AB199157

Anti-AC3 antibody - C-terminal

Name: Anti-AC3 antibody - C-terminal (ab199157) | Abcam
Brand: Abcam Limited
SKU: AB199157
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal AC3 antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ADCY3.

View Alternative Names

KIAA0511, ADCY3, Adenylate cyclase type 3, ATP pyrophosphate-lyase 3, Adenylate cyclase type III, Adenylyl cyclase 3, AC-III, AC3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AC3 antibody - C-terminal (AB199157)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AC3 antibody - C-terminal (AB199157)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling AC3 with ab199157 at 1/50 dilution.

Supplier Data

Western blot - Anti-AC3 antibody - C-terminal (AB199157)

Gel : 6% SDS-PAGE

All lanes:

Western blot - Anti-AC3 antibody - C-terminal (ab199157) at 1/1000 dilution

Lane 1:

Raji cell lysate at 40 µg

Lane 2:

T98G cell lysate at 40 µg

Lane 3:

Mouse brain tissue lysate at 40 µg

Lane 4:

Rat brain tissue lysate at 40 µg

Secondary

All lanes:

HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ADCY3. The exact immunogen used to generate this antibody is proprietary information.

O60266

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein AC3 also known as 'Adcy3' functions as an adenylate cyclase enzyme. This enzyme plays a significant mechanical role by converting ATP (adenosine triphosphate) to cyclic AMP (cAMP) which acts as a secondary messenger in various signaling pathways. It is also referred to as '1a12' in some studies. Weighing approximately 137 kDa AC3 is mainly expressed in the olfactory epithelium where it contributes to odor detection the brain and other tissues at lower levels. This protein operates at the cell membrane where it interacts with G proteins to mediate signal transduction processes.

Biological function summary

Adenylate cyclase 3 (AC3) serves as an essential component in sensory signal transduction and is part of the adenylyl cyclase complex. It plays an important role in the conversion of external stimuli into intracellular signals important for processes like olfaction and hormonal response. Within neurons AC3 influences synaptic plasticity and learning by regulating cAMP levels. This modulation affects various neural activities and responses making AC3 important for central nervous system functions.

Pathways

AC3 is integrally involved in the G-protein coupled receptor (GPCR) signaling pathways. These pathways involve the activation of AC3 by G-proteins leading to enhanced production of cAMP which subsequently activates protein kinase A (PKA). AC3 collaborates with other adenylate cyclases and G-protein subunits such as Gα and Gβγ within these pathways. This intricate signaling cascade influences cellular responses to hormones and neurotransmitters and alters gene expression through cAMP-mediated pathways.

AC3 has associations with conditions like obesity and depression. Abnormalities in AC3 function or expression can lead to impaired cAMP signaling disrupting normal metabolic and neurological processes. Research indicates that AC3 interacts with the melanocortin-4 receptor (MC4R) in regulating energy homeostasis and body weight linking it to obesity. In depression altered AC3 levels impact neurotransmitter signaling contributing to mood disorders. Understanding AC3's role in these conditions may offer therapeutic insights into disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling. Participates in signaling cascades triggered by odorant receptors via its function in cAMP biosynthesis : specifically activated by G alpha protein GNAL/G(olf) in olfactory epithelium. Required for normal sperm motility and normal male fertility. Plays a role in regulating insulin levels and body fat accumulation in response to a high fat diet.

See full target information ADCY3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 299:105128 PubMed37543361

2023

Brain-specific glycosylation enzyme GnT-IX maintains levels of protein tyrosine phosphatase receptor PTPRZ, thereby mediating glioma growth.

Applications

Unspecified application

Species

Unspecified reactive species

Kenichiro Nagai,Yui Muto,Saori Miura,Kazuto Takahashi,Yu Naruse,Ryo Hiruta,Yuko Hashimoto,Miwa Uzuki,Yoshimi Haga,Risa Fujii,Koji Ueda,Yasushi Kawaguchi,Masazumi Fujii,Shinobu Kitazume

Genome biology 24:59 PubMed36991492

2023

CRISPR screens identify gene targets at breast cancer risk loci.

Applications

Unspecified application

Species

Unspecified reactive species

Natasha K Tuano,Jonathan Beesley,Murray Manning,Wei Shi,Laura Perlaza-Jimenez,Luis F Malaver-Ortega,Jacob M Paynter,Debra Black,Andrew Civitarese,Karen McCue,Aaron Hatzipantelis,Kristine Hillman,Susanne Kaufmann,Haran Sivakumaran,Jose M Polo,Roger R Reddel,Vimla Band,Juliet D French,Stacey L Edwards,David R Powell,Georgia Chenevix-Trench,Joseph Rosenbluh

American journal of physiology. Gastrointestinal and liver physiology 318:G10-G22 PubMed31433213

2019

LINC00319 acts as a sponge to accelerate tumor growth and metastasis in gastric cancer by upregulating .

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zou,Kun Wu,Chao Lin,Zhi-Gang Jie

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-AC3 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AC3 antibody - C-terminal (AB199157)

Western blot - Anti-AC3 antibody - C-terminal (AB199157)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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