AB152115

Anti-ACADM/MCAD antibody

Name: Anti-ACADM/MCAD antibody (ab152115) | Abcam
Brand: Abcam Limited
SKU: AB152115
Price: 545 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal ACADM/MCAD antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ACADM aa 1-100.

View Alternative Names

MCAD, Medium chain acyl-CoA dehydrogenase, MCADH, ACADM

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM/MCAD antibody (AB152115)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM/MCAD antibody (AB152115)

Immunohistochemical analysis of paraffin-embedded Human DLD1 xenograft tissue labeling ACADM/MCAD with ab152115 at 1/100 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-ACADM/MCAD antibody (AB152115)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ACADM/MCAD antibody (AB152115)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling ACADM/MCAD with ab152115 at 1/100 dilution. Lower image shows cells co-stained with Hoechst 33342.

Unknown

Western blot - Anti-ACADM/MCAD antibody (AB152115)

10% SDS PAGE

All lanes:

Western blot - Anti-ACADM/MCAD antibody (ab152115) at 1/1000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 41 kDa,47 kDa,62 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ACADM aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P11310

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACADM also known as MCAD (medium-chain acyl-CoA dehydrogenase) is an enzyme that plays a significant role in fatty acid metabolism. It weighs approximately 44 kDa and expresses mainly in the liver heart and skeletal muscles. Functions of MCAD involve catalyzing the initial step in the mitochondrial fatty acid β-oxidation spiral specifically for the medium-chain fatty acids. This enzyme helps in the conversion of fatty acyl-CoA to trans-enoyl-CoA through dehydrogenation.

Biological function summary

The enzyme facilitates energy production by breaking down medium-chain fatty acids within mitochondria. MCAD operates as a homotetramer complex where each subunit significantly contributes to its overall function. Such enzymatic activity is important for providing energy particularly when glycogen stores are low. Its efficient operation during fasting states indicates its importance in metabolic homeostasis.

Pathways

Medium-chain acyl-CoA dehydrogenase (MCAD) is integral to the mitochondrial fatty acid beta-oxidation pathway. This pathway is a primary route for fatty acid catabolism ultimately leading to energy production in the form of ATP. MCAD's activity also relates to other fatty acid oxidation enzymes like VLCAD (very-long-chain acyl-CoA dehydrogenase) and SCAD (short-chain acyl-CoA dehydrogenase) which operate on different chain-length fatty acids cooperating to maintain energy balance.

MCAD deficiency is a common metabolic disorder that impairs the normal breakdown of fatty acids. This condition results in the accumulation of fatty acid intermediates leading to hypoketotic hypoglycemia during fasting periods. Individuals with MCAD deficiency may experience lethargy vomiting and seizures. The disorder connects with other enzymes such as LCHAD (long-chain 3-hydroxyacyl-CoA dehydrogenase) involved in similar pathways and deficiencies can result in related metabolic dysfunctions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation (FAO), breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (PubMed : 1970566, PubMed : 21237683, PubMed : 2251268, PubMed : 8823175). The first step of FAO consists in the proR-proR stereospecific alpha, beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA) (PubMed : 2251268). ETF is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (PubMed : 15159392, PubMed : 25416781). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase has preference for fatty acyl-CoAs with saturated 6 to 12 carbons long primary chains, making it but can also catalyze longer chains such as C14 and C16 (PubMed : 1970566, PubMed : 21237683, PubMed : 2251268, PubMed : 8823175).

See full target information ACADM

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 46:1135-1145 PubMed32705160

2020

Propofol‑induced HOXA11‑AS promotes proliferation, migration and invasion, but inhibits apoptosis in hepatocellular carcinoma cells by targeting miR‑4458.

Applications

Unspecified application

Species

Unspecified reactive species

Furong Song,Jun Liu,Yawei Feng,Yi Jin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACADM/MCAD antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM/MCAD antibody (AB152115)

Immunocytochemistry/ Immunofluorescence - Anti-ACADM/MCAD antibody (AB152115)

Western blot - Anti-ACADM/MCAD antibody (AB152115)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise