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Rabbit Recombinant Monoclonal ABP1 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-ABP1 antibody [EPR24299-52] (AB278497), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABP1 antibody [EPR24299-52] (AB278497), expandable thumbnail
  • Western blot - Anti-ABP1 antibody [EPR24299-52] (AB278497), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABP1 antibody [EPR24299-52] (AB278497), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABP1 antibody [EPR24299-52] (AB278497), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBFlow Cyt (Intra)IHC-PIHC-Fr
Human
Not recommended
Not recommended
Tested
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

-

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Target data

Function

Catalyzes the oxidative deamination of primary amines to the corresponding aldehydes with the concomitant production of hydrogen peroxide and ammonia (PubMed:12072962, PubMed:19764817, PubMed:239684, PubMed:8144586). Its preferred substrates are the diamines histamine and 1-methylhistamine and it could therefore play a role in allergic and immune responses (PubMed:12072962). Has a broad specificity for diamines and can also act on cadaverine and putrescine, two products of amino acid catabolism (PubMed:12072962). It could also act on polyamines, like spermidine and spermine though less efficiently, and regulate various biological processes (PubMed:12072962, PubMed:239684).

Alternative names

ABP, ABP1, DAO, DAO1, AOC1, Diamine oxidase [copper-containing], Diamine oxidase, Amiloride-binding protein, Amiloride-binding protein 1, Amine oxidase copper domain-containing protein 1, Histaminase, Kidney amine oxidase, KAO, KDAO

Recommended products

Rabbit Recombinant Monoclonal ABP1 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24299-52
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ABP1 also known as Auxin Binding Protein 1 is a protein with a molecular mass of approximately 22 kDa. This protein primarily functions as an auxin receptor in plants mediating various cellular responses to the plant hormone auxin. ABP1 locates mainly in the endoplasmic reticulum and is present across various plant tissues. The protein has a significant affinity for auxin and triggers specific intracellular signaling cascades essential for growth and development processes.

Biological function summary

ABP1 plays an important role in controlling cell elongation and division and it works in tandem with other auxin transporters. The protein functions within a broader receptor complex interacting with other proteins to modify plant cell physiology. Its action facilitates cell wall modification which directly influences the plant's response to environmental and endogenous signals. Through these interactions ABP1 significantly contributes to regulating the plant's phototropic and gravitropic responses.

Pathways

The operation of ABP1 integrates into the auxin signaling pathway and is associated with the regulation of gene expression. It links to TIR1 an important F-box protein in the SCF complex involved in the auxin signal transduction pathway. Together ABP1 and TIR1 coordinate to modulate the stability of transcription factors affecting downstream gene expression necessary for plant growth and development.

Associated diseases and disorders

ABP1 itself mostly pertains to plant biology but its function offers insights into plant growth abnormalities. Disruptions in ABP1 function may result in disorders like auxin-insensitive phenotypes leading to defective root and shoot development. Additionally ABP1's interaction with PIN proteins responsible for auxin efflux creates potential for research into correcting growth defects in crops making it a critical focus in agricultural science.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-ABP1 antibody [EPR24299-52] (ab278497), expandable thumbnail

    Western blot - Anti-ABP1 antibody [EPR24299-52] (ab278497)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile observed is consistent with what has been described in the literature (PMID:25037221, 12072962).

    Negative control: human hypothalamus (PMID:12072962).

    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure time: Lane 1: 15 seconds; Lanes 2-4: 103 seconds.

    All lanes: Western blot - Anti-ABP1 antibody [EPR24299-52] (ab278497) at 1/1000 dilution

    Lane 1: Human kidney tissue lysate at 20 µg

    Lane 2: Human colon tissue lysate at 20 µg

    Lane 3: Human stomach tissue lysate at 20 µg

    Lane 4: Human hypothalamus tissue lysate at 20 µg

    Secondary

    All lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/2000 dilution

    Predicted band size: 85 kDa

    Observed band size: 85 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABP1 antibody [EPR24299-52] (ab278497), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABP1 antibody [EPR24299-52] (ab278497)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling ABP1 with ab278497 at 1/2000 dilution (0.248 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on human placenta (PMID:12072962). The section was incubated with ab278497 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-ABP1 antibody [EPR24299-52] (ab278497), expandable thumbnail

    Western blot - Anti-ABP1 antibody [EPR24299-52] (ab278497)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Sample is non-boiled as boiling may cause protein aggregates.

    Exposure time: 37 seconds

    All lanes: Western blot - Anti-ABP1 antibody [EPR24299-52] (ab278497) at 1/1000 dilution

    All lanes: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 85 kDa

    Observed band size: 85 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABP1 antibody [EPR24299-52] (ab278497), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABP1 antibody [EPR24299-52] (ab278497)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling ABP1 with ab278497 at 1/2000 dilution (0.248 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on human kidney (PMID:12072962). The section was incubated with ab278497 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABP1 antibody [EPR24299-52] (ab278497), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABP1 antibody [EPR24299-52] (ab278497)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling ABP1 with ab278497 at 1/2000 dilution (0.248 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Negative control: No staining on human cerebrum (PMID:12072962). The section was incubated with ab278497 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Flow Cytometry (Intracellular) - Anti-ABP1 antibody [EPR24299-52] (ab278497), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ABP1 antibody [EPR24299-52] (ab278497)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) cells labelling ABP1 with ab278497 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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