263077'>ab263077</a>) at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Predicted band size: 122 kDa

Observed band size: 130 kDa

false

Western blot - Anti-ABL1 antibody [EPR23406-32] (AB254341)
  • WB

Lab

Western blot - Anti-ABL1 antibody [EPR23406-32] (AB254341)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Fresh lysates are recommended for loading to minimize target degradation.

Exposure time : 48 seconds.

All lanes:

Western blot - Anti-ABL1 antibody [EPR23406-32] (ab254341) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

Daudi (human burkitts lymphoma lymphoblast) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 122 kDa

Observed band size: 135 kDa

false

Western blot - Anti-ABL1 antibody [EPR23406-32] (AB254341)
  • WB

Lab

Western blot - Anti-ABL1 antibody [EPR23406-32] (AB254341)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 48 seconds.

All lanes:

Western blot - Anti-ABL1 antibody [EPR23406-32] (ab254341) at 1/1000 dilution

All lanes:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 122 kDa

Observed band size: 135 kDa,210 kDa

false

Western blot - Anti-ABL1 antibody [EPR23406-32] (AB254341)
  • WB

Lab

Western blot - Anti-ABL1 antibody [EPR23406-32] (AB254341)

Lanes 1 - 3 : Merged signal (red and green). Green - ab254341 observed at 130 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab254341 was shown to react with ABL1 in wild-type HeLa cells in western blot. The bands observed in ABL1 knockout cell line ab265612 (ABL1 knockout cell lysate ab263077) below 130 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. HeLa wild-type and ABL1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1 % Tween®) before incubation with ab254341 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 : 1000 dilution and a 1 : 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 : 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-ABL1 antibody [EPR23406-32] (ab254341) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ABL1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ABL1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-abl1-knockout-hela-cell-line-ab265612'>ab265612</a>)

Lane 3:

K562 cell lysate at 20 µg

Predicted band size: 122 kDa

Observed band size: 130 kDa

false

  • Carrier free

    Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23406-32

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABL1 also referred to as ABL-1 or ABL1 protein is a non-receptor tyrosine kinase with a mass of approximately 120 kDa. It is found throughout the body in diverse tissues with significant expression in the brain testes and hematopoietic cells. This protein consists of several functional domains including SH3 SH2 and a kinase domain that facilitate its interaction with various cellular components. ABL1 kinase plays a central role in cell differentiation division and stress response reflecting its mechanical versatility in cellular signaling.
Biological function summary

ABL1 functions by regulating key processes like cell cycle progression actin dynamics and cell adhesion. ABL1 participates as part of larger protein complexes that modulate cellular movement and gene transcription. When activated it phosphorylates a range of substrates that leads to various cellular outcomes. ABL1 operates in the cytoplasm and nucleus influencing both cytoskeletal rearrangement and DNA repair which highlights its critical function in maintaining cellular integrity and response to damage.

Pathways

ABL1 interacts in both the mitogenic and apoptotic pathways including involvement in the MAPK and PI3K/AKT pathways. ABL1 interfaces with proteins like CRK and GRB2 in these pathways integrating signals that determine cell fate decisions. Through its kinase activity ABL1 mediates signaling cascades that impact cellular growth and survival responding dynamically to internal and external cues.

ABL1 is notoriously implicated in chronic myeloid leukemia (CML) and acute lymphoblastic leukemia (ALL). The fusion protein BCR-ABL1 resulting from chromosomal translocation drives oncogenic signals that promote uncontrolled cell proliferation. The aberrant activity of BCR-ABL1 disrupts normal cellular regulation and interacts with proteins such as STAT5 enhancing leukemogenesis. Targeted therapies like tyrosine kinase inhibitors specifically hinder BCR-ABL1 activity demonstrating ABL1's importance in cancer pathology and treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9 (PubMed : 22810897). Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes. Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components. ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death. In response to oxidative stress, phosphorylates serine/threonine kinase PRKD2 at 'Tyr-717' (PubMed : 28428613). ABL1 is also translocated in the nucleus where it has DNA-binding activity and is involved in DNA-damage response and apoptosis. Many substrates are known mediators of DNA repair : DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52 or WRN. Activates the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates TP73, a primary regulator for this type of damage-induced apoptosis. Phosphorylates the caspase CASP9 on 'Tyr-153' and regulates its processing in the apoptotic response to DNA damage. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. ABL1 also acts as a regulator of multiple pathological signaling cascades during infection. Several known tyrosine-phosphorylated microbial proteins have been identified as ABL1 substrates. This is the case of A36R of Vaccinia virus, Tir (translocated intimin receptor) of pathogenic E.coli and possibly Citrobacter, CagA (cytotoxin-associated gene A) of H.pylori, or AnkA (ankyrin repeat-containing protein A) of A.phagocytophilum. Pathogens can highjack ABL1 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1. Regulates T-cell differentiation in a TBX21-dependent manner (By similarity). Positively regulates chemokine-mediated T-cell migration, polarization, and homing to lymph nodes and immune-challenged tissues, potentially via activation of NEDD9/HEF1 and RAP1 (By similarity). Phosphorylates TBX21 on tyrosine residues leading to an enhancement of its transcriptional activator activity (By similarity).
See full target information ABL1
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Product promise

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