AB155138

Anti-ACADVL/VLCAD antibody

Name: Anti-ACADVL/VLCAD antibody (ab155138) | Abcam
Brand: Abcam Limited
SKU: AB155138
Price: 525 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(13 Publications)

Rabbit Polyclonal ACADVL/VLCAD antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACADVL aa 1-350.

View Alternative Names

VLCAD, ACADVL

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-ACADVL/VLCAD antibody (AB155138)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ACADVL/VLCAD antibody (AB155138)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling ACADVL protein at mitochondria using ab155138 at a 1/500 dilution (Green). DAPI was used for nuclear staining (Blue).
Scale bar = 10 µM

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody (AB155138)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody (AB155138)

Immunohistochemical analysis of paraffin-embedded human a549 xenograft tissue stained for ACADVL protein at mitochondria with ab155138 at a 1/500 dilution.

Antigen retrieval : EDTA based, pH 8.0 buffer, 15min

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody (AB155138)

Immunohistochemical analysis of paraffin embedded H358 xenograft labeling ACADVL/VLCAD with ab155138 antibody at 1/250.

Supplier Data

Western blot - Anti-ACADVL/VLCAD antibody (AB155138)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-ACADVL/VLCAD antibody (ab155138) at 1/1000 dilution

Lane 1:

Wild-type ACADVL 293T cell extracts at 30 µg

Lane 2:

Knockout ACADVL 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 70 kDa

false

Supplier Data

Western blot - Anti-ACADVL/VLCAD antibody (AB155138)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-ACADVL/VLCAD antibody (ab155138) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 70 kDa

false

Supplier Data

Western blot - Anti-ACADVL/VLCAD antibody (AB155138)

Samples were separated by 7.5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-ACADVL/VLCAD antibody (ab155138) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 70 kDa

false

Unknown

Western blot - Anti-ACADVL/VLCAD antibody (AB155138)

7.5% SDS PAGE

All lanes:

Western blot - Anti-ACADVL/VLCAD antibody (ab155138) at 1/3000 dilution

All lanes:

Mouse liver whole cell lysate at 50 µg

Predicted band size: 70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ACADVL aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

P49748

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACADVL also known as VLCAD (Very Long-Chain Acyl-CoA Dehydrogenase) is an enzyme that plays an important role in the breakdown of long-chain fatty acids within the mitochondria. It has a molecular mass of approximately 70 kDa. VLCAD is expressed in various tissues with high levels found in the heart skeletal muscle and liver. These tissues are heavily reliant on fatty acid oxidation for energy production especially when glucose availability is low.

Biological function summary

ACADVL functions within the mitochondrial matrix where it catalyzes the initial step of beta-oxidation by desaturating long-chain acyl-CoA molecules. This enzyme is a part of a larger complex that includes other mitochondrial dehydrogenases contributing to the degradation of fatty acids into acetyl-CoA units. The process is essential for energy production especially under fasting conditions or high-energy demand scenarios such as muscle contraction and cardiac function.

Pathways

VLCAD plays an essential role in the fatty acid beta-oxidation pathway. It works alongside other enzymes such as ACADM (Medium-Chain Acyl-CoA Dehydrogenase) to facilitate the sequential shortening of fatty acids which integrates into the Krebs cycle for further energy extraction. This pathway maximizes energy yield from fatty acids significantly influencing cellular metabolism and energy homeostasis.

VLCAD deficiencies lead to severe metabolic conditions such as VLCAD deficiency. This disorder results in the impaired breakdown of long-chain fatty acids causing energy deficiency and the accumulation of toxic substances. Symptoms range from muscle weakness to serious cardiac complications. VLCAD deficiency's impact is often assessed in relation to proteins like ACSL1 and CPT2 which are involved in the fatty acid metabolism pathway and are central to effective energy processing in cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (PubMed : 18227065, PubMed : 7668252, PubMed : 9461620, PubMed : 9599005, PubMed : 9839948). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (PubMed : 18227065, PubMed : 7668252, PubMed : 9461620, PubMed : 9839948). Among the different mitochondrial acyl-CoA dehydrogenases, very long-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 12 to 24 carbons long primary chains (PubMed : 21237683, PubMed : 9839948).

See full target information ACADVL

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:14576 PubMed36028747

2022

PERM1 regulates genes involved in fatty acid metabolism in the heart by interacting with PPARα and PGC-1α.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yang Huang,Shin-Ichi Oka,Xiaoyong Xu,Chian-Feng Chen,Chien-Yi Tung,Ya-Yuan Chang,Youssef Mourad,Omair Vehra,Andreas Ivessa,Ghassan Yehia,Peter Romanienko,Chiao-Po Hsu,Junichi Sadoshima

Cellular and molecular life sciences : CMLS 79:375 PubMed35727412

2022

Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Zhi Peng,Yong-Xian Shao,Xiu-Zhen Li,Kang-Di Zhang,Yan-Na Cai,Yun-Ting Lin,Min-Yan Jiang,Zong-Cai Liu,Xue-Ying Su,Wen Zhang,Xiao-Ling Jiang,Li Liu

Autophagy 18:1367-1384 PubMed34689722

2021

Muscle-generated BDNF (brain derived neurotrophic factor) maintains mitochondrial quality control in female mice.

Applications

Unspecified application

Species

Unspecified reactive species

Palak Ahuja,Chun Fai Ng,Brian Pak Shing Pang,Wing Suen Chan,Margaret Chui Ling Tse,Xinyi Bi,Hiu-Lam Rachel Kwan,Daniel Brobst,Oana Herlea-Pana,Xiuying Yang,Guanhua Du,Suchaorn Saengnipanthkul,Hye Lim Noh,Baowei Jiao,Jason K Kim,Chi Wai Lee,Keqiang Ye,Chi Bun Chan

Journal of Zhejiang University. Science. B 21:885-896 PubMed33150772

2020

Novel ACADVL variants resulting in mitochondrial defects in long-chain acyl-CoA dehydrogenase deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Chen,Fan Tong,Xiao-Yu Wu,Ling Zhu,Qiu-Zi Yi,Jing Zheng,Ru-Lai Yang,Zheng-Yan Zhao,Xiao-Hui Cang,Qiang Shu,Ping-Ping Jiang

Theranostics 10:8197-8210 PubMed32724466

2020

Brown adipose tissue-derived exosomes mitigate the metabolic syndrome in high fat diet mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xueying Zhou,Zhelong Li,Meihao Qi,Ping Zhao,Yunyou Duan,Guodong Yang,Lijun Yuan

Molecular metabolism 37:100981 PubMed32283081

2020

Mitochondrial disease disrupts hepatic allostasis and lowers the threshold for immune-mediated liver toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Maxim Jestin,Senta M Kapnick,Tatyana N Tarasenko,Cassidy T Burke,Patricia M Zerfas,Francisca Diaz,Hilary Vernon,Larry N Singh,Ronald J Sokol,Peter J McGuire

American journal of physiology. Endocrinology and metabolism 318:E145-E151 PubMed31794263

2019

Combined overexpression of SIRT1 and knockout of GCN5 in adult skeletal muscle does not affect glucose homeostasis or exercise performance in mice.

Applications

Species

Mouse

Kristoffer Svensson,Shahriar Tahvilian,Vitor F Martins,Jessica R Dent,Adrianna Lemanek,Neeka Barooni,Keenan Greyslak,Carrie E McCurdy,Simon Schenk

Oxidative medicine and cellular longevity 2019:9894238 PubMed31737181

2019

Kynurenine, a Tryptophan Metabolite That Increases with Age, Induces Muscle Atrophy and Lipid Peroxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Helen Kaiser,Kanglun Yu,Chirayu Pandya,Bharati Mendhe,Carlos M Isales,Meghan E McGee-Lawrence,Maribeth Johnson,Sadanand Fulzele,Mark W Hamrick

Bioscience reports 39: PubMed30867255

2019

Distinct metabolic patterns during microglial remodeling by oleate and palmitate.

Applications

Unspecified application

Species

Unspecified reactive species

Bruno Chausse,Pamela A Kakimoto,Camille C Caldeira-da-Silva,Adriano B Chaves-Filho,Marcos Y Yoshinaga,Railmara Pereira da Silva,Sayuri Miyamoto,Alicia J Kowaltowski

PloS one 14:e0211733 PubMed30716103

2019

Resilient hepatic mitochondrial function and lack of iNOS dependence in diet-induced insulin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Pamela A Kakimoto,Bruno Chausse,Camille C Caldeira da Silva,José Donato Júnior,Alicia J Kowaltowski

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ACADVL/VLCAD antibody

Immunocytochemistry/ Immunofluorescence - Anti-ACADVL/VLCAD antibody (AB155138)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody (AB155138)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody (AB155138)

Western blot - Anti-ACADVL/VLCAD antibody (AB155138)

Western blot - Anti-ACADVL/VLCAD antibody (AB155138)

Western blot - Anti-ACADVL/VLCAD antibody (AB155138)

Western blot - Anti-ACADVL/VLCAD antibody (AB155138)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

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