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AB210961

Anti-ACAA1/Beta-ketothiolase antibody [CL2650]

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Mouse Monoclonal ACAA1/Beta-ketothiolase antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ACAA1 aa 50-200.

View Alternative Names

ACAA, PTHIO, ACAA1, Acetyl-CoA C-myristoyltransferase, Acetyl-CoA acyltransferase, Beta-ketothiolase, Peroxisomal 3-oxoacyl-CoA thiolase

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (AB210961)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (AB210961)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human liver tissue labeling ACAA1/Beta-ketothiolase with ab210961 at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (AB210961)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (AB210961)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human prostate tissue labeling ACAA1/Beta-ketothiolase with ab210961 at 1/200 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (AB210961)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (AB210961)

PFA-fixed, Triton X-100 permeabilized CACO-2 cells stained for ACAA1 (Green) using ab210961 at 1 μg/ml. Microtubules are stained red, nuclei are stained blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (AB210961)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (AB210961)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human kidney tissue labeling ACAA1/Beta-ketothiolase with ab210961 at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (AB210961)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (AB210961)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human colon tissue labeling ACAA1/Beta-ketothiolase with ab210961 at 1/200 dilution.

Western blot - Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (AB210961)
  • WB

Supplier Data

Western blot - Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (AB210961)

All lanes:

Western blot - Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (ab210961) at 1/500 dilution

All lanes:

U251 cells

Predicted band size: 44 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2650

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ACAA1 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

P09110

Epitope

Binds to an epitope located within the peptide sequence GAIMARIAQFLSDIP as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACAA1 also known as Beta-ketothiolase is an enzyme involved in key metabolic processes. This enzyme with a molecular mass of approximately 41 kDa plays a pivotal role in the thiolytic cleavage of 3-ketoacyl-CoA an essential step in both fatty acid beta-oxidation and ketogenesis. ACAA1 is expressed in various tissues notably liver and muscle where high rates of fatty acid metabolism occur. As part of the peroxisome it functions effectively to maintain cellular lipid homeostasis.
Biological function summary

ACAA1 facilitates the metabolic breakdown of fatty acids by participating in the final steps of beta-oxidation. It does not form part of a larger enzymatic complex but it operates alongside enzymes like acyl-CoA oxidase and enoyl-CoA hydratase that contribute to the fatty acid oxidation pathway. Through its enzymatic activity ACAA1 directly influences energy production and metabolic rate which are important for maintaining cellular energy balance.

Pathways

Beta-ketothiolase plays an integral role in the fatty acid beta-oxidation and ketogenesis pathways. These pathways are critical for the breakdown of fatty acids into acetyl-CoA which enters the citric acid cycle for energy production. ACAA1 works closely with enzymes such as acetoacetyl-CoA thiolase and HMG-CoA lyase in these processes highlighting its involvement in converting fatty acids into usable energy forms.

Malfunction or deficiency in ACAA1 can lead to issues like ketoacidosis and metabolic dysregulation disorders. The enzyme's dysfunction can result in improper fatty acid metabolism and accumulation of toxic intermediates. ACAA1 shares a connection with HMG-CoA synthase in ketogenesis and any disruption in these pathways may contribute to metabolic diseases impacting energy homeostasis and leading to serious health conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Responsible for the thiolytic cleavage of straight chain 3-keto fatty acyl-CoAs (3-oxoacyl-CoAs) (PubMed : 11734571, PubMed : 2882519). Plays an important role in fatty acid peroxisomal beta-oxidation (PubMed : 11734571, PubMed : 2882519). Catalyzes the cleavage of short, medium, long, and very long straight chain 3-oxoacyl-CoAs (PubMed : 11734571, PubMed : 2882519).
See full target information ACAA1
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