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AB188872

Anti-ACADVL/VLCAD antibody [EPR15107(B)]

  • 20ul selling size
  • RabMAb
  • Recombinant
  • KO Validated
  • What is this?

5

(1 Review)

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(3 Publications)

Rabbit Recombinant Monoclonal ACADVL/VLCAD antibody. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 3 publications.

View Alternative Names

VLCAD, ACADVL

7 Images
Flow Cytometry (Intracellular) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (AB188872)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (AB188872)

Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling ACADVL/VLCAD with ab188872 at 1/160 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (AB188872)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (AB188872)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ACADVL/VLCAD with ab188872 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (AB188872)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (AB188872)

Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling ACADVL/VLCAD with ab188872 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (AB188872)
  • IP

Supplier Data

Immunoprecipitation - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (AB188872)

Western blot analysis of immunoprecipitation pellet from A431 lysate immunoprecipitated using ab188872 at 1/50 dilution.

Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

Predicted band size: 70 kDa

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Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (AB188872)
  • WB

Supplier Data

Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (AB188872)

All lanes:

Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 70 kDa

false

Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (AB188872)
  • WB

Supplier Data

Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (AB188872)

All lanes:

Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872) at 1/1000 dilution

All lanes:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 70 kDa

false

Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (AB188872)
  • WB

Lab

Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (AB188872)

Lanes 1-4 : Merged signal (red and green). Green - ab188872 observed at 70 kDa. Red - loading control ab8245 observed at 36 kDa.

ab188872 Anti-ACADVL/VLCAD antibody [EPR15107(B)] was shown to specifically react with ACADVL/VLCAD in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266484 (knockout cell lysate ab257332) was used. Wild-type and ACADVL/VLCAD knockout samples were subjected to SDS-PAGE. ab188872 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

ACADVL knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human ACADVL (VLCAD) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-acadvl-vlcad-knockout-hek-293t-cell-line-ab266484'>ab266484</a>)

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 70 kDa

Observed band size: 70 kDa

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  • Carrier free

    Anti-ACADVL/VLCAD antibody [EPR15107(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15107(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody (AB155138)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/160", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACADVL also known as VLCAD (Very Long-Chain Acyl-CoA Dehydrogenase) is an enzyme that plays an important role in the breakdown of long-chain fatty acids within the mitochondria. It has a molecular mass of approximately 70 kDa. VLCAD is expressed in various tissues with high levels found in the heart skeletal muscle and liver. These tissues are heavily reliant on fatty acid oxidation for energy production especially when glucose availability is low.
Biological function summary

ACADVL functions within the mitochondrial matrix where it catalyzes the initial step of beta-oxidation by desaturating long-chain acyl-CoA molecules. This enzyme is a part of a larger complex that includes other mitochondrial dehydrogenases contributing to the degradation of fatty acids into acetyl-CoA units. The process is essential for energy production especially under fasting conditions or high-energy demand scenarios such as muscle contraction and cardiac function.

Pathways

VLCAD plays an essential role in the fatty acid beta-oxidation pathway. It works alongside other enzymes such as ACADM (Medium-Chain Acyl-CoA Dehydrogenase) to facilitate the sequential shortening of fatty acids which integrates into the Krebs cycle for further energy extraction. This pathway maximizes energy yield from fatty acids significantly influencing cellular metabolism and energy homeostasis.

VLCAD deficiencies lead to severe metabolic conditions such as VLCAD deficiency. This disorder results in the impaired breakdown of long-chain fatty acids causing energy deficiency and the accumulation of toxic substances. Symptoms range from muscle weakness to serious cardiac complications. VLCAD deficiency's impact is often assessed in relation to proteins like ACSL1 and CPT2 which are involved in the fatty acid metabolism pathway and are central to effective energy processing in cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation (FAO), breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (PubMed : 17564966, PubMed : 18227065, PubMed : 7668252, PubMed : 9461620, PubMed : 9599005, PubMed : 9839948). The first step of FAO consists in the proR-proR stereospecific alpha, beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA) (PubMed : 18227065, PubMed : 7668252, PubMed : 9461620, PubMed : 9839948). Among the different mitochondrial acyl-CoA dehydrogenases, very long-chain specific acyl-CoA dehydrogenase acts specifically on fatty acyl-CoAs with saturated 12 to 24 carbons long primary chains (PubMed : 17564966, PubMed : 21237683, PubMed : 9839948).
See full target information ACADVL
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell stem cell 29:559-576.e7 PubMed35325615

2022

PPARdelta activation induces metabolic and contractile maturation of human pluripotent stem cell-derived cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Nadeera M Wickramasinghe,David Sachs,Bhavana Shewale,David M Gonzalez,Priyanka Dhanan-Krishnan,Denis Torre,Elizabeth LaMarca,Serena Raimo,Rafael Dariolli,Madhavika N Serasinghe,Joshua Mayourian,Robert Sebra,Kristin Beaumont,Srinivas Iyengar,Deborah L French,Arne Hansen,Thomas Eschenhagen,Jerry E Chipuk,Eric A Sobie,Adam Jacobs,Schahram Akbarian,Harry Ischiropoulos,Avi Ma'ayan,Sander M Houten,Kevin Costa,Nicole C Dubois

Frontiers in physiology 12:773995 PubMed34975527

2021

Frequent Manipulation of Resistance Training Variables Promotes Myofibrillar Spacing Changes in Resistance-Trained Individuals.

Applications

Unspecified application

Species

Unspecified reactive species

Carlton D Fox,Paulo H C Mesquita,Joshua S Godwin,Vitor Angleri,Felipe Damas,Bradley A Ruple,Casey L Sexton,Michael D Brown,Andreas N Kavazis,Kaelin C Young,Carlos Ugrinowitsch,Cleiton A Libardi,Michael D Roberts

The Journal of clinical investigation 131: PubMed33945503

2021

C2orf69 mutations disrupt mitochondrial function and cause a multisystem human disorder with recurring autoinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Lausberg,Sebastian Gießelmann,Joseph P Dewulf,Elsa Wiame,Anja Holz,Ramona Salvarinova,Clara D van Karnebeek,Patricia Klemm,Kim Ohl,Michael Mull,Till Braunschweig,Joachim Weis,Clemens J Sommer,Stephanie Demuth,Claudia Haase,Claudia Stollbrink-Peschgens,François-Guillaume Debray,Cecile Libioulle,Daniela Choukair,Prasad T Oommen,Arndt Borkhardt,Harald Surowy,Dagmar Wieczorek,Norbert Wagner,Robert Meyer,Thomas Eggermann,Matthias Begemann,Emile Van Schaftingen,Martin Häusler,Klaus Tenbrock,Lambert van den Heuvel,Miriam Elbracht,Ingo Kurth,Florian Kraft
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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