Skip to main content

Rabbit Recombinant Monoclonal ACADM/MCAD antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.


Images

Immunoprecipitation - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (AB239914), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (AB239914), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (AB239914), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (AB239914), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (AB239914), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Expected
Expected
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Expected
Rat
Predicted
Predicted
Expected
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

2 products for Alternative Version

Target data

Function

Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (PubMed:1970566, PubMed:21237683, PubMed:2251268, PubMed:8823175). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (PubMed:2251268). Electron transfer flavoprotein (ETF) is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (PubMed:15159392, PubMed:25416781). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 6 to 12 carbons long primary chains (PubMed:1970566, PubMed:21237683, PubMed:2251268, PubMed:8823175).

Alternative names

MCAD, Medium chain acyl-CoA dehydrogenase, MCADH, ACADM

Recommended products

Rabbit Recombinant Monoclonal ACADM/MCAD antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR3708
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab239914 is the carrier-free version of Anti-ACADM/MCAD antibody [EPR3708] ab92461.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ACADM also known as MCAD (medium-chain acyl-CoA dehydrogenase) is an enzyme that plays a significant role in fatty acid metabolism. It weighs approximately 44 kDa and expresses mainly in the liver heart and skeletal muscles. Functions of MCAD involve catalyzing the initial step in the mitochondrial fatty acid β-oxidation spiral specifically for the medium-chain fatty acids. This enzyme helps in the conversion of fatty acyl-CoA to trans-enoyl-CoA through dehydrogenation.

Biological function summary

The enzyme facilitates energy production by breaking down medium-chain fatty acids within mitochondria. MCAD operates as a homotetramer complex where each subunit significantly contributes to its overall function. Such enzymatic activity is important for providing energy particularly when glycogen stores are low. Its efficient operation during fasting states indicates its importance in metabolic homeostasis.

Pathways

Medium-chain acyl-CoA dehydrogenase (MCAD) is integral to the mitochondrial fatty acid beta-oxidation pathway. This pathway is a primary route for fatty acid catabolism ultimately leading to energy production in the form of ATP. MCAD's activity also relates to other fatty acid oxidation enzymes like VLCAD (very-long-chain acyl-CoA dehydrogenase) and SCAD (short-chain acyl-CoA dehydrogenase) which operate on different chain-length fatty acids cooperating to maintain energy balance.

Associated diseases and disorders

MCAD deficiency is a common metabolic disorder that impairs the normal breakdown of fatty acids. This condition results in the accumulation of fatty acid intermediates leading to hypoketotic hypoglycemia during fasting periods. Individuals with MCAD deficiency may experience lethargy vomiting and seizures. The disorder connects with other enzymes such as LCHAD (long-chain 3-hydroxyacyl-CoA dehydrogenase) involved in similar pathways and deficiencies can result in related metabolic dysfunctions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunoprecipitation - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (ab239914), expandable thumbnail

    Immunoprecipitation - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (ab239914)

    Anti-ACADM/MCAD antibody [EPR3708] ab92461 (purified) at 1/30 dilution (2 µg) immunoprecipitating ACADM/MCAD in Mouse heart lysate.
    Lane 1 (input): Mouse heart lysate 10 µg
    Lane 2 (+): Anti-ACADM/MCAD antibody [EPR3708] ab92461 & Mouse heart lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ACADM/MCAD antibody [EPR3708] ab92461 in Mouse heart lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ACADM/MCAD antibody [EPR3708] ab92461)

    All lanes: Immunoprecipitation - Anti-ACADM/MCAD antibody [EPR3708] (Anti-ACADM/MCAD antibody [EPR3708] ab92461)

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (ab239914), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (ab239914)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling ACADM/MCAD with purified Anti-ACADM/MCAD antibody [EPR3708] ab92461 at 1/600 dilution (1.02 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ACADM/MCAD antibody [EPR3708] ab92461)

  • Immunocytochemistry/ Immunofluorescence - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (ab239914), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (ab239914)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ACADM/MCAD with purified Anti-ACADM/MCAD antibody [EPR3708] ab92461 at 1:50 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (anti acadm mcad antibody epr3708 immunocytochemistry hela human)

  • Flow Cytometry (Intracellular) - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (ab239914), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (ab239914)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ACADM/MCAD with purified Anti-ACADM/MCAD antibody [EPR3708] ab92461 at 1/60 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ACADM/MCAD antibody [EPR3708] ab92461)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (ab239914), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (ab239914)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling ACADM/MCAD with purified Anti-ACADM/MCAD antibody [EPR3708] ab92461 at 1/600 dilution (1.02 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ACADM/MCAD antibody [EPR3708] ab92461)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (ab239914), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (ab239914)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling ACADM/MCAD with purified Anti-ACADM/MCAD antibody [EPR3708] ab92461 at 1/600 dilution (1.02 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ACADM/MCAD antibody [EPR3708] ab92461)

  • Flow Cytometry (Intracellular) - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (ab239914), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (ab239914)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ACADM/MCAD with unpurified Anti-ACADM/MCAD antibody [EPR3708] ab92461 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ACADM/MCAD antibody [EPR3708] ab92461).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (ab239914), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (ab239914)

    Anti-ACADM/MCAD antibody [EPR3708] ab92461 (unpurified), at 1/100 dilution, staining ACADM/MCAD in formalin-fixed, paraffin-embedded Human liver tissue by immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ACADM/MCAD antibody [EPR3708] ab92461).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (ab239914), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ACADM/MCAD antibody [EPR3708] - BSA and Azide free (ab239914)

    Immunofluorescent staining of ACADM/MCAD in HeLa cells using Anti-ACADM/MCAD antibody [EPR3708] ab92461 (unpurified) at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ACADM/MCAD antibody [EPR3708] ab92461).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com