AB128566

Anti-ACADL/LCAD antibody [7F5DD6]

Name: Anti-ACADL/LCAD antibody [7F5DD6] (ab128566) | Abcam
Brand: Abcam Limited
SKU: AB128566
Price: 405 USD
Availability: InStock

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(6 Publications)

Mouse Monoclonal ACADL/LCAD antibody. Suitable for IP, WB and reacts with Rat, Mouse samples. Cited in 6 publications.

View Alternative Names

LCAD, Acadl

2 Images

Unknown

Western blot - Anti-ACADL/LCAD antibody [7F5DD6] (AB128566)

All lanes:

Western blot - Anti-ACADL/LCAD antibody [7F5DD6] (ab128566) at 1 µg/mL

Lane 1:

Ladder

Lane 2:

Rat Liver Homogenate at 15 µg

Lane 3:

Rat Liver Mitochondria at 15 µg

Lane 4:

Rat H4IIE cell lysate at 15 µg

Lane 5:

Mouse Liver Homogenate at 15 µg

Lane 6:

Mouse Liver Mitochondria at 15 µg

Lane 7:

Mouse MEF cell lysate at 15 µg

Predicted band size: 47 kDa

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Immunoprecipitation - Anti-ACADL/LCAD antibody [7F5DD6] (AB128566)

Immunoprecipitation using ab128566

Lane 1 : Ladder
Lane 2 : Rat Liver Homogenate.

All lanes:

Immunoprecipitation - Anti-ACADL/LCAD antibody [7F5DD6] (ab128566)

Predicted band size: 47 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7F5DD6

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Mouse, Rat

Applications

IP, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Precipitation Ammonium Sulphate

Purification notes

Purity is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 99% HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACADL also known as LCAD (Long-Chain Acyl-CoA Dehydrogenase) is an enzyme important for fatty acid metabolism. It has a molecular mass of approximately 43 kDa. This protein is mainly found in mitochondria and you can find high expression levels in tissues with significant energy demands like liver muscle and heart. The mechanical function involves catalyzing the initial step in the beta-oxidation cycle which is the oxidation of long-chain acyl-CoA substrates to form a trans-double bond between the beta and alpha carbons.

Biological function summary

ACADL plays an essential role in mitochondrial energy production. It is part of the electron transport chain more specifically involved in the oxidation of fatty acids. This process is central to sustaining energy and heat production especially during fasting. ACADL works as part of a multi-enzyme complex known as the mitochondrial trifunctional protein complex responsible for multiple steps in fatty acid oxidation.

Pathways

ACADL's function in beta-oxidation links it to key metabolic pathways like energy metabolism and fatty acid catabolism. It directly interacts and works with other proteins such as enoyl-CoA hydratase and 3-hydroxyacyl-CoA dehydrogenase which facilitate subsequent steps after ACADL's initial action in the same beta-oxidation pathway. This integration ensures the proper breakdown of fatty acids to acetyl-CoA feeding into the citric acid cycle for ATP production.

Defects in ACADL activity are implicate in metabolic conditions such as long-chain acyl-CoA dehydrogenase deficiency (LCADD) and related cardiomyopathies. LCADD can lead to severe symptoms involving muscle weakness cardiomyopathy and hypoglycemia. ACADL's role in these disorders often intersects with other proteins like VLCAD (Very Long-Chain Acyl-CoA Dehydrogenase) since deficiencies or dysfunctions can manifest in overlapping symptoms due to their shared role in fatty acid oxidation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (By similarity). Among the different mitochondrial acyl-CoA dehydrogenases, long-chain specific acyl-CoA dehydrogenase can act on saturated and unsaturated acyl-CoAs with 6 to 24 carbons with a preference for 8 to 18 carbons long primary chains (PubMed : 9861014).

See full target information Acadl

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101714 PubMed39241774

2024

Detecting altered hepatic lipid oxidation by MRI in an animal model of MASLD.

Applications

Unspecified application

Species

Unspecified reactive species

Marc McLeod,Mario C Chang,Anna Rushin,Mukundan Ragavan,Rohit Mahar,Gaurav Sharma,Arshee Badar,Anthony Giacalone,Max E Glanz,Vinay R Malut,Dalton Graham,Nishanth E Sunny,James A Bankson,Kenneth Cusi,Matthew E Merritt

Scientific reports 14:1193 PubMed38216627

2024

Protein lysine acetylation does not contribute to the high rates of fatty acid oxidation seen in the post-ischemic heart.

Applications

Unspecified application

Species

Unspecified reactive species

Ezra B Ketema,Muhammad Ahsan,Liyan Zhang,Qutuba G Karwi,Gary D Lopaschuk

Lipids in health and disease 22:78 PubMed37344835

2023

Combining albumin deficiency and acute exercise reduces hepatic lipid droplet size in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhang,Mirandia Szramowski,Shuhan Sun,Gregory C Henderson

Cell death & disease 12:824 PubMed34471096

2021

Cyclic AMP-binding protein Epac1 acts as a metabolic sensor to promote cardiomyocyte lipotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Marion Laudette,Yannis Sainte-Marie,Grégoire Cousin,Dorian Bergonnier,Ismahane Belhabib,Stéphanie Brun,Karina Formoso,Loubna Laib,Florence Tortosa,Camille Bergoglio,Bertrand Marcheix,Jan Borén,Olivier Lairez,Jérémy Fauconnier,Alexandre Lucas,Jeanne Mialet-Perez,Cédric Moro,Frank Lezoualc'h

Cell reports 24:2919-2931 PubMed30208317

2018

Sarcolipin Signaling Promotes Mitochondrial Biogenesis and Oxidative Metabolism in Skeletal Muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Santosh K Maurya,Jose L Herrera,Sanjaya K Sahoo,Felipe C G Reis,Rick B Vega,Daniel P Kelly,Muthu Periasamy

Journal of molecular and cellular cardiology 112:64-73 PubMed28882480

2017

Short-term administration of Nicotinamide Mononucleotide preserves cardiac mitochondrial homeostasis and prevents heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Rongli Zhang,Yuyan Shen,Lin Zhou,Panjamaporn Sangwung,Hisashi Fujioka,Lilei Zhang,Xudong Liao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACADL/LCAD antibody [7F5DD6]

Western blot - Anti-ACADL/LCAD antibody [7F5DD6] (AB128566)

Immunoprecipitation - Anti-ACADL/LCAD antibody [7F5DD6] (AB128566)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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