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AB272286

Anti-ABO antibody [EPR22582-261] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal ABO antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Histo-blood group ABO system transferase, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Histo-blood group A transferase, Histo-blood group B transferase, NAGAT, A transferase, B transferase, ABO

1 Images
Sandwich ELISA - Anti-ABO antibody [EPR22582-261] - BSA and Azide free (Detector) (AB272286)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-ABO antibody [EPR22582-261] - BSA and Azide free (Detector) (AB272286)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab270207).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22582-261

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab272286 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ABO. The recommended pair for sandwich ELISA is:
Capture: ab272285, Human ABO Capture Antibody (unconjugated)
Detector: ab272286, Human ABO Detector Antibody (unconjugated)
The reference range value is 31.25 - 2000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ABO blood group system frequently referred to as ABO is a critical component in determining blood type. It involves the presence of antigens on the surface of red blood cells which are glycosyltransferase enzymes that transfer sugar residues onto the H antigen. The gene responsible for this system is located on chromosome 9 and the variations in the gene produce the A B and O blood types. These glycoproteins and glycolipids confer blood group specificity. ABO is found in the erythrocytic lineage and is an integral part of the blood plasma context.
Biological function summary

The ABO proteins modulate immune response mechanisms. They participate in the formation of specific antigens and antibodies that define the ABO blood group system. These proteins are not part of a larger complex but interact closely with components in the immune system. In individuals the presence or absence of antigen types influences immune activity and the antigen-antibody structure is essential in transfusion medicine.

Pathways

Researchers link the ABO system with several immune response pathways. The system integrates into the complement system and humoral immunity affecting compatibility in blood transfusions. It is indirectly related to proteins in the complement cascade and these interactions facilitate or hinder immune reactions during transfusions and organ transplants. The pathway involvement highlights the system’s significance in cellular communication and immune mobilization.

ABO incompatibility can lead to hemolytic disease of the newborn and transfusion reactions. In such contexts the presence of incompatible ABO antigens triggers immune responses resulting in adverse events. It also indirectly connects to diseases like gastric cancer where blood type prevalence indicates varying disease risk profiles. The interaction with proteins such as the Rh factor can exacerbate these conditions showing how ABO status affects susceptibility and symptom severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens : A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.. Glycosyltransferase that catalyzes the transfer of carbohydrates to H antigen, forming the antigenic structures of the ABO blood group.
See full target information Histo-blood group ABO system transferase
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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