Rabbit Polyclonal ABL2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ABL2 aa 50-450.
Images
Publications
Filter by
- Journal of cell science 133:2020Abl-mediated PI3K activation regulates macrophage podosome formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuhuan Zhou et. al.PubMed 32393599
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ABL2 aa 50-450. The exact immunogen used to generate this antibody is proprietary information. Database link P42684
Consider this alternative
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Cow | Predicted |
| Zebrafish | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Zebrafish | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Non-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1. Positively regulates chemokine-mediated T-cell migration, polarization, and homing to lymph nodes and immune-challenged tissues, potentially via activation of NEDD9/HEF1 and RAP1 (By similarity).
Alternative names
ABLL, ARG, ABL2, Tyrosine-protein kinase ABL2, Abelson murine leukemia viral oncogene homolog 2, Abelson tyrosine-protein kinase 2, Abelson-related gene protein, Tyrosine-protein kinase ARG
Recommended products
Rabbit Polyclonal ABL2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ABL2 aa 50-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ABL2 aa 50-450. The exact immunogen used to generate this antibody is proprietary information. Database link P42684
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ABL2 also known as ARG (Abelson-related gene) is a non-receptor tyrosine kinase with a mass of approximately 120 kDa. It shares structural similarities with its relative ABL1 and is primarily localized in the cytoplasm and nucleus of cells. Expression of ABL2 occurs in various tissues including the brain where it plays significant roles in neuronal development. ABL2 functions mechanically by transferring a phosphate group from ATP to tyrosine residues on specific substrates which modulates the activity and function of these substrates.
- Biological function summary
ABL2 contributes to cellular processes such as actin cytoskeleton remodeling cell migration and adhesion. This protein interacts with different binding partners through its SH2 and SH3 domains implicating it in cellular signal transduction. ABL2 has been observed to occasionally form part of complexes that regulate these processes contributing to dynamic changes in cell morphology and motility.
- Pathways
ABL2 plays a significant role in the downstream signaling of receptor tyrosine kinases and integrins linking it to pathways like the PDGF and VEGF signaling pathways. ABL2 interacts with proteins such as CRK and C3G which influence cytoskeletal reorganization and cellular responses to external stimuli. These interactions highlight its role in conveying signals from the cell surface to internal signaling cascades.
- Associated diseases and disorders
ABL2 shows connections to various types of cancer including glioblastoma and chronic myeloid leukemia. Genetic alterations or dysregulation of ABL2 activity can lead to abnormal cellular proliferation and migration contributing to these malignancies. In particular the oncogenic fusion protein BCR-ABL derived from ABL1 is a well-known factor in leukemia and disruptions in ABL2 can similarly impact oncogenic signaling pathways. Research is ongoing to further understand ABL2’s involvement in tumorigenesis and its potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-ABL2 antibody (ab126256)
Gel concentration: 5%
All lanes: Western blot - Anti-ABL2 antibody (ab126256) at 1/1000 dilution
All lanes: IMR32 whole cell lysate at 30 µg
Predicted band size: 128 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com