Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free (AB278501)

This data was developed using ab278497, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling ABP1 with ab278497 at 1/2000 dilution (0.248 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on human placenta (PMID : 12072962). The section was incubated with ab278497 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free (AB278501)

This data was developed using ab278497, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling ABP1 with ab278497 at 1/2000 dilution (0.248 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Negative control : No staining on human cerebrum (PMID : 12072962). The section was incubated with ab278497 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free (AB278501)

This data was developed using ab278497, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling ABP1 with ab278497 at 1/2000 dilution (0.248 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on human kidney (PMID : 12072962). The section was incubated with ab278497 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free (AB278501)

This data was developed using ab278497, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) cells labelling ABP1 with ab278497 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• WB

Lab

Western blot - Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free (AB278501)

This data was developed using ab278497, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Sample is non-boiled as boiling may cause protein aggregates.

Exposure time : 37 seconds

All lanes:

Western blot - Anti-ABP1 antibody [EPR24299-52] (<a href='/en-us/products/primary-antibodies/abp1-antibody-epr24299-52-ab278497'>ab278497</a>) at 1/1000 dilution

All lanes:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 85 kDa

Observed band size: 85 kDa

false

• WB

Lab

Western blot - Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free (AB278501)

This data was developed using ab278497, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile observed is consistent with what has been described in the literature (PMID : 25037221, 12072962).

Negative control : human hypothalamus (PMID : 12072962).

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time : Lane 1 : 15 seconds; Lanes 2-4 : 103 seconds.

All lanes:

Western blot - Anti-ABP1 antibody [EPR24299-52] (<a href='/en-us/products/primary-antibodies/abp1-antibody-epr24299-52-ab278497'>ab278497</a>) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

Human colon tissue lysate at 20 µg

Lane 3:

Human stomach tissue lysate at 20 µg

Lane 4:

Human hypothalamus tissue lysate at 20 µg

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/2000 dilution

Predicted band size: 85 kDa

Observed band size: 85 kDa

false