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Rabbit Recombinant Monoclonal ACADVL/VLCAD antibody. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.


Images

Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (AB188872), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (AB188872), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (AB188872), expandable thumbnail
  • Immunoprecipitation - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (AB188872), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (AB188872), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/40

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/160

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Human

Dilution info

1/250 - 1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (PubMed:18227065, PubMed:7668252, PubMed:9461620, PubMed:9599005, PubMed:9839948). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (PubMed:18227065, PubMed:7668252, PubMed:9461620, PubMed:9839948). Among the different mitochondrial acyl-CoA dehydrogenases, very long-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 12 to 24 carbons long primary chains (PubMed:21237683, PubMed:9839948).

Alternative names

VLCAD, ACADVL, VLCAD, VLCAD

Recommended products

Rabbit Recombinant Monoclonal ACADVL/VLCAD antibody. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR15107(B)

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ACADVL also known as VLCAD (Very Long-Chain Acyl-CoA Dehydrogenase) is an enzyme that plays an important role in the breakdown of long-chain fatty acids within the mitochondria. It has a molecular mass of approximately 70 kDa. VLCAD is expressed in various tissues with high levels found in the heart skeletal muscle and liver. These tissues are heavily reliant on fatty acid oxidation for energy production especially when glucose availability is low.

Biological function summary

ACADVL functions within the mitochondrial matrix where it catalyzes the initial step of beta-oxidation by desaturating long-chain acyl-CoA molecules. This enzyme is a part of a larger complex that includes other mitochondrial dehydrogenases contributing to the degradation of fatty acids into acetyl-CoA units. The process is essential for energy production especially under fasting conditions or high-energy demand scenarios such as muscle contraction and cardiac function.

Pathways

VLCAD plays an essential role in the fatty acid beta-oxidation pathway. It works alongside other enzymes such as ACADM (Medium-Chain Acyl-CoA Dehydrogenase) to facilitate the sequential shortening of fatty acids which integrates into the Krebs cycle for further energy extraction. This pathway maximizes energy yield from fatty acids significantly influencing cellular metabolism and energy homeostasis.

Associated diseases and disorders

VLCAD deficiencies lead to severe metabolic conditions such as VLCAD deficiency. This disorder results in the impaired breakdown of long-chain fatty acids causing energy deficiency and the accumulation of toxic substances. Symptoms range from muscle weakness to serious cardiac complications. VLCAD deficiency's impact is often assessed in relation to proteins like ACSL1 and CPT2 which are involved in the fatty acid metabolism pathway and are central to effective energy processing in cells.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872), expandable thumbnail

    Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

    Lanes 1-4: Merged signal (red and green). Green - ab188872 observed at 70 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab188872 Anti-ACADVL/VLCAD antibody [EPR15107(B)] was shown to specifically react with ACADVL/VLCAD in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human ACADVL (VLCAD) knockout HEK-293T cell line ab266484 (knockout cell lysate Human ACADVL (VLCAD) knockout HEK-293T cell lysate ab257332) was used. Wild-type and ACADVL/VLCAD knockout samples were subjected to SDS-PAGE. ab188872 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: ACADVL knockout HEK293T cell lysate at 20 µg

    Lane 3: A431 cell lysate at 20 µg

    Lane 4: HepG2 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 70 kDa

    Observed band size: 70 kDa

    This data was developed using the same antibody clone in a different buffer formulation (ab188872).

    Lanes 1-4: Merged signal (red and green). Green - ab188872 observed at 70 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab188872 Anti-ACADVL/VLCAD antibody [EPR15107(B)] was shown to specifically react with ACADVL/VLCAD in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human ACADVL (VLCAD) knockout HEK-293T cell line ab266484 (knockout cell lysate Human ACADVL (VLCAD) knockout HEK-293T cell lysate ab257332) was used. Wild-type and ACADVL/VLCAD knockout samples were subjected to SDS-PAGE. ab188872 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry (Intracellular) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

    Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling ACADVL/VLCAD with ab188872 at 1/160 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

    Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling ACADVL/VLCAD with ab188872 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872), expandable thumbnail

    Immunoprecipitation - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

    Western blot analysis of immunoprecipitation pellet from A431 lysate immunoprecipitated using ab188872 at 1/50 dilution.

    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

    All lanes: Immunoprecipitation - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

    Predicted band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ACADVL/VLCAD with ab188872 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872), expandable thumbnail

    Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

    All lanes: Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872) at 1/1000 dilution

    All lanes: 293 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 70 kDa

  • Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872), expandable thumbnail

    Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

    All lanes: Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872) at 1/10000 dilution

    Lane 1: HeLa cell lysate at 20 µg

    Lane 2: HepG2 cell lysate at 20 µg

    Lane 3: A431 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 70 kDa

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Product protocols

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