AB327110

Anti-ACAA1 Antibody [CL2660]

Name: Anti-ACAA1 Antibody [CL2660] (ab327110) | Abcam
Brand: Abcam Limited
SKU: AB327110
Price: 600 USD
Availability: InStock

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Mouse Monoclonal ACAA1/Beta-ketothiolase antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ACAA1.

View Alternative Names

ACAA, PTHIO, ACAA1, Acetyl-CoA C-myristoyltransferase, Acetyl-CoA acyltransferase, Beta-ketothiolase, Peroxisomal 3-oxoacyl-CoA thiolase

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1 Antibody [CL2660] (AB327110)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1 Antibody [CL2660] (AB327110)

Immunohistochemical staining of human skeletal muscle using ab327110 at 1 : 500–1 : 1000 dilution, showing granular cytoplasmic immunoreactivity.

Immunocytochemistry/ Immunofluorescence - Anti-ACAA1 Antibody [CL2660] (AB327110)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ACAA1 Antibody [CL2660] (AB327110)

Immunofluorescence staining of CACO-2 cells using ab327110 at 2–10 µg/ml, showing specific staining in peroxisomes in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1 Antibody [CL2660] (AB327110)

Immunohistochemical staining of human prostate using ab327110 at 1 : 500–1 : 1000 dilution, showing granular cytoplasmic immunoreactivity in glandular cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1 Antibody [CL2660] (AB327110)

Immunohistochemical staining of human small intestine using ab327110 at 1 : 500–1 : 1000 dilution, showing strong granular cytoplasmic positivity in glandular cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1 Antibody [CL2660] (AB327110)

Immunohistochemical staining of human liver using ab327110 at 1 : 500–1 : 1000 dilution, showing strong granular cytoplasmic positivity in hepatocytes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1 Antibody [CL2660] (AB327110)

Immunohistochemical staining of human kidney using ab327110 at 1 : 500–1 : 1000 dilution, showing granular cytoplasmic immunoreactivity in renal tubules.

Supplier Data

Western blot - Anti-ACAA1 Antibody [CL2660] (AB327110)

Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using ab327110 at 1 µg/ml. Remaining relative intensity is presented. Loading control : Anti-PPIB.

All lanes:

Western blot - Anti-ACAA1 Antibody [CL2660] (ab327110)

Lane 2:

U-251MG cells transfected with control siRNA

Lane 3:

U-251MG cells transfected with target specific siRNA probe #1

Lane 4:

U-251MG cells transfected with target specific siRNA probe #2

Predicted band size: 44.29 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2660

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ACAA1.

P09110

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Responsible for the thiolytic cleavage of straight chain 3-keto fatty acyl-CoAs (3-oxoacyl-CoAs) (PubMed : 11734571, PubMed : 2882519). Plays an important role in fatty acid peroxisomal beta-oxidation (PubMed : 11734571, PubMed : 2882519). Catalyzes the cleavage of short, medium, long, and very long straight chain 3-oxoacyl-CoAs (PubMed : 11734571, PubMed : 2882519).

See full target information ACAA1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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