Knockout Tested Rabbit Recombinant Monoclonal ABL1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (AB272701), expandable thumbnail](https://content.abcam.com/products/images/abl1-antibody-epr23406-32-bsa-and-azide-free-ab272701--western-blot-img164424.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (AB272701)")
![Western blot - Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (AB272701), expandable thumbnail](https://content.abcam.com/products/images/abl1-antibody-epr23406-32-bsa-and-azide-free-ab272701--western-blot-img418062.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (AB272701)")
![Flow Cytometry (Intracellular) - Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (AB272701), expandable thumbnail](https://content.abcam.com/products/images/abl1-antibody-epr23406-32-bsa-and-azide-free-ab272701--flow-cytometry-intracellular-img415640.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (AB272701)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | Flow Cyt | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Function
Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9 (PubMed:22810897). Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes. Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components. ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death. In response to oxidative stress, phosphorylates serine/threonine kinase PRKD2 at 'Tyr-717' (PubMed:28428613). ABL1 is also translocated in the nucleus where it has DNA-binding activity and is involved in DNA-damage response and apoptosis. Many substrates are known mediators of DNA repair: DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52 or WRN. Activates the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates TP73, a primary regulator for this type of damage-induced apoptosis. Phosphorylates the caspase CASP9 on 'Tyr-153' and regulates its processing in the apoptotic response to DNA damage. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. ABL1 acts also as a regulator of multiple pathological signaling cascades during infection. Several known tyrosine-phosphorylated microbial proteins have been identified as ABL1 substrates. This is the case of A36R of Vaccinia virus, Tir (translocated intimin receptor) of pathogenic E.coli and possibly Citrobacter, CagA (cytotoxin-associated gene A) of H.pylori, or AnkA (ankyrin repeat-containing protein A) of A.phagocytophilum. Pathogens can highjack ABL1 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1. Regulates T-cell differentiation in a TBX21-dependent manner (By similarity). Positively regulates chemokine-mediated T-cell migration, polarization, and homing to lymph nodes and immune-challenged tissues, potentially via activation of NEDD9/HEF1 and RAP1 (By similarity). Phosphorylates TBX21 on tyrosine residues leading to an enhancement of its transcriptional activator activity (By similarity).
Alternative names
ABL, JTK7, ABL1, Tyrosine-protein kinase ABL1, Abelson murine leukemia viral oncogene homolog 1, Abelson tyrosine-protein kinase 1, Proto-oncogene c-Abl, p150
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal ABL1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23406-32
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab272701 is the carrier-free version of Anti-ABL1 antibody [EPR23406-32] ab254341.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ABL1 also referred to as ABL-1 or ABL1 protein is a non-receptor tyrosine kinase with a mass of approximately 120 kDa. It is found throughout the body in diverse tissues with significant expression in the brain testes and hematopoietic cells. This protein consists of several functional domains including SH3 SH2 and a kinase domain that facilitate its interaction with various cellular components. ABL1 kinase plays a central role in cell differentiation division and stress response reflecting its mechanical versatility in cellular signaling.
- Biological function summary
ABL1 functions by regulating key processes like cell cycle progression actin dynamics and cell adhesion. ABL1 participates as part of larger protein complexes that modulate cellular movement and gene transcription. When activated it phosphorylates a range of substrates that leads to various cellular outcomes. ABL1 operates in the cytoplasm and nucleus influencing both cytoskeletal rearrangement and DNA repair which highlights its critical function in maintaining cellular integrity and response to damage.
- Pathways
ABL1 interacts in both the mitogenic and apoptotic pathways including involvement in the MAPK and PI3K/AKT pathways. ABL1 interfaces with proteins like CRK and GRB2 in these pathways integrating signals that determine cell fate decisions. Through its kinase activity ABL1 mediates signaling cascades that impact cellular growth and survival responding dynamically to internal and external cues.
- Associated diseases and disorders
ABL1 is notoriously implicated in chronic myeloid leukemia (CML) and acute lymphoblastic leukemia (ALL). The fusion protein BCR-ABL1 resulting from chromosomal translocation drives oncogenic signals that promote uncontrolled cell proliferation. The aberrant activity of BCR-ABL1 disrupts normal cellular regulation and interacts with proteins such as STAT5 enhancing leukemogenesis. Targeted therapies like tyrosine kinase inhibitors specifically hinder BCR-ABL1 activity demonstrating ABL1's importance in cancer pathology and treatment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (ab272701), expandable thumbnail](https://content.abcam.com/products/images/abl1-antibody-epr23406-32-bsa-and-azide-free-ab272701--western-blot-img164424.jpg "Western blot - Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (ab272701)")
Western blot - Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (ab272701)
This data was developed using the same antibody clone in a different buffer formulation (Anti-ABL1 antibody [EPR23406-32] ab254341).
Lanes 1 - 3: Merged signal (red and green). Green - Anti-ABL1 antibody [EPR23406-32] ab254341 observed at 130 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
Anti-ABL1 antibody [EPR23406-32] ab254341 was shown to react with ABL1 in wild-type HeLa cells in western blot. The bands observed in ABL1 knockout cell line Human ABL1 knockout HeLa cell line ab265612 (ABL1 knockout cell lysate Human ABL1 knockout HeLa cell lysate ab263077) below 130 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. HeLa wild-type and ABL1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1 % Tween®) before incubation with Anti-ABL1 antibody [EPR23406-32] ab254341 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1:1000 dilution and a 1:20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1:20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-ABL1 antibody [EPR23406-32] (Anti-ABL1 antibody [EPR23406-32] ab254341) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: ABL1 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human ABL1 knockout HeLa cell line (Human ABL1 knockout HeLa cell line ab265612)
Lane 3: K562 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 122 kDa
Observed band size: 130 kDa
![Western blot - Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (ab272701), expandable thumbnail](https://content.abcam.com/products/images/abl1-antibody-epr23406-32-bsa-and-azide-free-ab272701--western-blot-img418062.jpg "Western blot - Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (ab272701)")
Western blot - Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (ab272701)
This data was developed using the same antibody clone in a different buffer formulation (Anti-ABL1 antibody [EPR23406-32] ab254341). Lanes 1 - 3: Merged signal (red and green). Green - Anti-ABL1 antibody [EPR23406-32] ab254341 observed at 130 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa. Anti-ABL1 antibody [EPR23406-32] ab254341 was shown to react with ABL1 in wild-type HeLa cells in western blot. The bands observed in ABL1 knockout cell line Human ABL1 knockout HeLa cell line ab265612 (ABL1 knockout cell lysate Human ABL1 knockout HeLa cell lysate ab263077) below 130 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. HeLa wild-type and ABL1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1 % Tween®) before incubation with Anti-ABL1 antibody [EPR23406-32] ab254341 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1:1000 dilution and a 1:20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1:20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-ABL1 antibody [EPR23406-32] (Anti-ABL1 antibody [EPR23406-32] ab254341) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: ABL1 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human ABL1 knockout HeLa cell lysate (Human ABL1 knockout HeLa cell lysate ab263077)
Lane 2: Western blot - Human ABL1 knockout HeLa cell line (Human ABL1 knockout HeLa cell line ab277152)
Lane 3: K562 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 122 kDa
Observed band size: 130 kDa
![Flow Cytometry (Intracellular) - Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (ab272701), expandable thumbnail](https://content.abcam.com/products/images/abl1-antibody-epr23406-32-bsa-and-azide-free-ab272701--flow-cytometry-intracellular-img415640.jpg "Flow Cytometry (Intracellular) - Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (ab272701)")
Flow Cytometry (Intracellular) - Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (ab272701)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized K-562 (Human chronic myelogenous leukemia lymphoblast) cells labelling ABL1 with Anti-ABL1 antibody [EPR23406-32] ab254341 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ABL1 antibody [EPR23406-32] ab254341).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com