AB66037

Anti-AC5 antibody

Name: Anti-AC5 antibody (ab66037) | Abcam
Brand: Abcam Limited
SKU: AB66037
Price: 525 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal AC5 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human Adenylate cyclase type 5.

View Alternative Names

Adenylate cyclase type 5, ATP pyrophosphate-lyase 5, Adenylate cyclase type V, Adenylyl cyclase 5, AC5, ADCY5

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-AC5 antibody (AB66037)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-AC5 antibody (AB66037)

ICC/IF image of ab66037 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66037, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-AC5 antibody (AB66037)

All lanes:

Western blot - Anti-AC5 antibody (ab66037) at 1/500 dilution

Lane 1:

Extracts from COLO205 cells at 30 µg with no immunising peptide

Lane 2:

Extracts from COLO205 cells at 30 µg with immunising peptide

Predicted band size: 139 kDa

Observed band size: 139 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human Adenylate cyclase type 5. The exact immunogen used to generate this antibody is proprietary information.

O95622

Specificity

This antibody can cross-react with ADCY6.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adenylate Cyclase 5 (AC5) is an enzyme that plays an essential role in catalyzing the conversion of ATP to cyclic AMP (cAMP). AC5 is also known as ADCY5. Its molecular weight is approximately 150 kDa. AC5 is mostly expressed in the brain and heart tissues suggesting its significant role in central and cardiovascular functions.

Biological function summary

AC5 contributes to the regulation of cAMP levels an important secondary messenger in many signaling cascades. AC5 forms part of enzyme complexes that modulate cellular responses through cAMP signaling pathways. Alteration in its activity can directly impact the regulation of neurotransmitter and hormone activity influencing processes such as learning memory and myocardial contractility.

Pathways

AC5 integrates into the cAMP-dependent signaling pathway and the adrenergic signaling pathway. It associates with other proteins like G protein-coupled receptors (GPCRs) and protein kinase A (PKA) that enhance these pathways' functions. Through its role in these pathways AC5 influences diverse physiological responses such as cardiac rhythm and neural transmission.

AC5 has a significant connection to heart failure and movement disorders such as dyskinesia. Heart failure relates to AC5 through its regulation of cardiac contractility where disruption in cAMP signaling can worsen the condition. In movement disorders like dyskinesia AC5’s connection to dopamine receptors alters the sensitivity of pathways in the brain influencing motor control and stability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling (PubMed : 15385642, PubMed : 24700542, PubMed : 26206488). Mediates signaling downstream of ADRB1 (PubMed : 24700542). Regulates the increase of free cytosolic Ca(2+) in response to increased blood glucose levels and contributes to the regulation of Ca(2+)-dependent insulin secretion (PubMed : 24740569).

See full target information Adenylate cyclase type 5

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40004085

2025

ADCY5 Gene Affects Seasonal Reproduction in Dairy Goats by Regulating Ovarian Granulosa Cells Steroid Hormone Synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chenbo Shi,Fuhong Zhang,Qiuya He,Jianjun Man,Yuanpan Mu,Jianqing Zhao,Lu Zhu,Juan J Loor,Jun Luo

Theranostics 11:5650-5674 PubMed33897873

2021

Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhang,Keke Yu,Lifang Ma,Zijun Qian,Xiaoting Tian,Yayou Miao,Yongjie Niu,Xin Xu,Susu Guo,Yueyue Yang,Zhixian Wang,Xiangfei Xue,Chuanjia Gu,Wentao Fang,Jiayuan Sun,Yongchun Yu,Jiayi Wang

eLife 9: PubMed32894219

2020

Novel C1q receptor-mediated signaling controls neural stem cell behavior and neurorepair.

Applications

Unspecified application

Species

Unspecified reactive species

Francisca Benavente,Katja M Piltti,Mitra J Hooshmand,Aileen A Nava,Anita Lakatos,Brianna G Feld,Dana Creasman,Paul D Gershon,Aileen Anderson

Cell death and differentiation 26:1195-1212 PubMed30209302

2018

Caveolin-1 impairs PKA-DRP1-mediated remodelling of ER-mitochondria communication during the early phase of ER stress.

Applications

Unspecified application

Species

Unspecified reactive species

Roberto Bravo-Sagua,Valentina Parra,Carolina Ortiz-Sandoval,Mario Navarro-Marquez,Andrea E Rodríguez,Natalia Diaz-Valdivia,Carlos Sanhueza,Camila Lopez-Crisosto,Nasser Tahbaz,Beverly A Rothermel,Joseph A Hill,Mariana Cifuentes,Thomas Simmen,Andrew F G Quest,Sergio Lavandero

Pflugers Archiv : European journal of physiology 470:1705-1717 PubMed30094477

2018

Adenylyl cyclase 6 is involved in the hyposecretory status of experimental colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Romero-Calvo,Borja Ocón,Reyes Gámez-Belmonte,Cristina Hernández-Chirlaque,Hugo R de Jonge,Marcel J Bijvelds,Olga Martínez-Augustin,Fermín Sánchez de Medina

Diabetes 63:3009-21 PubMed24740569

2014

ADCY5 couples glucose to insulin secretion in human islets.

Applications

IHC

Species

Unspecified reactive species

David J Hodson,Ryan K Mitchell,Lorella Marselli,Timothy J Pullen,Silvia Gimeno Brias,Francesca Semplici,Katy L Everett,Dermot M F Cooper,Marco Bugliani,Piero Marchetti,Vanessa Lavallard,Domenico Bosco,Lorenzo Piemonti,Paul R Johnson,Stephen J Hughes,Daliang Li,Wen-Hong Li,A M James Shapiro,Guy A Rutter

Journal of cell science 125:176-88 PubMed22250198

2012

Adenylate cyclase 5 coordinates the action of ADP, P2Y1, P2Y13 and ATP-gated P2X7 receptors on axonal elongation.

Applications

Unspecified application

Species

Mouse

Ana del Puerto,Juan-Ignacio Díaz-Hernández,Mónica Tapia,Rosa Gomez-Villafuertes,María José Benitez,Jin Zhang,María Teresa Miras-Portugal,Francisco Wandosell,Miguel Díaz-Hernández,Juan José Garrido

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-AC5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-AC5 antibody (AB66037)

Western blot - Anti-AC5 antibody (AB66037)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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Secondary Antibodies

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