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AB128911

Anti-ACAA2 antibody [EPR6733]

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(5 Publications)

Rabbit Recombinant Monoclonal ACAA2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Acetyl-CoA acetyltransferase, Acetyl-CoA acyltransferase, Beta-ketothiolase, Mitochondrial 3-oxoacyl-CoA thiolase, T1, ACAA2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-ACAA2 antibody [EPR6733] (AB128911)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ACAA2 antibody [EPR6733] (AB128911)

ab128911 stained HepG2 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab128911 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1hour at room temperature.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA2 antibody [EPR6733] (AB128911)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA2 antibody [EPR6733] (AB128911)

ab128911 at 1/100 dilution staining ACAA2 in paraffin embedded Human colonic carcinoma tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-ACAA2 antibody [EPR6733] (AB128911)
  • WB

Unknown

Western blot - Anti-ACAA2 antibody [EPR6733] (AB128911)

All lanes:

Western blot - Anti-ACAA2 antibody [EPR6733] (ab128911) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 41 kDa

false

OI-RD Scanning - Anti-ACAA2 antibody [EPR6733] (AB128911)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ACAA2 antibody [EPR6733] (AB128911)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-ACAA2 antibody [EPR6733] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6733

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACAA2 also known as acetyl-CoA acyltransferase 2 or 3-ketoacyl-CoA thiolase plays an important role in the mechanical process of fatty acid oxidation. It functions in the mitochondria where it catalyzes the final step of the beta-oxidation pathway facilitating the conversion of acetoacetyl-CoA to two molecules of acetyl-CoA. This protein has a known mass of approximately 43 kDa. ACAA2 is expressed in tissues with high energy demands such as the liver heart and muscle highlighting its significance in energy metabolism.
Biological function summary

The enzyme catalyzes reactions in energy metabolism by breaking down fatty acids to generate energy. ACAA2 functions as part of the complex mitochondrial fatty acid beta-oxidation machinery. This process supports energy production particularly under conditions of carbohydrate depletion. The production of acetyl-CoA by ACAA2 is important for the TCA cycle through which cells generate ATP the primary energy carrier.

Pathways

Acetyl-CoA acyltransferase 2 plays significant roles in both the fatty acid beta-oxidation and ketogenesis pathways. In the beta-oxidation pathway it works together with other enzymes like CPT1A and HADHB to efficiently break down fatty acids. In ketogenesis acetyl-CoA units produced by ACAA2 are directed towards the formation of ketone bodies which serve as an alternative energy source for the brain and muscles during fasting or prolonged exercise.

ACAA2 has connections to metabolic disorders particularly fatty acid oxidation disorders and diabetes. Deficiency in ACAA2 activity can result in accumulation and impaired degradation of fatty acids leading to metabolic imbalances. This enzyme also interacts with related proteins such as ACADM in the context of these metabolic diseases. Disruptions in ACAA2 function can face links to mitochondrial dysfunctions that contribute to diabetes due to its role in energy production and regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (Probable). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (Probable). Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies (Probable). Also displays hydrolase activity on various fatty acyl-CoAs (PubMed : 25478839). Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation (Probable). Abolishes BNIP3-mediated apoptosis and mitochondrial damage (PubMed : 18371312).
See full target information 3-ketoacyl-CoA thiolase, mitochondrial
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Biotechnology and applied biochemistry 72:104-115 PubMed39113388

2024

HPV18 E6/E7 activates Ca influx to promote the malignant progression of cervical cancer by inhibiting Ca binding protein 1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Kang,Lei Qiu,Yali Duo,FengLing Bi,Zhongjie Liu,Jing Wang,Lei Zheng,Ning Zhao

Frontiers in oncology 11:737127 PubMed34976793

2021

Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghui Lu,Yankun Zhang,Min Sun,Changyuan Ding,Lei Zhang,Youzi Kong,Meng Cai,Paolo Miccoli,Chunhong Ma,Xuetian Yue

Scientific reports 11:11816 PubMed34083649

2021

A mouse monoclonal antibody against influenza C virus attenuates acetaminophen-induced liver injury in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yutaka Sasaki,Naoto Yoshino,Takako Okuwa,Takashi Odagiri,Takashi Satoh,Yasushi Muraki

Chinese medicine 15:21 PubMed32158496

2020

Qishen granules exerts cardioprotective effects on rats with heart failure via regulating fatty acid and glucose metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Kuo Gao,Jian Zhang,Pengrong Gao,Qiyan Wang,Ying Liu,Junjie Liu,Yili Zhang,Yan Li,Hong Chang,Ping Ren,Jinmin Liu,Yong Wang,Wei Wang

Human molecular genetics 28:4132-4147 PubMed31646342

2019

The consequences of increased 4E-BP1 in polycystic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sara J Holditch,Carolyn N Brown,Daniel J Atwood,Deepak Pokhrel,Sara E Brown,Andrew M Lombardi,Khoa N Nguyen,Ryan C Hill,Miguel Lanaspa,Katharina Hopp,Mary C M Weiser-Evans,Charles L Edelstein
View all publications
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Product promise

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