AB140625

Anti-ABLIM1 antibody [EPR9508]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal ABLIM1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ABLIM, KIAA0059, LIMAB1, ABLIM1, Actin-binding LIM protein 1, abLIM-1, Actin-binding LIM protein family member 1, Actin-binding double zinc finger protein, Limatin

1 Images

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Western blot - Anti-ABLIM1 antibody [EPR9508] (AB140625)

All lanes:

Western blot - Anti-ABLIM1 antibody [EPR9508] (ab140625) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Fetal liver tissue lysate at 10 µg

Lane 3:

Fetal heart tissue lysate at 10 µg

Lane 4:

Y79 cell lysate at 10 µg

Predicted band size: 88 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9508

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABLIM1 also known as Actin-Binding LIM Protein 1 plays a role in the organization of the actin cytoskeleton. This protein has a molecular weight of approximately 75 kDa. ABLIM1 occurs in various tissues with high expression in the liver and pancreas. The protein contributes to cellular movement and structure by stabilizing actin filaments through its interaction with cytoskeletal components.

Biological function summary

ABLIM1 interacts with actin filaments to influence cell shape and motility. This protein can be part of a multiprotein complex containing actin-binding proteins that aid in cytoskeletal rearrangements. ABLIM1 through its actin-stabilizing function plays a significant role in intracellular signaling processes that rely on structural integrity and mechanotransduction which are vital in maintaining cellular architecture and function.

Pathways

ABLIM1’s function is particularly critical in the Rho GTPase pathway and the focal adhesion pathway. Through these pathways ABLIM1 interacts with proteins such as vinculin and talin which mediate signal transduction from the extracellular matrix to the actin cytoskeleton. These interactions help in the regulation of cell adhesion migration and mechanical responses to external stimuli.

ABLIM1 has been implicated in certain cancers and neurodegenerative disorders. In cancer alterations in its expression can affect cell migration and invasion by interacting with proteins like integrins which are important in tumor metastasis. Neurodegenerative disorders may involve ABLIM1 through pathways impacting synaptic stability and plasticity linking it to proteins like α-synuclein known for its role in diseases such as Parkinson’s.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act as scaffold protein (By similarity). May play a role in the development of the retina. Has been suggested to play a role in axon guidance.

See full target information ABLIM1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

In vivo (Athens, Greece) 38:399-408 PubMed38148084

2023

Implication of Amyloid Precursor-like Protein 2 Expression in Cutaneous Squamous Cell Carcinoma Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodi Huang,Jihye Yang,Haoran Xi,Meilan Zhang,Yeongjoo Oh,Zhehu Jin,Zhenlong Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ABLIM1 antibody [EPR9508]

Western blot - Anti-ABLIM1 antibody [EPR9508] (AB140625)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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