AB154091

Anti-ACAA1/Beta-ketothiolase antibody

Name: Anti-ACAA1/Beta-ketothiolase antibody (ab154091) | Abcam
Brand: Abcam Limited
SKU: AB154091

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(3 Publications)

Rabbit Polyclonal ACAA1/Beta-ketothiolase antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACAA1 aa 50 to C-terminus.

View Alternative Names

ACAA, PTHIO, ACAA1, Acetyl-CoA C-myristoyltransferase, Acetyl-CoA acyltransferase, Beta-ketothiolase, Peroxisomal 3-oxoacyl-CoA thiolase

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody (AB154091)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody (AB154091)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling ACAA1/Beta-ketothiolase with ab154091 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody (AB154091)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse stomach staining ACAA1/Beta-ketothiolase with ab154091 at a dilution of 1/500.

EDTA based Antigen Retrieval, pH 8.0 for 15min.

Unknown

Western blot - Anti-ACAA1/Beta-ketothiolase antibody (AB154091)

10% SDS PAGE

All lanes:

Western blot - Anti-ACAA1/Beta-ketothiolase antibody (ab154091) at 1/1000 dilution

Lane 1:

A549 whole cell lysate at 30 µg

Lane 2:

HeLa whole cell lysate at 30 µg

Predicted band size: 44 kDa

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Unknown

Western blot - Anti-ACAA1/Beta-ketothiolase antibody (AB154091)

10% SDS PAGE

All lanes:

Western blot - Anti-ACAA1/Beta-ketothiolase antibody (ab154091) at 1/1000 dilution

All lanes:

Mouse liver whole cell lysate at 50 µg

Predicted band size: 44 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ACAA1 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P09110

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACAA1 also known as Beta-ketothiolase is an enzyme involved in key metabolic processes. This enzyme with a molecular mass of approximately 41 kDa plays a pivotal role in the thiolytic cleavage of 3-ketoacyl-CoA an essential step in both fatty acid beta-oxidation and ketogenesis. ACAA1 is expressed in various tissues notably liver and muscle where high rates of fatty acid metabolism occur. As part of the peroxisome it functions effectively to maintain cellular lipid homeostasis.

Biological function summary

ACAA1 facilitates the metabolic breakdown of fatty acids by participating in the final steps of beta-oxidation. It does not form part of a larger enzymatic complex but it operates alongside enzymes like acyl-CoA oxidase and enoyl-CoA hydratase that contribute to the fatty acid oxidation pathway. Through its enzymatic activity ACAA1 directly influences energy production and metabolic rate which are important for maintaining cellular energy balance.

Pathways

Beta-ketothiolase plays an integral role in the fatty acid beta-oxidation and ketogenesis pathways. These pathways are critical for the breakdown of fatty acids into acetyl-CoA which enters the citric acid cycle for energy production. ACAA1 works closely with enzymes such as acetoacetyl-CoA thiolase and HMG-CoA lyase in these processes highlighting its involvement in converting fatty acids into usable energy forms.

Malfunction or deficiency in ACAA1 can lead to issues like ketoacidosis and metabolic dysregulation disorders. The enzyme's dysfunction can result in improper fatty acid metabolism and accumulation of toxic intermediates. ACAA1 shares a connection with HMG-CoA synthase in ketogenesis and any disruption in these pathways may contribute to metabolic diseases impacting energy homeostasis and leading to serious health conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Responsible for the thiolytic cleavage of straight chain 3-keto fatty acyl-CoAs (3-oxoacyl-CoAs) (PubMed : 11734571, PubMed : 2882519). Plays an important role in fatty acid peroxisomal beta-oxidation (PubMed : 11734571, PubMed : 2882519). Catalyzes the cleavage of short, medium, long, and very long straight chain 3-oxoacyl-CoAs (PubMed : 11734571, PubMed : 2882519).

See full target information ACAA1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Metabolites 15: PubMed40422873

2025

Compound 3d Attenuates Metabolic Dysfunction-Associated Steatohepatitis via Peroxisome Proliferator-Activated Receptor Pathway Activation and Inhibition of Inflammatory and Apoptotic Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shouqing Zhang,Jiajia Yu,Sule Bai,Shuhan Li,Quanyuan Qiu,Xiangshun Kong,Cen Xiang,Zhen Liu,Peng Yu,Yuou Teng

Allergologia et immunopathologia 53:160-168 PubMed40088033

2025

DUOX1 inhibits the progression of rheumatoid arthritis by regulating the NF-κB pathway in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Xuan,Dandan Feng,Fuyong Qiang,Yonghui Xia

Oxidative medicine and cellular longevity 2020:6821247 PubMed32855765

2020

Mitochondrial and Peroxisomal Alterations Contribute to Energy Dysmetabolism in Riboflavin Transporter Deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Fiorella Colasuonno,Alessia Niceforo,Chiara Marioli,Anna Fracassi,Fabrizia Stregapede,Keith Massey,Marco Tartaglia,Enrico Bertini,Claudia Compagnucci,Sandra Moreno

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ACAA1/Beta-ketothiolase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody (AB154091)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody (AB154091)

Western blot - Anti-ACAA1/Beta-ketothiolase antibody (AB154091)

Western blot - Anti-ACAA1/Beta-ketothiolase antibody (AB154091)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise