AB154823

Anti-ACADS/SCAD antibody [EPR10861(B)]

RabMAb
Recombinant
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(10 Publications)

Rabbit Recombinant Monoclonal ACADS/SCAD antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 10 publications.

View Alternative Names

SCAD, Butyryl-CoA dehydrogenase, ACADS

2 Images

Flow Cytometry (Intracellular) - Anti-ACADS/SCAD antibody [EPR10861(B)] (AB154823)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ACADS/SCAD antibody [EPR10861(B)] (AB154823)

Intracellular flow cytometric analysis of permeabilized 293T cells labeling ACADS/SCAD using ab154823 (red) at 1/10 dilution, or rabbit IgG (negative) (green).

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Western blot - Anti-ACADS/SCAD antibody [EPR10861(B)] (AB154823)

All lanes:

Western blot - Anti-ACADS/SCAD antibody [EPR10861(B)] (ab154823) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

Human fetal heart tissue lysate at 10 µg

Lane 5:

Human fetal liver tissue lysate at 10 µg

Predicted band size: 44 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10861(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ACADS protein also known as short-chain acyl-CoA dehydrogenase (SCAD) is an enzyme that plays a mechanical role in the mitochondrial fatty acid beta-oxidation process. It catalyzes the dehydrogenation of acyl-CoA substrates specifically short-chain fatty acids. The molecular mass of the ACADS protein is approximately 44 kDa. Expression of the ACADS protein occurs in the mitochondria of various tissues especially in liver and muscle tissues where fatty acid metabolism is active.

Biological function summary

The function of ACADS involves the conversion of fatty acids into acetyl-CoA an essential step in energy production. ACADS operates in concert with other enzymes in the mitochondrial matrix contributing to the beta-oxidation cycle. This enzyme is essential for maintaining energy homeostasis particularly during periods of fasting or increased energy demands. Although ACADS is not a component of a larger enzyme complex it works closely with other dehydrogenases that metabolize different chain lengths of fatty acids.

Pathways

The ACADS enzyme is critically involved in the fatty acid beta-oxidation pathway. This pathway is vital for energy production especially in cardiac and skeletal muscles. ACADS is functionally related to other enzymes such as MCAD (medium-chain acyl-CoA dehydrogenase) and VLCAD (very long-chain acyl-CoA dehydrogenase) through the sequential processing of fatty acids of varying chain lengths. Proper functioning of the beta-oxidation pathway ensures that fatty acids are systematically broken down to meet the body’s energetic needs.

Defects in ACADS are associated with metabolic disorders namely short-chain acyl-CoA dehydrogenase deficiency (SCADD). This condition can result in muscle weakness developmental delay and hypoglycemia. ACADS is also indirectly linked to disorders involving MCAD as both enzymes function within the same metabolic pathway. Accurate diagnosis and management of SCADD rely on understanding the role of ACADS and its interactions with interconnected proteins in the metabolic network.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Short-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (By similarity). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (By similarity). Among the different mitochondrial acyl-CoA dehydrogenases, short-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 4 to 6 carbons long primary chains (PubMed : 11134486, PubMed : 21237683).

See full target information Short-chain specific acyl-CoA dehydrogenase, mitochondrial

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:110853 PubMed39310762

2024

Crosstalk between butyrate oxidation in colonocyte and butyrate-producing bacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Bohye Park,Ji Yeon Kim,Olivia F Riffey,Triston J Walsh,Jeremiah Johnson,Dallas R Donohoe

Experimental physiology 108:874-890 PubMed37184360

2023

Cardiomyocyte tetrahydrobiopterin synthesis regulates fatty acid metabolism and susceptibility to ischaemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Sandy M Chu,Lisa C Heather,Surawee Chuaiphichai,Thomas Nicol,Benjamin Wright,Matthieu Miossec,Jennifer K Bendall,Gillian Douglas,Mark J Crabtree,Keith M Channon

Scientific reports 12:8771 PubMed35610475

2022

Pyruvate kinase M1 regulates butyrate metabolism in cancerous colonocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Bohye Park,Ji Yeon Kim,Olivia F Riffey,Presley Dowker-Key,Antje Bruckbauer,James McLoughlin,Ahmed Bettaieb,Dallas R Donohoe

Poultry science 100:101342 PubMed34438327

2021

Effects of riboflavin deficiency on the lipid metabolism of duck breeders and duck embryos.

Applications

Unspecified application

Species

Unspecified reactive species

B Zhang,J Tang,Y B Wu,J T Cao,G N Xing,P X Sun,W Huang,M Xie,S S Hou

International journal of molecular sciences 21: PubMed32098258

2020

The Influence of Statins on the Aerobic Metabolism of Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Izabela Broniarek,Karolina Dominiak,Lukasz Galganski,Wieslawa Jarmuszkiewicz

Molecular cell 69:729-743.e7 PubMed29499131

2018

Dynamic Regulation of Long-Chain Fatty Acid Oxidation by a Noncanonical Interaction between the MCL-1 BH3 Helix and VLCAD.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Escudero,Elma Zaganjor,Susan Lee,Christopher P Mill,Ann M Morgan,Emily B Crawford,Jiahao Chen,Thomas E Wales,Rida Mourtada,James Luccarelli,Gregory H Bird,Ulrich Steidl,John R Engen,Marcia C Haigis,Joseph T Opferman,Loren D Walensky

The British journal of nutrition 118:641-650 PubMed29185933

2017

Severe riboflavin deficiency induces alterations in the hepatic proteome of starter Pekin ducks.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Tang,Maria A Hegeman,Jian Hu,Ming Xie,Wenbiao Shi,Yong Jiang,Vincent de Boer,Yuming Guo,Shuisheng Hou,Jaap Keijer

Pflugers Archiv : European journal of physiology 469:815-827 PubMed28176017

2017

Hypoxia and aerobic metabolism adaptations of human endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Agnieszka Koziel,Wieslawa Jarmuszkiewicz

Pflugers Archiv : European journal of physiology 468:1541-54 PubMed27417103

2016

The effect of chronic exposure to high palmitic acid concentrations on the aerobic metabolism of human endothelial EA.hy926 cells.

Applications

Species

Human

Izabela Broniarek,Agnieszka Koziel,Wieslawa Jarmuszkiewicz

Molecular & cellular proteomics : MCP 15:1728-39 PubMed26850065

2016

Ataxin-2 (Atxn2)-Knock-Out Mice Show Branched Chain Amino Acids and Fatty Acids Pathway Alterations.

Applications

Unspecified application

Species

Unspecified reactive species

David Meierhofer,Melanie Halbach,Nesli Ece Şen,Suzana Gispert,Georg Auburger

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACADS/SCAD antibody [EPR10861(B)]

Flow Cytometry (Intracellular) - Anti-ACADS/SCAD antibody [EPR10861(B)] (AB154823)

Western blot - Anti-ACADS/SCAD antibody [EPR10861(B)] (AB154823)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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