AB110285

Anti-ACADVL/VLCAD antibody [6A9AF2]

Name: Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285) | Abcam
Brand: Abcam Limited
SKU: AB110285

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(1 Publication)

Mouse Monoclonal ACADVL/VLCAD antibody. Suitable for IP, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

VLCAD, ACADVL

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-ACADVL/VLCAD antibody [6A9AF2] (AB110285)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ACADVL/VLCAD antibody [6A9AF2] (AB110285)

Immunocytochemistry image of ACADVL/VLCAD antibody [6A9AF2] (ab110285) stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110285 at 5 μg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [6A9AF2] (AB110285)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [6A9AF2] (AB110285)

ab110285,at 1/250 dilution, staining ACADVL/VLCAD in formalin-fixed, paraffin-embedded human cerebellum tissue. Immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.

Unknown

Immunoprecipitation - Anti-ACADVL/VLCAD antibody [6A9AF2] (AB110285)

ACADVL/VLCAD immunocaptured from 0.75mg of human liver mitochondria lysate /10ul antibody conjugated beads using ab110285.

All lanes:

Immunoprecipitation - Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285)

Predicted band size: 70 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6A9AF2

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Proprietary technique

Purification notes

The antibody was produced in vitro using hybridoma grown in serum-free media and then purified by biochemical fractionation. Purity >95% by SDS-PAGE.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACADVL also known as VLCAD (Very Long-Chain Acyl-CoA Dehydrogenase) is an enzyme that plays an important role in the breakdown of long-chain fatty acids within the mitochondria. It has a molecular mass of approximately 70 kDa. VLCAD is expressed in various tissues with high levels found in the heart skeletal muscle and liver. These tissues are heavily reliant on fatty acid oxidation for energy production especially when glucose availability is low.

Biological function summary

ACADVL functions within the mitochondrial matrix where it catalyzes the initial step of beta-oxidation by desaturating long-chain acyl-CoA molecules. This enzyme is a part of a larger complex that includes other mitochondrial dehydrogenases contributing to the degradation of fatty acids into acetyl-CoA units. The process is essential for energy production especially under fasting conditions or high-energy demand scenarios such as muscle contraction and cardiac function.

Pathways

VLCAD plays an essential role in the fatty acid beta-oxidation pathway. It works alongside other enzymes such as ACADM (Medium-Chain Acyl-CoA Dehydrogenase) to facilitate the sequential shortening of fatty acids which integrates into the Krebs cycle for further energy extraction. This pathway maximizes energy yield from fatty acids significantly influencing cellular metabolism and energy homeostasis.

VLCAD deficiencies lead to severe metabolic conditions such as VLCAD deficiency. This disorder results in the impaired breakdown of long-chain fatty acids causing energy deficiency and the accumulation of toxic substances. Symptoms range from muscle weakness to serious cardiac complications. VLCAD deficiency's impact is often assessed in relation to proteins like ACSL1 and CPT2 which are involved in the fatty acid metabolism pathway and are central to effective energy processing in cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation (FAO), breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (PubMed : 17564966, PubMed : 18227065, PubMed : 7668252, PubMed : 9461620, PubMed : 9599005, PubMed : 9839948). The first step of FAO consists in the proR-proR stereospecific alpha, beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA) (PubMed : 18227065, PubMed : 7668252, PubMed : 9461620, PubMed : 9839948). Among the different mitochondrial acyl-CoA dehydrogenases, very long-chain specific acyl-CoA dehydrogenase acts specifically on fatty acyl-CoAs with saturated 12 to 24 carbons long primary chains (PubMed : 17564966, PubMed : 21237683, PubMed : 9839948).

See full target information ACADVL

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 6:e25249 PubMed21966468

2011

A toll-like receptor 2 pathway regulates the Ppargc1a/b metabolic co-activators in mice with Staphylococcal aureus sepsis.

Applications

Species

Mouse

Timothy E Sweeney,Hagir B Suliman,John W Hollingsworth,Karen E Welty-Wolf,Claude A Piantadosi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACADVL/VLCAD antibody [6A9AF2]

Immunocytochemistry/ Immunofluorescence - Anti-ACADVL/VLCAD antibody [6A9AF2] (AB110285)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [6A9AF2] (AB110285)

Immunoprecipitation - Anti-ACADVL/VLCAD antibody [6A9AF2] (AB110285)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise