AB110289

Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7]

Name: Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289) | Abcam
Brand: Abcam Limited
SKU: AB110289

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(5 Publications)

Mouse Monoclonal ACAA1/Beta-ketothiolase antibody. Suitable for ICC, IP, Flow Cyt, WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

ACAA, PTHIO, ACAA1, Acetyl-CoA C-myristoyltransferase, Acetyl-CoA acyltransferase, Beta-ketothiolase, Peroxisomal 3-oxoacyl-CoA thiolase

4 Images

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Immunocytochemistry - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (AB110289)

ab110289, at 5 μg/mL, staining ACAA1/Beta-ketothiolase in Human HepG2 cells. DAPI was used to stain the cell nuclei (blue).

Flow Cytometry - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (AB110289)

Flow Cyt

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Flow Cytometry - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (AB110289)

ab110289, at 1 μg/mL, staining ACAA1/Beta-ketothiolase in HL-60 cells (blue) or using an isotype control (red).

Lab

Immunoprecipitation - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (AB110289)

ACAA1/Beta-ketothiolase was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5μg of Mouse monoclonal to ACAA1/Beta-ketothiolase and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).

The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70°C; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab110289.

Secondary : Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

Band : 44kDa; ACAA1/Beta-ketothiolase

All lanes:

Immunoprecipitation - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)

Predicted band size: 44 kDa

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Exposure time: 4min

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Western blot - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (AB110289)

All lanes:

Western blot - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289) at 1 µg/mL

Lane 1:

Human liver at 10 µg

Lane 2:

HepG2 at 10 µg

Lane 3:

Rat liver at 10 µg

Lane 4:

Mouse liver at 10 µg

Predicted band size: 44 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2H6BE7

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC, Flow Cyt, IP, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "5 µg/mL", "ICC-species-notes": "Heat-induced antigen retrieval improves signal", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5 µg/mL", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Precipitation Ammonium Sulphate

Purification notes

Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACAA1 also known as Beta-ketothiolase is an enzyme involved in key metabolic processes. This enzyme with a molecular mass of approximately 41 kDa plays a pivotal role in the thiolytic cleavage of 3-ketoacyl-CoA an essential step in both fatty acid beta-oxidation and ketogenesis. ACAA1 is expressed in various tissues notably liver and muscle where high rates of fatty acid metabolism occur. As part of the peroxisome it functions effectively to maintain cellular lipid homeostasis.

Biological function summary

ACAA1 facilitates the metabolic breakdown of fatty acids by participating in the final steps of beta-oxidation. It does not form part of a larger enzymatic complex but it operates alongside enzymes like acyl-CoA oxidase and enoyl-CoA hydratase that contribute to the fatty acid oxidation pathway. Through its enzymatic activity ACAA1 directly influences energy production and metabolic rate which are important for maintaining cellular energy balance.

Pathways

Beta-ketothiolase plays an integral role in the fatty acid beta-oxidation and ketogenesis pathways. These pathways are critical for the breakdown of fatty acids into acetyl-CoA which enters the citric acid cycle for energy production. ACAA1 works closely with enzymes such as acetoacetyl-CoA thiolase and HMG-CoA lyase in these processes highlighting its involvement in converting fatty acids into usable energy forms.

Malfunction or deficiency in ACAA1 can lead to issues like ketoacidosis and metabolic dysregulation disorders. The enzyme's dysfunction can result in improper fatty acid metabolism and accumulation of toxic intermediates. ACAA1 shares a connection with HMG-CoA synthase in ketogenesis and any disruption in these pathways may contribute to metabolic diseases impacting energy homeostasis and leading to serious health conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Responsible for the thiolytic cleavage of straight chain 3-keto fatty acyl-CoAs (3-oxoacyl-CoAs) (PubMed : 11734571, PubMed : 2882519). Plays an important role in fatty acid peroxisomal beta-oxidation (PubMed : 11734571, PubMed : 2882519). Catalyzes the cleavage of short, medium, long, and very long straight chain 3-oxoacyl-CoAs (PubMed : 11734571, PubMed : 2882519).

See full target information ACAA1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Foods (Basel, Switzerland) 11: PubMed36010422

2022

Betaine Reduces Lipid Anabolism and Promotes Lipid Transport in Mice Fed a High-Fat Diet by Influencing Intestinal Protein Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Hu,Lun Tan,Xiaojiao Li,Jingjing Li,Caiyun Fan,Feng Huang,Zhao Zhuo,Kun Hou,Yinying Xu,Qingfeng Wang,Yongxin Yang,Jianbo Cheng

Molecular and cellular neurosciences 107:103536 PubMed32777345

2020

Aging lowers PEX5 levels in cortical neurons in male and female mouse brains.

Applications

Unspecified application

Species

Unspecified reactive species

Ndidi-Ese Uzor,Diego Morales Scheihing,Gab Seok Kim,Jose Felix Moruno-Manchon,Liang Zhu,Caroline R Reynolds,Jessica M Stephenson,Aleah Holmes,Louise D McCullough,Andrey S Tsvetkov

FASEB journal : official publication of the Federa 33:9990-10004 PubMed31167079

2019

Maternal undernutrition induces fetal hepatic lipid metabolism disorder and affects the development of fetal liver in a sheep model.

Applications

Unspecified application

Species

Unspecified reactive species

Yanfeng Xue,Changzheng Guo,Fan Hu,Weiyun Zhu,Shengyong Mao

Oncotarget 8:104508-104524 PubMed29262657

2017

hsa-miR-29c-3p regulates biological function of colorectal cancer by targeting SPARC.

Applications

Unspecified application

Species

Unspecified reactive species

Shitong Zhang,Jianjun Jin,Xiaoxiao Tian,Lijuan Wu

Aging cell 13:1049-58 PubMed25257068

2014

Shc depletion stimulates brown fat activity in vivo and in vitro.

Applications

Unspecified application

Species

Mouse

Alexey Tomilov,Ahmed Bettaieb,Kyoungmi Kim,Sunil Sahdeo,Natalia Tomilova,Adam Lam,Kevork Hagopian,Michelle Connell,Jennifer Fong,Douglas Rowland,Stephen Griffey,Jon Ramsey,Fawaz Haj,Gino Cortopassi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7]

Immunocytochemistry - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (AB110289)

Flow Cytometry - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (AB110289)

Immunoprecipitation - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (AB110289)

Western blot - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (AB110289)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise