Mouse Monoclonal ABO antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human Histo-blood group ABO system transferase.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABO antibody [33C13] - BSA and Azide free (AB212418), expandable thumbnail](https://content.abcam.com/products/images/abo-antibody-33c13-bsa-and-azide-free-ab212418--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84707.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABO antibody [33C13] - BSA and Azide free (AB212418)")
Publications
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- Immunobiology 227:1522732022Prevalence of high affinity naturally occurring IgG2 antibodies against human chorionic gonadotropin and its subunits in patients with ovarian cyst.Applications:Unspecified applicationReactive species:Unspecified reactive speciesN Chikadze,M Tevzadze,M Janelidze,P Lydyard,N PorakishviliPubMed 36088866
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Native Full Length Protein corresponding to Human Histo-blood group ABO system transferase. Database link P16442
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. Glycosyltransferase that catalyzes the transfer of carbohydrates to H antigen, forming the antigenic structures of the ABO blood group.
Alternative names
Histo-blood group ABO system transferase, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Histo-blood group A transferase, Histo-blood group B transferase, NAGAT, A transferase, B transferase, ABO
Recommended products
Mouse Monoclonal ABO antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human Histo-blood group ABO system transferase.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Native Full Length Protein corresponding to Human Histo-blood group ABO system transferase. Database link P16442
- Carrier free
- Yes
- Clone number
- 33C13
- Purification technique
- Affinity purification Protein A/G
- Specificity
ab212418 preferably reacts with determinants of chain A and H type 3 (Gal1-3GalNAc-R) and 4 (Gal1-3GalNAc-R), but not with type 1 and 2 chain structures. It is not reactive with immuno-dominant A trisaccharide.
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from Bioreactor Concentrate
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ABO blood group system frequently referred to as ABO is a critical component in determining blood type. It involves the presence of antigens on the surface of red blood cells which are glycosyltransferase enzymes that transfer sugar residues onto the H antigen. The gene responsible for this system is located on chromosome 9 and the variations in the gene produce the A B and O blood types. These glycoproteins and glycolipids confer blood group specificity. ABO is found in the erythrocytic lineage and is an integral part of the blood plasma context.
- Biological function summary
The ABO proteins modulate immune response mechanisms. They participate in the formation of specific antigens and antibodies that define the ABO blood group system. These proteins are not part of a larger complex but interact closely with components in the immune system. In individuals the presence or absence of antigen types influences immune activity and the antigen-antibody structure is essential in transfusion medicine.
- Pathways
Researchers link the ABO system with several immune response pathways. The system integrates into the complement system and humoral immunity affecting compatibility in blood transfusions. It is indirectly related to proteins in the complement cascade and these interactions facilitate or hinder immune reactions during transfusions and organ transplants. The pathway involvement highlights the system’s significance in cellular communication and immune mobilization.
- Associated diseases and disorders
ABO incompatibility can lead to hemolytic disease of the newborn and transfusion reactions. In such contexts the presence of incompatible ABO antigens triggers immune responses resulting in adverse events. It also indirectly connects to diseases like gastric cancer where blood type prevalence indicates varying disease risk profiles. The interaction with proteins such as the Rh factor can exacerbate these conditions showing how ABO status affects susceptibility and symptom severity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABO antibody [33C13] - BSA and Azide free (ab212418), expandable thumbnail](https://content.abcam.com/products/images/abo-antibody-33c13-bsa-and-azide-free-ab212418--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84707.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABO antibody [33C13] - BSA and Azide free (ab212418)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABO antibody [33C13] - BSA and Azide free (ab212418)
Immunohistochemical analysis of formalin fixed paraffin embedded Human colon carcinoma labeling Blood Group H2 Antigen with ab212418 at 1 μg/ml.
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