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AB156571

Anti-ACADS/SCAD antibody [EPR10862(B)]

5

(2 Reviews)

|

(7 Publications)

Rabbit Recombinant Monoclonal ACADS/SCAD antibody. Suitable for IHC-P, IP, WB and reacts with Human, Rat, Mouse samples. Cited in 7 publications.

View Alternative Names

SCAD, Butyryl-CoA dehydrogenase, ACADS

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)

ab156571 showing +ve staining in Human gastric adenocarcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)

ab156571 showing +ve staining in Human urinary bladder carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)

ab156571 showing +ve staining in Human normal liver.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)

Immunohistochemical analysis of paraffin embedded Human thyroid gland tissue labeling ACADS/SCAD with ab156571 antibody at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)

ab156571 showing +ve staining in Human normal colon.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue sections labeling ACADS/SCAD with Purified ab156571 at 1 : 100 dilution (3.76 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)

Immunohistochemical analysis of paraffin embedded Human heart tissue labeling ACADS/SCAD with ab156571 antibody at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)
  • IP

Unknown

Immunoprecipitation - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)

ab156571 (Purified) at 1 : 20 dilution (2 µg) immunoprecipitating ACADS/SCAD in Human liver lysate.
Lane 1 (input) : Human liver lysate 10 µg
Lane 2 (+) : ab156571 & Human liver lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab156571 in Human liver lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156571)

All lanes:

Immunoprecipitation - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)

Predicted band size: 44 kDa

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Western blot - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)
  • WB

Lab

Western blot - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)

All lanes:

Western blot - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

Mouse liver lysates at 20 µg

Lane 3:

Rat kidney lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 44 kDa

Observed band size: 41 kDa

false

  • Carrier free

    Anti-ACADS/SCAD antibody [EPR10862(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10862(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p>The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.</p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ACADS protein also known as short-chain acyl-CoA dehydrogenase (SCAD) is an enzyme that plays a mechanical role in the mitochondrial fatty acid beta-oxidation process. It catalyzes the dehydrogenation of acyl-CoA substrates specifically short-chain fatty acids. The molecular mass of the ACADS protein is approximately 44 kDa. Expression of the ACADS protein occurs in the mitochondria of various tissues especially in liver and muscle tissues where fatty acid metabolism is active.
Biological function summary

The function of ACADS involves the conversion of fatty acids into acetyl-CoA an essential step in energy production. ACADS operates in concert with other enzymes in the mitochondrial matrix contributing to the beta-oxidation cycle. This enzyme is essential for maintaining energy homeostasis particularly during periods of fasting or increased energy demands. Although ACADS is not a component of a larger enzyme complex it works closely with other dehydrogenases that metabolize different chain lengths of fatty acids.

Pathways

The ACADS enzyme is critically involved in the fatty acid beta-oxidation pathway. This pathway is vital for energy production especially in cardiac and skeletal muscles. ACADS is functionally related to other enzymes such as MCAD (medium-chain acyl-CoA dehydrogenase) and VLCAD (very long-chain acyl-CoA dehydrogenase) through the sequential processing of fatty acids of varying chain lengths. Proper functioning of the beta-oxidation pathway ensures that fatty acids are systematically broken down to meet the body’s energetic needs.

Defects in ACADS are associated with metabolic disorders namely short-chain acyl-CoA dehydrogenase deficiency (SCADD). This condition can result in muscle weakness developmental delay and hypoglycemia. ACADS is also indirectly linked to disorders involving MCAD as both enzymes function within the same metabolic pathway. Accurate diagnosis and management of SCADD rely on understanding the role of ACADS and its interactions with interconnected proteins in the metabolic network.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Short-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (By similarity). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (By similarity). Among the different mitochondrial acyl-CoA dehydrogenases, short-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 4 to 6 carbons long primary chains (PubMed : 11134486, PubMed : 21237683).
See full target information Short-chain specific acyl-CoA dehydrogenase, mitochondrial
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Animal cells and systems 29:512-522 PubMed40800577

2025

RNA binding protein HuD regulates fatty acid oxidation in pancreatic β-cells by modulating long-chain acyl-CoA dehydrogenase expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyoon Seo,Seungyeon Ryu,Wei Zhang,Eun Kyung Lee,Seung Min Jeong

Aging cell 23:e14256 PubMed38898632

2024

Aging-induced short-chain acyl-CoA dehydrogenase promotes age-related hepatic steatosis by suppressing lipophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Deng,Shanshan Yang,Xiaoqian Yu,Ruixue Zhou,Yin Liu,Hongmei Zhang,Daxin Cui,Xingrong Feng,Yanting Wu,Xiaocun Qi,Zhiguang Su

Frontiers in molecular neuroscience 15:1064509 PubMed36743291

2023

Folate-deficiency induced acyl-CoA synthetase short-chain family member 2 increases lysine crotonylome involved in neural tube defects.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Wang,Yubing Zeng,Xuejia He,Fan Liu,Pei Pei,Ting Zhang

Cellular and molecular life sciences : CMLS 79:375 PubMed35727412

2022

Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Zhi Peng,Yong-Xian Shao,Xiu-Zhen Li,Kang-Di Zhang,Yan-Na Cai,Yun-Ting Lin,Min-Yan Jiang,Zong-Cai Liu,Xue-Ying Su,Wen Zhang,Xiao-Ling Jiang,Li Liu

Journal of cancer prevention 26:32-40 PubMed33842404

2021

Dysregulated Free Fatty Acid Receptor 2 Exacerbates Colonic Adenoma Formation in Mice: Relation to Metabolism and Gut Microbiota Composition.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Wen Huang,Chien-Wei Lin,Pan Pan,Carla Elena Echeveste,Athena Dong,Kiyoko Oshima,Martha Yearsley,Jianhua Yu,Li-Shu Wang

Biochemical pharmacology 178:114100 PubMed32540485

2020

Flavine adenine dinucleotide inhibits pathological cardiac hypertrophy and fibrosis through activating short chain acyl-CoA dehydrogenase.

Applications

Unspecified application

Species

Unspecified reactive species

Zhichao Ma,Xue Qin,Xiaoyi Zhong,Yingqing Liao,Yongshao Su,Xi Liu,Peiqing Liu,Jing Lu,Sigui Zhou

Journal of cellular and molecular medicine 20:1381-91 PubMed26989860

2016

Effects of short-chain acyl-CoA dehydrogenase on cardiomyocyte apoptosis.

Applications

WB

Species

Rat

Zhenhua Zeng,Qiuju Huang,Zhaohui Shu,Peiqing Liu,Shaorui Chen,Xuediao Pan,Linquan Zang,Sigui Zhou
View all publications
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