Mouse Monoclonal ACADVL/VLCAD antibody. Suitable for IP, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-ACADVL/VLCAD antibody [6A9AF2] (AB110285), expandable thumbnail](https://content.abcam.com/products/images/acadvl-vlcad-antibody-6a9af2-ab110285--immunocytochemistry-immunofluorescence-img74758.gif "Immunocytochemistry/ Immunofluorescence - Anti-ACADVL/VLCAD antibody [6A9AF2] (AB110285)")
Publications
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- PloS one 6:e252492011A toll-like receptor 2 pathway regulates the Ppargc1a/b metabolic co-activators in mice with Staphylococcal aureus sepsis.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (PubMed:18227065, PubMed:7668252, PubMed:9461620, PubMed:9599005, PubMed:9839948). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (PubMed:18227065, PubMed:7668252, PubMed:9461620, PubMed:9839948). Among the different mitochondrial acyl-CoA dehydrogenases, very long-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 12 to 24 carbons long primary chains (PubMed:21237683, PubMed:9839948).
Alternative names
VLCAD, ACADVL, VLCAD, VLCAD
Recommended products
Mouse Monoclonal ACADVL/VLCAD antibody. Suitable for IP, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
6A9AF2
- Purification technique
Proprietary technique
- Light chain type
kappa
- Concentration
- Purification notes
The antibody was produced in vitro using hybridoma grown in serum-free media and then purified by biochemical fractionation. Purity >95% by SDS-PAGE.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product was previously marketed under the MitoSciences sub-brand.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ACADVL also known as VLCAD (Very Long-Chain Acyl-CoA Dehydrogenase) is an enzyme that plays an important role in the breakdown of long-chain fatty acids within the mitochondria. It has a molecular mass of approximately 70 kDa. VLCAD is expressed in various tissues with high levels found in the heart skeletal muscle and liver. These tissues are heavily reliant on fatty acid oxidation for energy production especially when glucose availability is low.
- Biological function summary
ACADVL functions within the mitochondrial matrix where it catalyzes the initial step of beta-oxidation by desaturating long-chain acyl-CoA molecules. This enzyme is a part of a larger complex that includes other mitochondrial dehydrogenases contributing to the degradation of fatty acids into acetyl-CoA units. The process is essential for energy production especially under fasting conditions or high-energy demand scenarios such as muscle contraction and cardiac function.
- Pathways
VLCAD plays an essential role in the fatty acid beta-oxidation pathway. It works alongside other enzymes such as ACADM (Medium-Chain Acyl-CoA Dehydrogenase) to facilitate the sequential shortening of fatty acids which integrates into the Krebs cycle for further energy extraction. This pathway maximizes energy yield from fatty acids significantly influencing cellular metabolism and energy homeostasis.
- Associated diseases and disorders
VLCAD deficiencies lead to severe metabolic conditions such as VLCAD deficiency. This disorder results in the impaired breakdown of long-chain fatty acids causing energy deficiency and the accumulation of toxic substances. Symptoms range from muscle weakness to serious cardiac complications. VLCAD deficiency's impact is often assessed in relation to proteins like ACSL1 and CPT2 which are involved in the fatty acid metabolism pathway and are central to effective energy processing in cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285)
Immunocytochemistry image of ACADVL/VLCAD antibody [6A9AF2] (ab110285) stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110285 at 5 μg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285), expandable thumbnail](https://content.abcam.com/products/images/acadvl-vlcad-antibody-6a9af2-ab110285--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101655.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285)
ab110285,at 1/250 dilution, staining ACADVL/VLCAD in formalin-fixed, paraffin-embedded human cerebellum tissue. Immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.
![Immunoprecipitation - Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285), expandable thumbnail](https://content.abcam.com/products/images/acadvl-vlcad-antibody-6a9af2-ab110285--immunoprecipitation-img3184.gif "Immunoprecipitation - Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285)")
Immunoprecipitation - Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285)
ACADVL/VLCAD immunocaptured from 0.75mg of human liver mitochondria lysate /10ul antibody conjugated beads using ab110285.
All lanes: Immunoprecipitation - Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285)
Predicted band size: 70 kDa
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