Rabbit Polyclonal ACADVL/VLCAD antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Very long-chain specific acyl-CoA dehydrogenase, mitochondrial aa 1-350.
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- Scientific reports 12:145762022PERM1 regulates genes involved in fatty acid metabolism in the heart by interacting with PPARα and PGC-1α.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Yang Huang,Shin-Ichi Oka,Xiaoyong Xu,Chian-Feng Chen,Chien-Yi Tung,Ya-Yuan Chang,Youssef Mourad,Omair Vehra,Andreas Ivessa,Ghassan Yehia,Peter Romanienko,Chiao-Po Hsu,Junichi SadoshimaPubMed 36028747
- Cellular and molecular life sciences : CMLS 79:3752022Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin-Zhi Peng,Yong-Xian Shao,Xiu-Zhen Li,Kang-Di Zhang,Yan-Na Cai,Yun-Ting Lin,Min-Yan Jiang,Zong-Cai Liu,Xue-Ying Su,Wen Zhang,Xiao-Ling Jiang,Li LiuPubMed 35727412
- Theranostics 10:8197-82102020Brown adipose tissue-derived exosomes mitigate the metabolic syndrome in high fat diet mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXueying Zhou et. al.PubMed 32724466
- Molecular metabolism 37:1009812020Mitochondrial disease disrupts hepatic allostasis and lowers the threshold for immune-mediated liver toxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaxim Jestin et. al.PubMed 32283081
- American journal of physiology. Endocrinology and metabolism 318:E145-E1512019Combined overexpression of SIRT1 and knockout of GCN5 in adult skeletal muscle does not affect glucose homeostasis or exercise performance in mice.Applications:WBReactive species:MouseKristoffer Svensson,Shahriar Tahvilian,Vitor F Martins,Jessica R Dent,Adrianna Lemanek,Neeka Barooni,Keenan Greyslak,Carrie E McCurdy,Simon SchenkPubMed 31794263
- Oxidative medicine and cellular longevity 2019:98942382019Kynurenine, a Tryptophan Metabolite That Increases with Age, Induces Muscle Atrophy and Lipid Peroxidation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHelen Kaiser et. al.PubMed 31737181
- Bioscience reports 39:2019Distinct metabolic patterns during microglial remodeling by oleate and palmitate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBruno Chausse et. al.PubMed 30867255
- PloS one 14:e02117332019Resilient hepatic mitochondrial function and lack of iNOS dependence in diet-induced insulin resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPamela A Kakimoto et. al.PubMed 30716103
- Acta physiologica (Oxford, England) 221:193-2032017Temporal overexpression of SIRT1 in skeletal muscle of adult mice does not improve insulin sensitivity or markers of mitochondrial biogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesK Svensson et. al.PubMed 28544355
- Oncotarget 7:75279-752922016High-throughput proteomics integrated with gene microarray for discovery of colorectal cancer potential biomarkers.Applications:IHC-PReactive species:HumanJiekai Yu,Xiaofen Li,Chenhan Zhong,Dan Li,Xiaohui Zhai,Wangxiong Hu,Cheng Guo,Ying Yuan,Shu ZhengPubMed 27661117
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Very long-chain specific acyl-CoA dehydrogenase, mitochondrial aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link P49748
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (PubMed:18227065, PubMed:7668252, PubMed:9461620, PubMed:9599005, PubMed:9839948). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (PubMed:18227065, PubMed:7668252, PubMed:9461620, PubMed:9839948). Among the different mitochondrial acyl-CoA dehydrogenases, very long-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 12 to 24 carbons long primary chains (PubMed:21237683, PubMed:9839948).
Alternative names
VLCAD, ACADVL, VLCAD, VLCAD
Recommended products
Rabbit Polyclonal ACADVL/VLCAD antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Very long-chain specific acyl-CoA dehydrogenase, mitochondrial aa 1-350.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Very long-chain specific acyl-CoA dehydrogenase, mitochondrial aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link P49748
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ACADVL also known as VLCAD (Very Long-Chain Acyl-CoA Dehydrogenase) is an enzyme that plays an important role in the breakdown of long-chain fatty acids within the mitochondria. It has a molecular mass of approximately 70 kDa. VLCAD is expressed in various tissues with high levels found in the heart skeletal muscle and liver. These tissues are heavily reliant on fatty acid oxidation for energy production especially when glucose availability is low.
- Biological function summary
ACADVL functions within the mitochondrial matrix where it catalyzes the initial step of beta-oxidation by desaturating long-chain acyl-CoA molecules. This enzyme is a part of a larger complex that includes other mitochondrial dehydrogenases contributing to the degradation of fatty acids into acetyl-CoA units. The process is essential for energy production especially under fasting conditions or high-energy demand scenarios such as muscle contraction and cardiac function.
- Pathways
VLCAD plays an essential role in the fatty acid beta-oxidation pathway. It works alongside other enzymes such as ACADM (Medium-Chain Acyl-CoA Dehydrogenase) to facilitate the sequential shortening of fatty acids which integrates into the Krebs cycle for further energy extraction. This pathway maximizes energy yield from fatty acids significantly influencing cellular metabolism and energy homeostasis.
- Associated diseases and disorders
VLCAD deficiencies lead to severe metabolic conditions such as VLCAD deficiency. This disorder results in the impaired breakdown of long-chain fatty acids causing energy deficiency and the accumulation of toxic substances. Symptoms range from muscle weakness to serious cardiac complications. VLCAD deficiency's impact is often assessed in relation to proteins like ACSL1 and CPT2 which are involved in the fatty acid metabolism pathway and are central to effective energy processing in cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-ACADVL/VLCAD antibody (ab155138)
7.5% SDS PAGE
All lanes: Western blot - Anti-ACADVL/VLCAD antibody (ab155138) at 1/1000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 70 kDa
Western blot - Anti-ACADVL/VLCAD antibody (ab155138)
7.5% SDS PAGE
All lanes: Western blot - Anti-ACADVL/VLCAD antibody (ab155138) at 1/3000 dilution
All lanes: Mouse liver whole cell lysate at 50 µg
Predicted band size: 70 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ACADVL/VLCAD antibody (ab155138)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling ACADVL/VLCAD with ab155138 at 1/200. Image at the bottom costained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody (ab155138)
Immunohistochemical analysis of paraffin embedded H358 xenograft labeling ACADVL/VLCAD with ab155138 antibody at 1/250.
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